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首页> 外文学位 >Differential cell killing of normal and transformed human lung fibroblasts by reovirus.
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Differential cell killing of normal and transformed human lung fibroblasts by reovirus.

机译：呼肠孤病毒对正常和转化的人肺成纤维细胞的杀伤细胞分化作用。

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摘要

When infected with reovirus ST3, strain Dearing, normal and transformed human lung fibroblasts exhibit differential sensitivities. Transformed cells (WI38 VA13 2RA) are destroyed within four days. In contrast, normal cells (WI38) maintain a productive infection for as long as two months. Attempts to examine the differences between these cells included the use of cDNA subtraction and a reovirus sigma 1 protein affinity chromatography column, both of which were hampered by technical difficulties. Two-dimensional gel electrophoresis revealed patterns of differential protein expression between infected and mock-infected normal and transformed cells. Visual comparison of Coomassie blue-stained gels revealed one protein which was present in uninfected normal cells but was missing in all other samples, as well as five proteins that were present in infected and mock-infected normal cells but were missing in both transformed cell samples. Autoradiography of 35S-labeled cell samples revealed an additional eleven proteins not seen with Coomassie staining. Further characterization of these proteins should uncover the mechanism of differential cell killing by reovirus.
机译：当感染呼肠孤病毒ST3时，Dearing菌株，正常的和转化的人肺成纤维细胞表现出不同的敏感性。转化的细胞（WI38 VA13 2RA）在四天内被破坏。相比之下，正常细胞（WI38）可以维持生产性感染长达两个月。试图检查这些细胞之间的差异的尝试包括使用cDNA减法和呼肠孤病毒sigma 1蛋白亲和层析柱，这两种技术均受阻。二维凝胶电泳揭示了感染和模拟感染的正常细胞和转化细胞之间蛋白质表达差异的模式。视觉比较考马斯蓝染色的凝胶，发现一种蛋白质存在于未感染的正常细胞中，但在所有其他样品中均缺失，另外五种蛋白质存在于受感染和模拟感染的正常细胞中，但在两个转化细胞样品中均缺失。 35S标记的细胞样品的放射自显影显示了考马斯染色未见的另外11种蛋白质。这些蛋白质的进一步表征应揭示呼肠孤病毒杀死不同细胞的机制。

著录项

  • 作者

    Loeffler, Jennifer L.;

  • 作者单位

    Florida Atlantic University.;

  • 授予单位 Florida Atlantic University.;
  • 学科 Biology Molecular.;Biology Cell.
  • 学位 M.S.
  • 年度 2000
  • 页码 83 p.
  • 总页数 83
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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