Force studies of multiple kinesin-1 and Eg5 molecular motors.

机译：多种kinesin-1和Eg5分子马达的力研究。

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

Kinesin-1 and Eg5 are molecular motors that are essential for eukaryotic cell growth and development. Kinesin-1 motors transport intracellular cargo along the microtubule cytoskeleton through and against the dense viscoelastic cytoplasm. Eg5 motors mediate proper mitotic, microtubule spindle assembly during cell division, and stabilize these spindle structures with plus-end forces balancing opposing minus-end directed forces along the filaments. Therefore, it is important to understand how multiple kinesin-1 and Eg5 motors withstand competing forces, their structural rigidity under such loads, and how quickly they are able to rebind if loosed from their microtubule tracks. We get exact motor binding stoichiometry previously inaccessible by using a unique type of analysis for our optical trapping experiments. With this information, we characterize both motors and compare them to one another. We find the release forces for both types of motors add linearly with the number of motors bound and that Eg5 has a weaker binding than that of kinesin-1. The stiffness for the motor domains is comparable, yet we find that the average time to rebind for Eg5 decreases at higher concentrations while it does not for kinesin-1. This may indicate that there exists some weak interaction between kinesin-1 and microtubules keeping them close while unbound.

机译：Kinesin-1和Eg5是对真核细胞生长和发育必不可少的分子马达。 Kinesin-1电机沿着微管细胞骨架通过并逆着密集的粘弹性细胞质运输细胞内货物。 Eg5马达在细胞分裂过程中介导适当的有丝分裂，微管纺锤体组装，并通过平衡沿细丝相反的负端定向力的正向力稳定这些纺锤体结构。因此，重要的是要了解多个kinesin-1和Eg5电动机如何承受竞争力，在这种载荷下它们的结构刚性，以及如果从微管轨道上松开，它们能够以多快的速度重新结合。通过对光阱实验使用独特的分析类型，我们可以获得以前无法获得的精确的电机结合化学计量。利用这些信息，我们可以对两个电动机进行特性分析，并将它们相互比较。我们发现两种类型的电机的释放力都随绑定的电机数量线性增加，并且Eg5的绑定力比驱动蛋白1的绑定力弱。运动域的刚度是可比的，但是我们发现在较高的浓度下，Eg5的平均重结合时间会减少，而对于驱动蛋白-1的平均时间则不会。这可能表明驱动蛋白1和微管之间存在一些弱相互作用，使它们在未结合时保持紧密。

著录项

作者
Graves, Evan Taclibon.;
展开▼
作者单位

University of Illinois at Urbana-Champaign.;

展开▼
授予单位 University of Illinois at Urbana-Champaign.;
学科 Biology Molecular.;Physics Optics.;Biophysics General.
学位 Ph.D.
年度 2009
页码 87 p.
总页数 87
原文格式 PDF
正文语种 eng
中图分类分子遗传学;生物物理学;光学;
关键词

相似文献

外文文献
中文文献
专利

1. The molecular basis of frictional loads in the in vitro motility assay with applications to the study of the loaded mechanochemistry of molecular motors. [J] . Greenberg MJ, Moore JR Cytoskeleton . 2010,第5期

机译：体外运动分析中摩擦载荷的分子基础，并用于研究分子电动机的载荷机械化学。
2. The Contribution of the C-Terminal Tails of Microtubules in Altering the Force Production Specifications of Multiple Kinesin-1 [J] . Feizabadi Mitra Shojania Cell biochemistry and biophysics . 2016,第3期

机译：微管C末端尾巴在改变多种Kinesin-1的生产能力中的作用
3. Force Dependence of Velocity and Run Length of Kinesin-1, Kinesin-2 and Kinesin-5 Family Molecular Motors [J] . Guo Si-Kao, Wang Wei-Chi, Wang Peng-Ye, Molecules . 2019,第2期

机译：动力依赖速度和运行长度的Kinesin-1，Kinesin-2和Kinesin-5家族分子电机
4. EFFECTS OF INTERMOLECULAR FORCES ON THE STABILITY OF CARBON NANOTUBES CAPPED BY GOLD NANOPARTICLES AT ACIDIC PH. A MOLECULAR DYNAMICS STUDY [C] . Lukasz Konczak, Tomasz Panczyk World Conference on Carbon . 2016

机译：分子间力对酸性pH下金纳米颗粒盖碳纳米管稳定性的影响。分子动力学研究
5. Forces that Propel Serial Tech Startup Founders Forward in the Sequential Genesis of Multiple Tech Ventures: A Grounded Theory Study [D] . Ocsody, Peter. 2019

机译：推动串行技术启动创始人的力量在多种科技企业的连续创世纪前进：一个接地的理论研究
6. The role of thermal activation in motion and force generation by molecular motors. [O] . R D Astumian 2000

机译：热激活在分子电动机的运动和力产生中的作用。
7. The role of thermal activation in motion and force generation by molecular motors. [O] . Astumian, R D 2000

机译：热激活在分子电动机的运动和力产生中的作用。

Force studies of multiple kinesin-1 and Eg5 molecular motors.

摘要

著录项

相似文献

相关主题

期刊订阅