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Phenotypic characterization of a Salmonella typhimurium dsbA null mutant and identification of factors that regulate the expression of the disulfide oxidoreductase DsbA (Salmonella typhimurium).

机译：鼠伤寒沙门氏菌dsbA空突变体的表型表征和调节二硫键氧化还原酶DsbA（鼠伤寒沙门氏菌）表达的因素的鉴定。

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Protein disulfide bond formation in Gram negative bacteria has been shown to be catalyzed by the various periplasmic and membrane proteins that make up a disulfide bond formation (dsb) system. We are studying the contribution of these periplasmic foldases to the folding of proteins in Salmonella typhimurium. The gene coding for the key enzyme in this system, disulfide oxidoreductase DsbA, had been cloned in our laboratory and it has been determined that DsbA is regulated in a growth-phase dependent manner in S. typhimurium . In this study three aspects of the dsb system were further explored. The role of DsbA in the phenomenon of resistance to cationic peptide exhibited by this organism was examined.; It was determined that the S. typhimurium dsbA null strain is more susceptible than the wild type strain to the effects of protamine sulfate, a cationic antimicrobial peptide that is considered to be a functional analogue of defensin. Although the exact mechanism of susceptibility is unknown, the disruption of disulfide bond formation was shown to affect the secreted, envelope and outer membrane protein profiles of the dsbA null strain. The approaches we undertook for the identification of the dsbB gene proved unsuccessful, however, we now know that a dsbB homologue does exist in S. typhimurium as it has been sequenced by the Washington University Genome Sequencing Centre. (Abstract shortened by UMI.)

机译：革兰氏阴性细菌中的蛋白质二硫键形成已显示出由构成二硫键形成（dsb）系统的各种周质和膜蛋白催化。我们正在研究这些周质折叠酶对鼠伤寒沙门氏菌中蛋白质折叠的贡献。已经在我们的实验室中克隆了编码该系统中关键酶二硫键氧化还原酶DsbA的基因，并确定DsbA在 S中以生长阶段依赖性方式被调控。鼠伤寒。在这项研究中，进一步探讨了dsb系统的三个方面。检验了DsbA在该生物体表现出的对阳离子肽的抗性现象中的作用。确定 S。鼠伤寒dsbA无效菌株比野生型菌株更易受硫酸鱼精蛋白的影响，硫酸鱼精蛋白是一种阳离子抗微生物肽，被认为是防御素的功能类似物。尽管敏感性的确切机理尚不清楚，但已表明破坏二硫键的形成会影响 dsbA 无效菌株的分泌，包膜和外膜蛋白谱。我们用于鉴定 dsbB 基因的方法证明是不成功的，但是，我们现在知道 dsbB 同源物确实存在于 S中。鼠伤寒，已由华盛顿大学基因组测序中心进行了测序。（摘要由UMI缩短。）

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作者
Ponnampalam, Thilaka Vadhanaa.;
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Queen's University at Kingston (Canada).;

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授予单位 Queen's University at Kingston (Canada).;
学科 Biology Microbiology.; Health Sciences Immunology.
学位 M.Sc.
年度 1999
页码 160 p.
总页数 160
原文格式 PDF
正文语种 eng
中图分类微生物学;预防医学、卫生学;
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专利

1. Characterization of SrgA, a Salmonella enterica Serovar Typhimurium Virulence Plasmid-Encoded Paralogue of the Disulfide Oxidoreductase DsbA, Essential for Biogenesis of Plasmid-Encoded Fimbriae [J] . C. W. Bouwman, M. Kohli, A. Killoran, Journal of bacteriology . 2003,第3期

机译：SrgA的表征，肠炎沙门氏菌鼠伤寒沙门氏菌毒力质粒编码的二硫键氧化还原酶DsbA的旁系同源物，对质粒编码的菌毛的生物发生至关重要。
2. Salmonella typhimurium DsbA is growth-phase regulated [J] . Goecke M., Gallant C., Suntharalingam P., FEMS Microbiology Letters . 2002,第2期

机译：鼠伤寒沙门氏菌DsbA受生长期调控
3. H-NS Represses Salmonella enterica Serovar Typhimurium dsbA Expression during Exponential Growth [J] . C. V. Gallant, T. Ponnampalam, H. Spencer, Journal of bacteriology . 2004,第4期

机译：H-NS抑制指数增长过程中肠炎沙门氏菌血清鼠伤寒dsbA表达。
4. Evaluation of Salmonella Typhimurium fecal shedding in pigs vaccinated with Salmonella Choleraesuis-Typhimurium vaccine via drinking water and challenged four weeks later [C] . Q. Steichen, R. A. Smiley, B. Fergen, Annual^Meeting of the American^Association^of^Swine^Veterinarians. . 2016

机译：评价猪沙门氏菌育鼠粪便患者在饮用水中用沙门氏菌胆管疫苗疫苗接种疫苗，并挑战四周后
5. Identification and characterization of the Salmonella enterica serovar Typhimurium disulfide oxidoreductase DsbA. [D] . Turcot, Isabelle. 1997

机译：鉴定和鉴定肠炎沙门氏菌血清二硫化鼠伤寒氧化还原酶DsbA。
6. Characterization of SrgA a Salmonella enterica Serovar Typhimurium Virulence Plasmid-Encoded Paralogue of the Disulfide Oxidoreductase DsbA Essential for Biogenesis of Plasmid-Encoded Fimbriae [O] . C. W. Bouwman, M. Kohli, A. Killoran, 2003

机译：SrgA沙门氏菌鼠伤寒鼠伤寒毒力的质粒编码的二硫键氧化还原酶DsbA的旁系同源物的表征质粒编码的菌毛的生物发生至关重要。
7. Characterization of SrgA, a Salmonella enterica Serovar Typhimurium Virulence Plasmid-Encoded Paralogue of the Disulfide Oxidoreductase DsbA, Essential for Biogenesis of Plasmid-Encoded Fimbriae [O] . Bouwman, C. W., Kohli, M., Killoran, A., 2003

机译：SrgA的表征，肠炎沙门氏菌鼠伤寒沙门氏菌毒力的质粒编码的二硫键氧化还原酶DsbA的旁系同源物，对质粒编码的菌毛的生物发生至关重要。

Phenotypic characterization of a Salmonella typhimurium dsbA null mutant and identification of factors that regulate the expression of the disulfide oxidoreductase DsbA (Salmonella typhimurium).

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