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首页> 外文学位 >P450 aromatase expression and estradiol secretion in bovine granulosa cells in vitro.
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P450 aromatase expression and estradiol secretion in bovine granulosa cells in vitro.

机译：牛颗粒细胞中P450芳香化酶的表达和雌二醇的分泌。

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摘要

Folliculogenesis is a highly complex process which involves hormone-induced proliferation and differentiation of both theca and granulosa cells, leading ultimately to the increased ability of follicles to produce estradiol and to respond to gonadotropins. The ability to synthesize estradiol is an essential characteristic of a healthy ovarian follicle. Estradiol is synthesized in granulosa cells from thecal androgens; a process catalized by the enzyme P450 aromatase (P450arom). In cattle, the study of the hormonal regulation of P450arom gene expression and estradiol secretion has been hampered because of the lack of a cell model in which estradiol secretion can be maintained. Recently, a cell culture system has been developed in which bovine granulosa cells maintain aromatase activity in vitro. Thus, the aim of the present study was to examine the mechanisms involved in the regulation of P450arom gene expression and estradiol secretion by FSH, insulin and IGF-I in bovine granulosa cells in vitro.;The objective of the first study was to study the time course and the effects of FSH on P450arom, P450scc mRNAs, and estradiol and progesterone secretion. Estradiol secretion increased with time of culture, and this was correlated with an increase in P450arom mRNA abundance. Progesterone secretion also increased with time of culture, but P450 scc mRNA did not. We conclude that in the present culture cell model, P450arom mRNA and estradiol secretion can be maintained with low FSH concentrations, whereas higher doses of FSH downregulated P450arom mRNA levels. Contrary to P450arom mRNA, P450scc mRNA and progesterone secretion increased with highest FSH concentrations.;The objective of the second study was to determine the relative importance of FSH, IGF-I and insulin to maintain P450arom mRNA and estradiol secretion in cultured bovine granulosa cells.;The objective of the third study was to understand the intracellular mechanisms utilized by insulin and FSH to induce P450arom mRNA and estradiol secretion in granulosa cells.;The results of these studies showed that P450arom mRNA abundance and estradiol secretion can be induced and maintained by physiological FSH and insulin concentrations, and they are differentially regulated in bovine granulosa cells in vitro. (Abstract shortened by UMI.).
机译：卵泡形成是一个高度复杂的过程，涉及激素诱导的卵泡膜细胞和颗粒细胞的增殖和分化，最终导致卵泡产生雌二醇和对促性腺激素的反应能力增强。合成雌二醇的能力是健康的卵巢卵泡的基本特征。雌二醇是由鞘雄激素在颗粒细胞中合成的。由P450芳香酶（P450arom）催化的过程。在牛中，由于缺乏可以维持雌二醇分泌的细胞模型，因此对P450arom基因表达和雌二醇分泌的激素调节的研究受到阻碍。最近，已经开发了一种细胞培养系统，其中牛颗粒细胞在体外保持芳香酶活性。因此，本研究的目的是研究牛颗粒细胞中FSH，胰岛素和IGF-I调控P450arom基因表达和雌二醇分泌的调控机制。时程以及FSH对P450arom，P450scc mRNA，雌二醇和孕酮分泌的影响。雌二醇的分泌随培养时间的增加而增加，这与P450arom mRNA丰度的增加有关。孕酮的分泌也随着培养时间的增加而增加，但P450 scc mRNA却没有。我们得出的结论是，在目前的培养细胞模型中，低FSH浓度可以维持P450arom mRNA和雌二醇的分泌，而较高剂量的FSH可以下调P450arom mRNA的水平。与P450arom mRNA相反，在最高FSH浓度下，P450scc mRNA和孕酮分泌增加。；第二项研究的目的是确定FSH，IGF-I和胰岛素对于维持培养的牛颗粒细胞中P450arom mRNA和雌二醇分泌的相对重要性。 ；第三项研究的目的是了解胰岛素和FSH诱导颗粒细胞中P450arom mRNA和雌二醇分泌的细胞内机制。这些研究结果表明，生理上可以诱导和维持P450arom mRNA的丰度和雌二醇分泌。 FSH和胰岛素浓度，在体外牛颗粒细胞中受到差异调节。 （摘要由UMI缩短。）。

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  • 作者

    Silva Ramos, Jose Manuel.;

  • 作者单位

    Universite de Montreal (Canada).;

  • 授予单位 Universite de Montreal (Canada).;
  • 学科 Veterinary science.
  • 学位 Ph.D.
  • 年度 1999
  • 页码 219 p.
  • 总页数 219
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 肿瘤学;
  • 关键词

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