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首页> 外文学位 >Dalbergia and Albizia: Plantlet production via tissue culture, karyological evaluation, and seed anatomy with scanning electron microscopy.
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Dalbergia and Albizia: Plantlet production via tissue culture, karyological evaluation, and seed anatomy with scanning electron microscopy.

机译：黄檀和相思树：通过组织培养，核学评估和扫描电子显微镜观察的种子解剖结构来生产小苗。

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摘要

A publication by the American National Academy of Sciences (1979) outlined some of the urgent research need for a great variety of economically important woody species whose remaining genetic resources need urgently to be collected and conserved. I established a viable regeneration system via tissue and cell suspension culture for Albizia lebbeck and Albizia falcataria, two important wood yielding leguminous tree species. I standardized the culture medium by adding different growth factors and growth stimulators to obtain callus from the leaflet explants of the tree species. I found that the optimum use of casein hydrolysate (w/v) and coconut milk (v/v) in addition to 6-BAP and IBA could induce morphogenesis and somatic embryogenesis in the cultured tissue. This reports the first observation of somatic embryogenesis of A. lebbeck using leaflets as the explants. On MS media modified with 6-BAP (6 mg/l), NAA (0.02 mg/l), PVP (0.5%) and coconut milk (5% v/v) the explants produced proembryoids, other hormonal combinations produced various callus forms. I recorded the different stages of embryogenesis and plant development under the dissecting scope and SEM. After hardening and transferring, the trees grew more than 25 feet tall within few months outside the greenhouse.;The application of seed data is increasingly included and discussed in revisions and circumscription of angiosperm families and orders. I cut sections of the seeds of 24 species of 4 leguminous genera to study the seed anatomy under the dissecting microscope and the SEM. It was possible to observe the cellular organization of the epidermis, endosperm and internal structural details of the seeds on study with SEM. Main distinguishing characters observed were uniseriate or multiseriate epidermis, epidermal projections, and number of rows and nature of columns of hypodermal layer, especially the nature of endosperm. This study provided enough characteristic features to isolate the species that will help in further application of seed data in different fields.;I analyzed the karyotype of the three leguminous trees Albizia lebbeck, A. falcalaria and D. sissoo. I studied the somaclonal variation in the cultured tissue of D. sissoo. The aged tissue in culture develops certain cytological instabilities that are dependent upon the frequency of subculture as well as the age of the tissue. Using optimum concentrations of coconut milk and casein hydrolysate I could reduce Chromosomal abnormalities that could also reinstate the regeneration potential in the aged cultured tissue.
机译：美国国家科学院（1979）的出版物概述了对多种具有重要经济意义的木本物种的紧迫研究需求，这些木本物种的剩余遗传资源迫切需要收集和保存。我通过组织和细胞悬浮培养为Albizia lebbeck和Albizia falcataria建立了可行的再生系统，Albizia lebbeck和Albizia falcataria是两种重要的产生豆科树木的木材。我通过添加不同的生长因子和生长刺激剂来标准化培养基，以从树种的小叶外植体中获得愈伤组织。我发现除了6-BAP和IBA以外，酪蛋白水解物（w / v）和椰奶（v / v）的最佳使用可以在培养的组织中诱导形态发生和体细胞胚发生。这是首次报道以小叶为外植体的Leebeck体细胞胚发生。在用6-BAP（6 mg / l），NAA（0.02 mg / l），PVP（0.5％）和椰奶（5％v / v）修饰的MS培养基上，外植体产生前胚突，其他激素组合产生各种愈伤组织形式。我在解剖范围和扫描电镜下记录了胚胎发生和植物发育的不同阶段。经过硬化和转移后，树木在温室外的几个月内长高了25英尺以上。种子数据的应用越来越多地被包括在被子植物科和目的修订和限制中并进行了讨论。切开4个豆科植物24种种子的切片，在解剖显微镜和扫描电镜下研究种子的解剖结构。用SEM研究可以观察到表皮的细胞组织，胚乳和种子的内部结构细节。观察到的主要区别特征是单表皮或多表皮，表皮突起，皮下层的行数和列的性质，尤其是胚乳的性质。这项研究提供了足够的特征特征来分离该物种，这将有助于进一步在不同领域中应用种子数据。；我分析了三株豆科植物Albizia lebbeck，A。falcalaria和D. sissoo的核型。我研究了D. sissoo的培养组织中的体细胞克隆变异。培养中老化的组织发展出某些细胞学不稳定性，这取决于继代培养的频率以及组织的年龄。使用最佳浓度的椰奶和酪蛋白水解物I可以减少染色体异常，这也可以恢复老化培养组织中的再生潜力。

著录项

  • 作者

    Ghosh, Nabarun.;

  • 作者单位

    University of North Texas.;

  • 授予单位 University of North Texas.;
  • 学科 Biology Botany.;Agriculture Plant Culture.
  • 学位 Ph.D.
  • 年度 1998
  • 页码 162 p.
  • 总页数 162
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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