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首页> 外文学位 >Genetic fingerprinting of rice cultivars and genomic localization of the XA-5 gene for resistance to bacterial leaf blight of rice.
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Genetic fingerprinting of rice cultivars and genomic localization of the XA-5 gene for resistance to bacterial leaf blight of rice.

机译：水稻品种的遗传指纹图谱和XA-5基因的基因组定位，可抵抗水稻的细菌性叶枯病。

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摘要

The objectives of this research were to evaluate techniques for the rapid genetic characterization of cultivated rice (Oryza sativa L.) germplasm and to determine the genomic localization of the xa-5 gene for resistance to rice bacterial leaf blight (BLB).;Genetic and physical mapping were used to characterize the chromosomal region surrounding the xa-5 gene as a prelude to its positional cloning. Polymorphic molecular markers were identified in a parental survey of a nearly isogenic line cross. Recombination events around the gene were selected for by an efficient PCR-based screening of over one thousand F;Two physical contigs for the region were assembled based on bacterial (BAC) and yeast (YAC) artificial chromosome large-insert-clones. The BAC contig was constructed based on marker content and ISSR fingerprints of the individual clones. The interval with the targeted gene was spanned by a maximum of two BAC clones or either of two single YAC clones and the locus-specific estimates of the ratio of genetic to physical distance were very favorable for positional cloning.;The cloning of xa-5 should help to clarify some of the interesting central events of infection of rice by the BLB pathogen, Xanthomonas oryzae pv. oryzae, and will have implications for the study of recessive resistance genes in other pathosystems.;Three fingerprinting techniques, inter-simple sequence repeats (ISSR), amplified fragment length polymorphisms (AFLP), and fluorescently-labeled multiplexed microsatellites, were used to characterize varieties representative of the diversity of cultivated rice. These PCR-based genome scanning methods were able to identify Japonica versus Indica subspecies and to dissect finer levels of diversity. The ISSR analysis provided insights into the organization and frequency of different simple sequence repeats in the rice genome, while mapping of specific ISSR bands suggested that this technique produces many heritable markers useful for genetic analysis.
机译：这项研究的目的是评估栽培稻（Oryza sativa L.）种质的快速遗传特征鉴定技术，并确定对水稻白叶枯病（BLB）抗性的xa-5基因的基因组定位。物理作图被用来表征xa-5基因周围的染色体区域，作为其位置克隆的前奏。多态性分子标记是在父母对近等基因系杂交的调查中确定的。通过围绕1000多个F的高效PCR筛选选择基因周围的重组事件；基于细菌（BAC）和酵母（YAC）人工染色体大插入克隆，为该区域组装了两个物理重叠群。基于单个克隆的标记物含量和ISSR指纹构建BAC重叠群。靶向基因的间隔最多可跨越两个BAC克隆或两个单个YAC克隆中的一个，并且遗传距离与物理距离之比的基因座特异性估计对于位置克隆非常有利。; xa-5的克隆应该有助于弄清BLB病原体Xanthomonas oryzae pv感染水稻的一些有趣的中心事件。 ；将对其他病理系统中的隐性抗性基因的研究产生影响。；三种指纹技术，简单序列间重复（ISSR），扩增片段长度多态性（AFLP）和荧光标记的多重微卫星被用于表征代表水稻栽培品种多样性的品种。这些基于PCR的基因组扫描方法能够鉴定Japonica与Indica亚种，并分析更精细的多样性。 ISSR分析提供了有关水稻基因组中不同简单序列重复序列的组织和频率的见识，而特定ISSR条带的作图表明该技术产生了许多可用于遗传分析的可遗传标记。

著录项

  • 作者

    Blair, Matthew W.;

  • 作者单位

    Cornell University.;

  • 授予单位 Cornell University.;
  • 学科 Agriculture Agronomy.;Biology Genetics.;Agriculture Plant Pathology.
  • 学位 Ph.D.
  • 年度 1998
  • 页码 185 p.
  • 总页数 185
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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