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首页> 外文学位 >A positional candidate gene approach to the discovery of quantitative trait loci on bovine chromosome 5.
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A positional candidate gene approach to the discovery of quantitative trait loci on bovine chromosome 5.

机译：一种定位候选基因方法，用于发现牛5号染色体上的数量性状基因座。

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摘要

Twenty-four microsatellite markers were screened across 701 members of the three generation Angus x Brahman "Angleton" pedigree. A genetic map based on meiotic exchange was constructed from the microsatellite data for Bos taurus chromosome 5 (BTA5). Phenotypic data for 64 growth and carcass merit traits were collected for 543 progeny produced and slaughtered for the project. Composite interval analyses of the genotypic and phenotypic data were performed to search for quantitative trait loci (QTL) on BTA5. QTL were detected (LOD > 3) in three regions of the chromosome. A QTL in the centromeric region appeared to affect quality grade and marbling. A QTL in the interstitial region of the chromosome affected birth weight, weaning weight, out-of-Angleton weight, ribeye area, kidney, pelvic and heart fat and dressing percent. A QTL in the telomeric region appeared to affect weaning weight and Warner Bratzler shear force.;PCR primers for all publicly available markers in the three critical intervals were used to screen a bovine bacterial artificial chromosome (BAC) library. Positive BAC clones were mapped to BTA5 by fluorescence in situ hybridization (FISH) and sequence analysis of the PCR products from each clone. Approximately 20 shotgun subclones of each validated BAC were sequenced and evaluated by BLAST similarity searches for homologs in the human sequence databases to define intervals in the human genome map to be screened for candidate genes that might be responsible for the QTL effects on BTA5.;The three BACs identified and subcloned in the centromeric region of BTA5 contained sequences of long and short interspersed repetitive sequences (LINEs and SINEs) but not homologs to human sequences. Results from the homology study in the interstitial region produced multiple ties to the human RH map and demonstrated that multiple chromosomal rearrangements separate human chromosomes 12 and 22 and BTA5. Sequences analyzed from the telomeric region of BTA5 were found to be homologous with a single region of human chromosome 22. This region contains the gene encoding platelet derived growth factor B (PDGFB) which influences myofibroblast growth in mice and is a candidate gene for the QTL affecting growth and tenderness in cattle.
机译：筛选了三代安格斯（Angus x Brahman）“安格尔顿”（Angleton）谱系的701个成员的二十四个微卫星标记。从金牛座5号染色体（BTA5）的微卫星数据构建了基于减数分裂交换的遗传图谱。为该项目生产和屠宰的543个后代收集了64种生长和car体品质特征的表型数据。进行基因型和表型数据的复合区间分析，以搜索BTA5上的数量性状基因座（QTL）。在染色体的三个区域中检测到QTL（LOD> 3）。着丝粒区域的QTL似乎影响质量等级和大理石花纹。染色体间隙区域的QTL影响出生体重，断奶体重，安格尔顿外体重，肋眼面积，肾脏，骨盆和心脏脂肪以及穿衣率。端粒区域的QTL似乎影响断奶体重和Warner Bratzler剪切力。在三个关键间隔内，使用所有公开标记的PCR引物筛选牛细菌人工染色体（BAC）文库。通过荧光原位杂交（FISH）和来自每个克隆的PCR产物的序列分析，将阳性BAC克隆定位到BTA5。每个人验证的BAC的大约20个shot弹枪亚克隆进行了测序，并通过BLAST相似性搜索对人类序列数据库中的同源物进行了评估，以定义人类基因组图中的间隔，以筛选可能对BTA5产生QTL影响的候选基因。在BTA5着丝粒区域中鉴定并亚克隆的三个BAC包含长短短的重复序列（LINE和SINE）序列，但不与人序列同源。在间隙区域进行同源性研究的结果与人类RH图谱建立了多重联系，并证明了多个染色体重排将人类12号和22号染色体与BTA5染色体分开。发现从BTA5的端粒区域分析的序列与人类22号染色体的单个区域同源。该区域包含编码血小板衍生生长因子B（PDGFB）的基因，该基因影响小鼠成肌纤维细胞的生长，并且是QTL的候选基因。影响牛的生长和嫩度。

著录项

  • 作者

    Brenneman, Rick Alan.;

  • 作者单位

    Texas A&M University.;

  • 授予单位 Texas A&M University.;
  • 学科 Biology Genetics.
  • 学位 Ph.D.
  • 年度 1999
  • 页码 71 p.
  • 总页数 71
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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