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Immune and endocrine modulations associated with enhanced plant-based protein diets within freshwater fish species.

机译:免疫和内分泌调节与淡水鱼种中植物性蛋白质饮食的增强有关。

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摘要

Two 60-day feeding trials with juvenile rainbow trout (Oncorhynchus mykiss) were conducted to compare the effects of fishmeal (FMC), defatted soybean meal (SBM), bioprocessed soybean meal (BSBM), and commercial soy protein concentrate (CSPC) ingredients on intestinal histology, innate immunity, and microbiota profiles. Intestinal lysozyme content was found to be highest at day 60 in the BSBM treatment group (P<0.01). No significant differences across treatments were observed in histological profiles at day 0 (P=0.75), day 15 (P=0.08), and day 60 (P=0.22). 16S rRNA microbiota analyses from collected fecal material indicated differences in alpha diversity among dietary groups, as demonstrated by Chao-1 (P<0.01) and Shannon indices (P=0.02). The incorporation of processed soy-based proteins alters the microbial community composition within the rainbow trout gastrointestinal tract (GIT), and modulates lysozyme concentrations within the distal gastrointestinal tissues.;A 60-day feeding trial was conducted to evaluate physiological changes from acute stressors in rainbow trout (Oncorhynchus mykiss) fed soy-based diets. Experimental diets contained two BSBM levels or a CSPC while dietary controls included a FM reference and a SBM. On days 30 and 60, trout were subjected to 4 hours of acute stressors (low flow rate, decreased dissolved oxygen and handling stressors) or left unstressed as controls for respective diet treatments. Day 30 changes in macrophage RBA were significantly different among diet treatments in the stressed (P=0.004) groups and between the stressed and unstressed groups at Day 60 (P=0.004).. Plasma cortisol results indicate that there were no dietary treatment differences but day 30 stressed (11.481.51 ng mL-1) and unstressed groups (0.470.28 ng mL-1) had a significant difference in group means (P<0.001). Day 60 plasma cortisol levels following a similar trend, significance between stressed (7.221.48 ng mL-1) and unstressed (0.360.12 ng mL-1) groups (P<0.001). Continued assessment of novel plant-based ingredients to reduce stressors may benefit producers by allowing increased stocking densities and reducing losses due to fish transport, thus further contributing to the needs of a rapidly expanding domestic aquaculture market.;Small additions of immunostimulants and the incorporation of bioprocessed soybean meal appear to modulate the fish immune system, the distal intestine microbiota, and aspects of stress physiology. These findings corroborate current aquaculture immunology initiatives to create non-antibiotic approaches that enhance fish health and increase production stocks in the rapidly expanding aquaculture sector. (Abstract shortened by ProQuest.).
机译:进行了两个为期60天的幼年虹鳟鱼(Oncorhynchus mykiss)喂养试验,以比较鱼粉(FMC),脱脂豆粕(SBM),生物加工豆粕(BSBM)和商业浓缩大豆蛋白浓缩物(CSPC)的影响。肠道组织学,先天免疫和微生物群谱。在BSBM治疗组中,肠道溶菌酶含量在第60天最高(P <0.01)。在第0天(P = 0.75),第15天(P = 0.08)和第60天(P = 0.22),在组织学特征上未观察到各治疗之间的显着差异。收集到的粪便中的16S rRNA微生物群分析表明,饮食组之间的α多样性存在差异,如Chao-1(P <0.01)和Shannon指数(P = 0.02)所示。掺入加工过的大豆基蛋白会改变虹鳟胃肠道(GIT)内的微生物群落组成,并调节远端胃肠道组织内的溶菌酶浓度。;进行了60天的喂养试验,以评估急性应激源引起的生理变化。虹鳟鱼(Oncorhynchus mykiss)以大豆为主食。实验饮食包含两个BSBM水平或CSPC,而饮食控制则包含FM参考和SBM。在第30天和第60天,对鳟鱼进行4个小时的急性应激(低流量,减少的溶解氧和处理应激源)或不加压力作为相应饮食疗法的对照。应激(P = 0.004)组的饮食治疗之间以及应激和非应激组在60天的饮食治疗之间,巨噬细胞RBA第30天的变化显着不同。血浆皮质醇的结果表明,饮食治疗没有差异,但是第30天有压力(11.481.51 ng mL-1)和无压力组(0.470.28 ng mL-1)的均值有显着差异(P <0.001)。第60天血浆皮质醇水平遵循相似的趋势,在应激(7.221.48 ng mL-1)和未应激(0.360.12 ng mL-1)组之间具有显着性(P <0.001)。继续评估新颖的植物成分以减少压力源,可以通过增加放养密度和减少鱼类运输造成的损失来使生产者受益,从而进一步满足迅速扩大的国内水产养殖市场的需求。经过生物处理的豆粕似乎可以调节鱼的免疫系统,远端肠道菌群和应激生理方面。这些发现证实了当前的水产养殖免疫学计划,旨在创造非抗生素方法,以增强鱼类健康并增加迅速发展的水产养殖部门的生产量。 (摘要由ProQuest缩短。)。

著录项

  • 作者

    Bruce, Timothy J.;

  • 作者单位

    South Dakota State University.;

  • 授予单位 South Dakota State University.;
  • 学科 Natural resource management.;Physiology.;Immunology.
  • 学位 Ph.D.
  • 年度 2016
  • 页码 247 p.
  • 总页数 247
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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