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首页> 外文学位 >Plant response to induced systemic resistance against blue mold disease in tobacco elicited by plant growth-promoting rhizobacteria.
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Plant response to induced systemic resistance against blue mold disease in tobacco elicited by plant growth-promoting rhizobacteria.

机译：植物对促进植物生长的根际细菌引起的烟草中对蓝霉病的系统抗性的响应。

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摘要

Five strains of plant growth-promoting rhizobacteria (PGPR) Serratia marcescens 90-166, Bacillus pumilus SE34, Pseudomonas fluorescens 89B-61, Bacillus pumilus T4, and Bacillus pasteurii C-9 which previously have induced systemic resistance (ISR) in some crops against several pathogens were evaluated for their potential to induce resistance against blue mold of tobacco caused by Peronospora tabacina. Lesion area was significantly reduced by these five tested PGPR strains in tobacco cultivars Xanthinc, Ky14 and TN90 in greenhouse assays. Sporulation of P. tabacina was also significantly reduced on PGPR-treated plants. The capacity of PGPR strains to increase plant growth was partly related to induced resistance capacity against blue mold disease.;A microtiter plate assay was developed in which PGPR were applied to the base of tobacco seedlings growing on MS agar, and the pathogen was applied as a drop of sporangiospore suspensions to top leaves. Blue mold disease severity was consistently reduced significantly by strains 90-166, SE34, and T4, while results with 89B-61 were variable. No disease reduction was obtained with C-9. PGPR-mediated ISR was associated with reduced numbers of infected leaves and reduced spore production of P. tabacina.;Trypan blue staining of P. tabacina-infected tobacco leaves from the microtiter plates demonstrated that the development of P. tabacina hyphae was restricted in leaf tissues, showing necrosis of plant cells and degradation of fungal hyphae in 90-166- and SE34-treated plants.;Endogenous free salicylic acid (SA) levels in PGPR-treated tobacco plants were detected using HPLC. During the first week after PGPR treatment, SA levels significantly increased in tobacco plants treated with strains 90-166, SE34 and 89B-61. However, SA levels in PGPR treated plants were significantly lower than in nontreated plants during the second week. With pathogen challenge at 7 days after PGPR treatment, the trend of SA levels was different in plants treated with different PGPR strains.;Application of these PGPR strains to NahG tobacco plants resulted in significant disease reduction. Northern analysis indicated that there was no difference in expression of PR-la and basic beta-1,3-glucanase genes between PGPR induced and noninduced plants. These results suggest that ISR mediated by tested PGPR strains is SA-independent and not associated with activation of pathogenesis-related (PR) protein genes.
机译：五株植物促生根瘤菌（PGPR）粘质沙雷氏菌90-166，短小芽孢杆菌SE34，荧光假单胞菌89B-61，短小芽孢杆菌T4和巴斯德芽孢杆菌C-9，它们先前已在某些作物中诱导了对某些作物的系统抗性（ISR）评估了几种病原体诱导烟草抗白粉病的潜在能力。在温室试验中，烟草品种Xanthinc，Ky14和TN90中这五个测试的PGPR菌株显着减少了病变面积。在PGPR处理的植物上，烟草的孢子形成也显着减少。 PGPR菌株增加植物生长的能力部分与诱导的抗蓝霉病能力有关。;建立了微量滴定板测定法，其中将PGPR应用于在MS琼脂上生长的烟草幼苗的基部，并将病原体用作一滴孢子囊悬浮液到叶顶。菌株90-166，SE34和T4显着降低了蓝霉病的严重程度，而89B-61的结果却不尽相同。 C-9不能减少疾病。 PGPR介导的ISR与减少的烟草叶片感染数量和孢子产生的减少相关;;台盼蓝染色从微量滴定板上感染烟草的烟草叶的台盼蓝染色表明，烟草中的烟草菌丝的发育受到限制在90-166和SE34处理的植物中显示出植物细胞的坏死和真菌菌丝的降解。;使用HPLC检测了PGPR处理的烟草植物中的内源性游离水杨酸（SA）水平。在PGPR处理后的第一周内，用菌株90-166，SE34和89B-61处理的烟草植物中的SA水平显着增加。但是，在第二周，PGPR处理过的植物中的SA水平明显低于未处理过的植物。 PGPR处理后第7天受到病原体攻击，用不同PGPR菌株处理过的植物中SA含量的变化趋势有所不同；将这些PGPR菌株应用于NahG烟草植物可显着减少病害。 Northern分析表明，PGPR诱导的植物和未诱导的植物之间PR-1a和碱性β-1，3-葡聚糖酶基因的表达没有差异。这些结果表明，由测试的PGPR菌株介导的ISR与SA无关，并且与发病相关（PR）蛋白基因的激活无关。

著录项

  • 作者

    Zhang, Shouan.;

  • 作者单位

    Auburn University.;

  • 授予单位 Auburn University.;
  • 学科 Agriculture Plant Pathology.
  • 学位 Ph.D.
  • 年度 2000
  • 页码 133 p.
  • 总页数 133
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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