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首页> 外文学位 >Part I. Direct injection of selected medications in serum using restricted access media columns. Part II. Capillary electrophoretic measurements of selected drugs in pharmaceutical dosage forms. Part III. Enantiomeric separations of medicinals using a protein-bonded chiral stationary phase.
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Part I. Direct injection of selected medications in serum using restricted access media columns. Part II. Capillary electrophoretic measurements of selected drugs in pharmaceutical dosage forms. Part III. Enantiomeric separations of medicinals using a protein-bonded chiral stationary phase.

机译：第一部分：使用限制进入介质的色谱柱直接在血清中注射选定的药物。第二部分药物剂型中所选药物的毛细管电泳测量。第三部分使用蛋白质键合的手性固定相分离药物的对映异构体。

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摘要

Chromatographic science and its recent advances have made it possible to examine some of the basic constituents of life. Evolvement of various modes of chromatography has contributed immensely in the study of protein, peptides, enzymes and nucleic acids in their purest state. High Performance Liquid Chromatography (HPLC) is the most widely used chromatographic technique today. Capillary electrophoresis (CE), because of its superior efficiencies and selectivity is becoming more popular and indeed has the potential to rival HPLC as an analytic tool in the near future. This dissertation work involved analytical method development of drug substances either in serum or dosage forms using High Performance Liquid Chromatography or Capillary Electrophoresis. Chapters 1--3 and chapters 7--9 describe analytical methods developed using HPLC. Chapters 4--6 describe analytical methods developed using capillary electrophoresis.;In chapters 1--3, direct serum injection methods were developed using Restricted Access Media HPLC columns for the determination of chlorzoxazone and its 6-hydroxy metabolite, phenylbutazone and its metabolite and some of the nonsteroidal antiinflammatory drugs (NSAIDs).;In chapter 4, a free solution capillary electrophoresis method was developed on fused silica capillaries to separate and quantitate codeine, caffeine, butalbital and aspirin in a mixture. In chapter 5, a capillary electrophoresis method was developed to separate and quantitate ephedrine (ED), theophylline (TP) and phenobarbital (PB) in a tablet dosage form.;In chapter 6, a micellar electrokinetic chromatography (MEKC) method was developed to analyze acetaminophen (AP), caffeine (CF) and butalbital (BB) simultaneously in a commercial tablet dosage form.;In chapter 7, methods were developed to separate dacarbazine and its two related impurities, 5-amino-imidazole-4 carboxamide (AIC) and 2-azahypoxanthine (AHX) by capillary electrophoresis and high performance liquid chromatography.;In chapter 8, an HPLC method was developed to determine racemic thalidomide in plasma on a avidin protein column using solid phase extraction.;In chapter 9, a commercially available avidin protein column was investigated for chiral separation of selected pharmaceuticals. Baseline resolution of enantiomers were successfully achieved for thalidomide, glutethimide, primaquine, aminoglutethimide, hydroxyzine, chlorthalidone and pyridoglutethimide.
机译：色谱科学及其最新进展使人们有可能研究生命的一些基本成分。各种色谱模式的发展为蛋白质，多肽，酶和核酸最纯净状态的研究做出了巨大贡献。高效液相色谱（HPLC）是当今使用最广泛的色谱技术。毛细管电泳（CE）由于其优越的效率和选择性正变得越来越流行，并且确实有可能在不久的将来与HPLC竞争，成为分析工具。本论文的工作涉及使用高效液相色谱或毛细管电泳分析血清或剂型中药物的分析方法。第1--3章和第7--9章介绍了使用HPLC开发的分析方法。第4--6章介绍了使用毛细管电泳开发的分析方法;第1--3章使用限制访问介质HPLC色谱柱开发了直接血清注射方法，用于测定氯唑沙宗及其6-羟基代谢产物，苯基丁but及其代谢产物和一些非甾体类抗炎药（NSAIDs）。在第4章中，开发了一种在熔融石英毛细管上的自由溶液毛细管电泳方法，以分离和定量混合的可待因，咖啡因，丁醛和阿司匹林。在第5章中，开发了毛细管电泳方法来分离和定量片剂剂型中的麻黄碱（ED），茶碱（TP）和苯巴比妥（PB）.;在第6章中，开发了胶束电动色谱（MEKC）方法在商业片剂剂型中同时分析对乙酰氨基酚（AP），咖啡因（CF）和丁比尔（BB）.;在第7章中，开发了分离达卡巴嗪及其两种相关杂质5-氨基-咪唑-4羧酰胺（AIC）的方法）和2-氮杂泛黄嘌呤（AHX）的毛细管电泳和高效液相色谱法。;在第8章中，开发了一种HPLC方法，通过固相萃取在亲和素蛋白柱上测定血浆中的消旋沙利度胺。研究了可利用的抗生物素蛋白柱对选定药物的手性分离。沙利度胺，谷氨酰胺，伯氨喹，氨基谷氨酰胺，羟嗪，氯噻酮和吡啶谷氨酰胺成功地实现了对映体的基线拆分。

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  • 作者

    Haque, Ahsanul.;

  • 作者单位

    University of Georgia.;

  • 授予单位 University of Georgia.;
  • 学科 Health Sciences Pharmacology.;Chemistry Analytical.;Chemistry Pharmaceutical.
  • 学位 Ph.D.
  • 年度 1998
  • 页码 197 p.
  • 总页数 197
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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