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An in vitro flow system to study the endothelial cell response to positive and negative wall shear stress gradients.

机译：一种体外流动系统，用于研究内皮细胞对正壁和负壁剪切应力梯度的响应。

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摘要

The apparent tendency of intracranial aneurysms to form at apices of cerebral bifurcations and outer sides of curved vessels, has led to many speculations that high wall shear stress (WSS) damages the arterial wall, starting from the inner layer: the endothelial cells (ECs). However, little is known about EC response to high WSS and wall shear stress gradients (WSSG). In this study the influence of WSS and WSSG on endothelial cell biology was examined under the hypothesis that initiation of intracranial aneurysms involves a local functional alteration in endothelium mediated by fluid shear stress and shear stress gradients. For this purpose, a flow chamber with converging and diverging sections was designed to expose cultured bovine aortic endothelial cells to positive and negative WSSGs. The chamber was designed such that effects of gradients at constant magnitude could be studied and simultaneously compared with zero gradient regions. The flow experiments ran for 24 hours. Endothelial cell morphology was investigated in relation to local flow characteristics and shear stress forces. Individual cell morphology and cell shape distribution in the monolayers were assessed using a computerized image analysis system. When exposed to wall shear stress gradients, cultured endothelial cells exhibited a dramatic response to characteristics of the flow. It was found that cells respond differently to negative and positive gradients. While in positive WSSG regions ECs aligned away from the flow, they were very well aligned with the flow in negative WSSG regions of nearly same magnitude. ECs were also found aligned with the flow in low WSS regions but were almost aligned perpendicular to the flow in high WSS regions. This investigation is one of the first to qualitatively compare effects of positive and negative WSSGs on vascular endothelial cells.
机译：颅内动脉瘤在脑分叉和弯曲血管外侧形成的明显趋势导致许多推测，即高壁切应力（WSS）从内层开始损害动脉壁：内皮细胞（ECs） 。但是，关于EC对高WSS和壁剪应力梯度（WSSG）的响应知之甚少。在这项研究中，在以下假设下检查了WSS和WSSG对内皮细胞生物学的影响：颅内动脉瘤的起始涉及由流体剪切应力和剪切应力梯度介导的内皮的局部功能改变。为了这个目的，设计了具有会聚和发散截面的流动室，以使培养的牛主动脉内皮细胞暴露于阳性和阴性WSSG。该室的设计使得可以研究恒定幅度的梯度影响，并同时与零梯度区域进行比较。流量实验进行了24小时。研究了内皮细胞形态与局部流动特性和切应力的关系。使用计算机图像分析系统评估单层细胞的单个细胞形态和细胞形状分布。当暴露于壁切应力梯度时，培养的内皮细胞对血流特征表现出戏剧性的响应。发现细胞对负和正梯度的反应不同。当在正WSSG区域中，EC远离流对齐时，它们与几乎相同大小的负WSSG区域中的流非常对齐。还发现EC与低WSS区域中的流对齐，但几乎垂直于高WSS区域中的流对齐。该研究是第一个定性比较阳性和阴性WSSGs对血管内皮细胞作用的研究之一。

著录项

  • 作者

    Singh, Sukhjinder.;

  • 作者单位

    State University of New York at Buffalo.;

  • 授予单位 State University of New York at Buffalo.;
  • 学科 Engineering Biomedical.;Engineering Mechanical.
  • 学位 M.S.
  • 年度 2009
  • 页码 81 p.
  • 总页数 81
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;机械、仪表工业;
  • 关键词

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