• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Dissociation of protonated peptides: Structure elucidation, energetics and mechanisms by tandem mass spectrometry.
【24h】

Dissociation of protonated peptides: Structure elucidation, energetics and mechanisms by tandem mass spectrometry.

机译：质子化肽的解离：串联质谱的结构阐明，能量学和机理。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In an effort to understanding peptide dissociation behavior in mass spectrometry, a thorough comparison study of surface-induced dissociation (SID) and collision-induced dissociation (CID) in the structural analysis of protonated peptides in the range of 300–1800 Da was conducted using tandem quadrupole and Fourier transform mass spectrometers (FTMS). Initially, on-resonance CID and sustained off-resonance irradiation (SORI)-CID processes were characterized by a “thermometer” molecule Cr(CO)6 in an FTMS. The average internal energy deposition and internal energy distributions were obtained by fitting the experimental data to the known threshold photonion photonelectron coincidence (TPIPECO) data using a computer algorithm. A comparison study of SID, SORI-CID and on-resonance CID shows that SID provides much higher average internal energies and narrower energy distributions than the other two methods.; A study of singly protonated pentapeptides showed that selective cleavages at the last residue of the C-terminus were observed in peptides containing no basic residues. It was proposed that acidic hydrogen at the C-terminal carboxylic acid could induce this preferential cleavage. The origins of a* and b* ions were determined by resonant ejection experiments. It was mainly from the fragmentation of [MH-NH3] + with a small fraction from the corresponding a and b ions.; The investigation of a set of angiotensin analogs show that charge state has dramatic effect on peptide dissociation. Non-selective cleavages were observed in singly protonated peptides while selective cleavages were obtained in doubly protonated peptides. Dominant cleavage at Pro in RVYIHPF indicates that alkylated amide nitrogens in the Pro enhance the formation of b/y singly protonated complementary ions.; An investigation of peptides containing two Asp or Glu residues and zero, one or two Arg shows the existence of acid-base interactions in the gas phase. The data illustrate that selective cleavages C-terminal to Asp were observed in both SORI-CID/FTMS and SID/quadrupole spectra. The preferential cleavage C-terminal to Glu observed in SORI-CID but not in SID suggests that the formation of 8-membered ring in Glu is rather slow and can only be observed in the long observation time window.
机译：为了理解质谱中的肽解离行为，在300-1800 Da范围内质子化肽的结构分析中，对表面诱导解离（SID）和碰撞诱导解离（CID）进行了彻底的比较研究。串联四极杆和傅立叶变换质谱仪（FTMS）。最初，通过FTMS中的“温度计”分子Cr（CO） 6 来表征共振CID和持续共振（SORI）-CID过程。通过使用计算机算法将实验数据拟合到已知的阈值光子离子光电子重合（TPIPECO）数据，可以获得平均内部能量沉积和内部能量分布。对SID，SORI-CID和共振CID的比较研究表明，与其他两种方法相比，SID提供了更高的平均内部能量和更窄的能量分布。对单质子化的五肽的研究表明，在不含碱性残基的肽中观察到了C末端最后一个残基的选择性切割。有人提出，C末端羧酸的酸性氢可以诱导这种优先裂解。通过共振喷射实验确定了 a * 和 b * 离子的起源。它主要是由[MH-NH 3 ] + 的碎片化所致，其中少量碎片来自相应的a和b离子。对一组血管紧张素类似物的研究表明，电荷状态对肽的解离具有显着影响。在单质子化的肽中观察到非选择性切割，而在双质子化的肽中获得选择性切割。 RVYIHPF中Pro处的显着裂解表明Pro中的烷基化酰胺氮可增强 b / y 单质子化的互补离子的形成。对包含两个Asp或Glu残基以及零个，一个或两个Arg的肽的研究表明，在气相中存在酸碱相互作用。数据表明，在SORI-CID / FTMS和SID /四极杆光谱中均观察到对Asp的C末端选择性切割。在SORI-CID中观察到Glu的C末端优先切割，但在SID中未观察到这表明在Glu中8元环的形成相当缓慢，并且只能在较长的观察时间窗内观察到。

著录项

  • 作者

    Zhong, Wenqing.;

  • 作者单位

    Virginia Commonwealth University.;

  • 授予单位 Virginia Commonwealth University.;
  • 学科 Chemistry Analytical.; Chemistry Organic.; Biophysics General.
  • 学位 Ph.D.
  • 年度 1999
  • 页码 416 p.
  • 总页数 416
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;有机化学;生物物理学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号