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首页> 外文学位 >Construction of citrus genome maps with AFLP markers (Molecular markers).
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Construction of citrus genome maps with AFLP markers (Molecular markers).

机译：用AFLP标记（分子标记）构建柑橘基因组图。

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The Amplified Fragment Length Polymorphism (AFLP) marker system was used to construct a total citrus genome map based on an intergeneric backcross population of Citrus grandis (‘Thong Dee’) X USDA 17-40 (an F1 hybrid of C. grandis x Poncirus trifoliata). The map contains 337 DNA markers and a virus resistance gene (Ctv), and it was calculated by the JoinMap linkage analysis program. The map covers 858.6 cM of the citrus genome with 11 linkage groups, and an increased marker density of 2.5 cM/interval. A comparative analysis was conducted with the map based on AFLPs and one based on RAPD (random amplified polymorphic DNA) markers. Two maps were integrated, to generate a composite high-density citrus map with 509 markers of various types, covering a genetic distance of 1026.4 cM. The map density was compacted to 2.0 cM/interval. Map synteny analysis revealed genomic region rearrangements between major linkage groups of these two maps. Fifteen salt tolerance-related and 6 growth-related traits were selected for QTL analysis with MapQTL (JoinMap version) program. There were 36 putative QTLs found in association with growth-related traits, and 50 putative QTLs for salt tolerance-related traits. The possible genuine QTLs for those traits were analyzed further.; Marker-assisted selection was used to construct a localized genetic map for the Ctv gene region with AFLP markers. A subset of recombinants for Ctv-linked markers from a backcross population of Citrus and Poncirus were selected and subjected to CTV resistance phenotyping. Putative Ctv-linked AFLP markers were mapped, and the marker density in the Ctv region was increased. A consensus map was constructed based on three different mapping sources, which presented integrated map information of Ctv and a citrus nematode resistance gene region. These markers, along with those for salt tolerance, could be useful for marker-assisted selection in citrus rootstock breeding programs.
机译：使用扩增片段长度多态性（AFLP）标记系统，基于 Citrus grandis （'Thong Dee'）X USDA 17-40（F1杂种）的跨代回交种群，构建了整个柑橘基因组图 C。grandis x Poncirus trifoliata ）。该图包含337个DNA标记和一个病毒抗性基因（ Ctv ），由JoinMap连锁分析程序计算得出。该图谱涵盖了具有11个连接基团的柑橘基因组的858.6 cM，标记的密度增加了2.5 cM /间隔。使用基于AFLP的图和基于RAPD（随机扩增的多态DNA）标记的图进行了比较分析。整合了两个图谱，以产生具有各种类型的509个标记物的合成高密度柑橘图谱，涵盖了1026.4 cM的遗传距离。将图密度压实至2.0 cM /间隔。映射共性分析揭示了这两个映射的主要连锁群之间的基因组区域重排。使用MapQTL（JoinMap版本）程序选择了15个耐盐性和6个生长相关性状进行QTL分析。与生长相关性状相关的推定QTL有36个，与耐盐性相关性状的推定QTL有50个。这些特征可能的真实QTL进行了进一步分析。标记辅助选择用于构建带有AFLP标记的 Ctv 基因区域的局部遗传图谱。从柑桔和 Poncirus 的回交群体中选择 Ctv 连锁标记的重组子，并进行CTV抗性表型分析。绘制了假定的 Ctv 链接的AFLP标记，并增加了 Ctv 区域的标记密度。基于三个不同的图谱来源构建了一个共有图谱，提供了 Ctv 和一个柑橘线虫抗性基因区域的整合图谱信息。这些标记以及耐盐性标记可用于柑橘砧木育种计划中的标记辅助选择。

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