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首页> 外文学位 >Applications of optical spectroscopy in supramolecular systems: I. Probing the interactions between DNA and dendrimer; II. Characterization of metal complexes and their interactions with DNA.
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Applications of optical spectroscopy in supramolecular systems: I. Probing the interactions between DNA and dendrimer; II. Characterization of metal complexes and their interactions with DNA.

机译：光谱学在超分子系统中的应用：I.探测DNA与树状聚合物之间的相互作用；二。金属络合物的特征及其与DNA的相互作用。

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The applications of optical spectroscopic techniques were carried out in two types of supramolecular systems: DNA-dendrimer system and metal complex-DNA system.; Dendrimers are a new class of hyperbranched polymers, with unique structures and novel properties. The use of optical spectroscopic techniques to characterize dendrimer structures is reviewed in Chapter 1. Among their many potential applications, dendrimers can achieve high efficient gene transfection by transferring genes into cells. The current work is aimed to explore the fundamental mechanisms of dendrimer-mediated gene transfection. Chapter 2 focuses on the characterization of the DNA-dendrimer interactions by UV-Vis, CD, fluorescence spectroscopic techniques. The results indicate strong interactions between DNA and larger dendrimers. A binding model is proposed, in which DNA can wrap around higher generation dendrimers in a similar manner as DNA-histones. The results are also correlated to the gene transfection efficiencies of different dendrimer generations.; Ruthenium(II) dppz complexes and rhodium(III) phi complexes have been demonstrated to intercalate into DNA efficiently, and display interesting photochemical reactions with DNA, including fast photo-induced electron transfer through DNA, DNA photocleavage and photooxidation. In Chapter 3, work using resonance Raman spectroscopy helped to characterize the electronic structures of related metal complexes in solution and those bound to DNA. The results reveal important ground state and excited state properties of the metal complexes, as well as the strong pipi interaction between the intercalated ligand and DNA base stacking.
机译：光谱技术的应用在两种类型的超分子系统中进行：DNA-树状大分子系统和金属络合物-DNA系统。树枝状聚合物是一类新型的超支化聚合物，具有独特的结构和新颖的性能。第1章对使用光学光谱技术表征树状聚合物结构进行了综述。在其许多潜在应用中，树状聚合物可以通过将基因转移到细胞中来实现高效的基因转染。当前的工作旨在探索树枝状大分子介导的基因转染的基本机制。第2章重点介绍了通过UV-Vis，CD，荧光光谱技术表征DNA-树状聚合物的相互作用。结果表明DNA与较大的树枝状大分子之间有很强的相互作用。提出了一种结合模型，其中DNA可以以与DNA组蛋白相似的方式包裹更高年龄的树状聚合物。结果还与不同树状聚合物世代的基因转染效率相关。钌（II）dppz配合物和铑（III）phi配合物已被证明能有效地嵌入DNA中，并显示出与DNA有趣的光化学反应，包括通过DNA的快速光致电子转移，DNA光裂解和光氧化作用。在第3章中，使用共振拉曼光谱的工作有助于表征溶液中相关金属配合物以及与DNA结合的金属配合物的电子结构。结果揭示了金属配合物的重要基态和激发态特性，以及插层配体与DNA碱基堆积之间的强pipi相互作用。

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