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首页> 外文学位 >Toward map-based cloning of fruit shape QTLs in tomato:fs8.1, a major QTL, sets the pattern of carpel shape well before anthesis; genetic characterization and high-resolution mapping ofovate.
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Toward map-based cloning of fruit shape QTLs in tomato:fs8.1, a major QTL, sets the pattern of carpel shape well before anthesis; genetic characterization and high-resolution mapping ofovate.

机译：转向基于图谱的番茄果实形状QTL克隆：主要的QTL fs8.1设定了在花期之前心皮形状的模式。卵子的遗传表征和高分辨率作图。

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摘要

fs8.1 is a major fruit shape QTL differentiating fresh market and processing tomatoes. Fine mapping was undertaken to determine whether the effect is due to a single gene or several tightly linked. For the 1,212 gametes screened in F2 and F3 families, it was determined that fs8.1 behaves as a single locus and maps to a cluster of RAPD and RFLP markers thought to flank the centromere of chromosome 8.;A comparative developmental study of fs8.1 NILs revealed that fs8.1 alleles exert their effects on fruit shape early in carpel development at least six days before anthesis.;A major locus controlling pear-shaped fruit in tomato was mapped to chromosome 2 in crosses between L. esculentum c.v. yellow pear TA503, and the round fruited wild species L. pimpinellifolium LA1589 (PM), as well as a cross between TA503 and the round fruit near isogenic line IL2-5 (PN) carrying the distal portion of chromosome 2 from the L. pennellii genome. The locus discovered in their crosses is mostly likely the same as the locus called ovate in the classical literature (Lindstrom 1926, 1927). ovate exerts its effect by changing oblate (round) fruit to an oval shape, and it might be the same gene causing the constriction of pear-shaped fruit in tomato. However, this could also be due to tightly linked genes.;High resolution genetic and physical maps of the region of chromosome 2 containing the major fruit shape determining locus, ovate, have been constructed. A total of 3000 NIL F2/F3 lines derived from TA503 x L. pennellii were used to fine map ovate. ovate is adjacent to the marker TG645 and flanked by TG463 and TG169 (0.7 cM interval). The marker TG645 was used to screen a BIBAC (Binary Bacterial Artificial Chromosome) library from L. pennellii. Four BIBAC clones potentially containing ovate were isolated. The map-based cloning of ovate will open the door to a better understanding of the molecular basis of fruit development, and also facilitate the genetic engineering of fruit shape characteristics.
机译：fs8.1是区分新鲜市场和加工番茄的主要水果形状QTL。进行了精细作图以确定该效应是由于单个基因还是几个紧密连锁。对于F2和F3家族中筛选的1,212个配子，已确定fs8.1表现为单个基因座并映射到认为位于8号染色体着丝粒侧翼的RAPD和RFLP标记簇; fs8的比较发育研究。 1 NILs显示fs8.1等位基因在花粉发育前至少六天对心形发育的早期果实产生影响。;番茄中一个控制梨形果实的主要基因座在第2号染色体上被绘制在番茄L. esculentum cv的杂交中黄梨TA503和圆形结果野生种L. pimpinellifolium LA1589（PM），以及TA503和近等基因系IL2-5（PN）的圆形结果之间的杂交，带有来自Pennellii的2号染色体的远端基因组。在他们的十字架上发现的基因座很可能与古典文献中称为卵形的基因座相同（Lindstrom 1926，1927）。卵形通过将扁圆的（圆形）水果变成椭圆形而发挥其作用，并且它可能是导致番茄中梨形水果收缩的同一个基因。但是，这也可能是由于紧密连接的基因所致。；已构建了包含主要水果形状决定基因座卵形的2号染色体区域的高分辨率遗传和物理图谱。总共使用3000个来自TA503 x L. pennellii的NIL F2 / F3品系精制卵形图。卵形与标志物TG645相邻，两侧为TG463和TG169（间隔0.7 cM）。标记TG645用于筛选来自L. pennellii的BIBAC（二进制细菌人工染色体）文库。分离了四个可能含有卵形的BIBAC克隆。卵形图的基于图的克隆将为更好地了解水果发育的分子基础打开一扇门，并且还有助于水果形状特征的基因工程。

著录项

  • 作者

    Ku, Hsin-Mei.;

  • 作者单位

    Cornell University.;

  • 授予单位 Cornell University.;
  • 学科 Biology Molecular.;Biology Botany.;Biology Genetics.
  • 学位 Ph.D.
  • 年度 1998
  • 页码 76 p.
  • 总页数 76
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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