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首页> 外文学位 >Development of thermally controllable protein columns and novel biological inhibitors for the mitigation of calcium carbonate fouling and scale formation on heat transfer surfaces through genetic engineering.
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Development of thermally controllable protein columns and novel biological inhibitors for the mitigation of calcium carbonate fouling and scale formation on heat transfer surfaces through genetic engineering.

机译：通过基因工程开发可热控制的蛋白质色谱柱和新型生物抑制剂，以减轻碳酸钙结垢和热传递表面结垢的形成。

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摘要

Calcium carbonate is one of the most common scales found in valves, pipes, and heat exchanger surfaces. Calcium-binding proteins have received much attention by scientists due to the important biological function of calcium ions as, among others, an intracellular messenger in many eukaryotic signal-transducing pathways, such as vision, the phosphoinositide cascade and the regulation of muscle contraction.;This work proposes an innovative method of fouling mitigation by protein and genetic engineering. It makes use of the strong affinity of proteins in binding and removing the key dissolved metal ion (in the fluid) that triggers scale formation on heat exchanger surfaces. It shows that by raising temperature to its melting temperature, a metal-binding protein with chelated metal ions can change its native (folded) conformational state to its denatured random-coiled polypeptide (unfolded) state, and that by raising a temperature higher than the melting temperature, the protein will release the chelated ions. It is further proven that by repeating the heating and cooling cycle, the protein can chelate and release the scale inducing metal ions, thus establishing the feasibility of developing a new class of effective biodegradable anti-foulants that are thermally regenerative and friendly to the environment.;Furthermore, with the new expression system constructed and the simplified purification procedure developed, an industrial process by fermentation for the mass production of calmodulin was successfully developed through genetic engineering. By the proposed process, a massive amount of genetically engineered calmodulin was produced for a series of essential experimental investigations, including (1) behavior of calmodulin column for pretreatment of a working fluid containing calcium ions, (2) immobilization of calmodulin on an inorganic support (SiO2) for high temperature application, and (3) inhibition of calmodulin for calcium carbonate scale formation on heat transfer surfaces.;The experimental results show (1) that calmodulin column does have the ability to remove calcium ions from a working fluid containing low level of calcium ions and (2) that calmodulin inhibitor does have the ability to mitigate calcium carbonate scale formation on heat transfer surfaces.
机译：碳酸钙是阀门，管道和热交换器表面上最常见的水垢之一。钙结合蛋白由于钙离子的重要生物学功能而受到科学家的广泛关注，钙离子结合蛋白是许多真核信号传导途径中的细胞内信使，例如视觉，磷酸肌醇级联和肌肉收缩的调节。这项工作提出了一种通过蛋白质和基因工程减轻结垢的创新方法。它利用蛋白质的强亲和力来结合和去除关键的溶解金属离子（在流体中），从而触发在换热器表面形成水垢。它表明，通过将温度提高到其解链温度，具有螯合金属离子的金属结合蛋白可以将其天然（折叠）构象状态更改为变性的随机螺旋多肽（未折叠）状态，并且通过将温度升高到高于在融化温度下，蛋白质会释放出螯合离子。进一步证明，通过重复加热和冷却循环，蛋白质可以螯合并释放引起水垢的金属离子，从而建立了开发新型的可热再生且对环境友好的有效的可生物降解的防污剂的可行性。 ；此外，通过构建新的表达系统和开发简化的纯化程序，通过基因工程成功开发了发酵生产钙调蛋白的工业方法。通过提出的方法，生产了大量的基因工程钙调蛋白，用于一系列基本的实验研究，包括（1）钙调蛋白柱对含钙离子的工作液进行预处理的行为，（2）钙调蛋白固定在无机载体上（SiO2）用于高温应用，以及（3）抑制钙调蛋白在传热表面上形成碳酸钙结垢的实验结果;实验结果表明（1）钙调蛋白柱确实能够从含有低钙的工作流体中去除钙离子钙离子水平和（2）钙调蛋白抑制剂确实具有减轻传热表面碳酸钙垢形成的能力。

著录项

  • 作者

    Chen, Tzer-Yi.;

  • 作者单位

    The University of Wisconsin - Milwaukee.;

  • 授予单位 The University of Wisconsin - Milwaukee.;
  • 学科 Engineering Mechanical.
  • 学位 Ph.D.
  • 年度 2000
  • 页码 181 p.
  • 总页数 181
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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