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首页> 外文学位 >Inheritance of resistance to common bunt (Tilletia caries and T. foetida) and identification of RAPD markers linked to bunt resistance in wheat.
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Inheritance of resistance to common bunt (Tilletia caries and T. foetida) and identification of RAPD markers linked to bunt resistance in wheat.

机译：对普通短打（Tilletia caries和T. foetida）的抗性遗传和与小麦抗短打相关的RAPD标记的鉴定。

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摘要

Common bunt causes yield loss and reduces grain quality in common and durum wheats in western Canada. In order to aid bunt resistance breeding, this study was conducted to investigate the race-specificity and the inheritance of resistance to common bunt and to identify RAPD markers linked to bunt resistance for use in marker-assisted selection. In the study on race specificity of resistance, significant differences in resistance to bunt among cultivars and in virulence among races were found. Race T1 was found to be the least virulent and T19 was the most virulent. Race-specific resistance was demonstrated in all experiments.; Both common (Kite and Triple Dirk) and spelt (RL5407 and SK0263) wheats were used for studies on the inheritance of resistance to bunt. Generation mean analysis showed that additive effects were the main genetic effects and dominance effects were not significant in any cross. Epistatic effects may exist in the cross Laura/Kite. Gene estimates from the qualitative analysis indicated that Triple Dirk and RLS407 may carry a single gene for resistance to race T1, T13 and L7. Kite may carry two genes and SK0263 carries either one or two genes for resistance to race T1. Heritability was estimated to be moderate to high (54%–90%) for bunt resistance to races T1, T13 and L7.; An allelic study demonstrated that RL5407 may not carry Bt2 Bt3, Bt6, Bt8 or Bt10 genes for bunt resistance. RL5407 and SK0263 likely carry the same gene for resistance to race T1, and this gene is different from the gene carried by SK0505. RL5407 possibly carries a gene conditioning resistance to both races T13 and L7 in addition to the one for resistance to race T1. The genes carried by Triple Dirk for resistance to races T1, T13 and L7 are different from each other. No cytoplasmic effect was found for resistance to race T1 in the crosses Genesis/RL5407 and RL5407/Genesis.; Two flanking RAPD markers linked to resistance to bunt race T1, UBCS48 590 (in repulsion) and UBC274988 (in coupling), were identified in Laura/RL5407. They had linkages of 9.1 ± 4.0 cM and 18.2 ± 5.6 cM, respectively.
机译：在加拿大西部，普通小穗会导致单产降低，并降低谷物品质。为了帮助进行抗性育种，本研究旨在研究种族特异性和对普通抗性的遗传，并鉴定与抗性相关的RAPD标记，以用于标记辅助选择。在抗性的种族特异性研究中，发现品种之间对短打的抗性和种族之间的毒力存在显着差异。发现T1种族的毒性最低，而T19的毒性最高。在所有实验中都证明了种族特异性抗性。普通小麦（风筝和三重德克）和拼写小麦（RL5407和SK0263）都用于研究对短打的抗性遗传。世代均值分析表明，加成效应是主要的遗传效应，优势效应在任何杂交中均不显着。交叉劳拉/风筝中可能存在上位性效应。从定性分析得出的基因估计值表明，Triple Dirk和RLS407可能携带单个基因来抵抗T1，T13和L7族。风筝可能携带两个基因，而SK0263携带一个或两个基因来抵抗种族T1。据估计，对T1，T13和L7种族的短打抗性的遗传力为中等至高（54％–90％）。一项等位基因研究表明，RL5407可能不携带 Bt2 Bt3，Bt6，Bt8 或 Bt10 基因。 RL5407和SK0263可能携带相同的抗T1种族基因，该基因不同于SK0505携带的基因。 RL5407除了对T1种族有抵抗力之外，还可能对T13和L7两个种族都具有基因调控抗性。 Triple Dirk携带的抗种族T1，T13和L7的基因彼此不同。在杂交Genesis / RL5407和RL5407 / Genesis中没有发现对种族T1的抗性的细胞质作用。在Laura / RL5407中鉴定出了两个与抗T1种族相关的RAPD标记UBCS48 590 （排斥）和UBC274 988 （耦合）。它们的连锁度分别为9.1±4.0 cM和18.2±5.6 cM。

著录项

  • 作者

    He, Chunlin.;

  • 作者单位

    The University of Saskatchewan (Canada).;

  • 授予单位 The University of Saskatchewan (Canada).;
  • 学科 Biology Genetics.; Agriculture Agronomy.
  • 学位 Ph.D.
  • 年度 1999
  • 页码 151 p.
  • 总页数 151
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;农学（农艺学）;
  • 关键词

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