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首页> 外文学位 >Studies on the basal kernel blight disease of barley: Pathogenesis and phylogeny of the causal agent Pseudomonas syringae pv. syringae and its biological control by antagonistic Pantoea agglomerans.
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Studies on the basal kernel blight disease of barley: Pathogenesis and phylogeny of the causal agent Pseudomonas syringae pv. syringae and its biological control by antagonistic Pantoea agglomerans.

机译：大麦基核枯萎病研究：致病因子丁香假单胞菌PV的发病机理和系统发育。拮抗泛菌团的丁香香丁香及其生物防治。

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摘要

he pathogenesis and phylogeny of Pseudomonas syringae pv. syringae (Pss), the cause of basal kernel blight of barley, and its bioiogical control by antagonistic Pantoea agglomerans Pa, (syn. Erwinia herbicola, Eh) was investigated. Scanning electron micrographs located bacteria close to stomata and bases of kernel trichomes. Light micrographs revealed Pss in intercellular spaces of aleurone and amyloplast cells. Electron micrographs demonstrated Pss in xylem vessels and cell wall degradation in association with capsule protected bacteria.;Fifty-five Pss strains, isolated from infected kernels of 12 barley cultivars were tested for tobacco hypersensitivity, pathogenicity, toxin production and carbon source utilization. Toxin production correlated low (r = 0.31; p = 0.046) with pathogenicity, but 96% of toxin-plus strains were pathogenic, supporting the importance of toxins for virulence. Nutritional analyses revealed a phenotypic variability of Pss strains with similarity coefficients between 76-100%. The intrapathovar variation of Pss populations was analyzed by RFLPs of digested DNA using XbaI, SpeI and pulsed field gel electrophoresis. Macrorestriction fingerprinting supported a heterogeneity of Pss populations (43-77% similarity).;Field experiments (1994, 1995) revealed 45-70% disease control, when Pa was applied to batley heads prior to the Pss infection window. Pa provided 80-100% disease reduction in greenhouse studies. Efficacy of Pa was affected by time and rate of application, with a single application providing control. Survival of formulated Pa was better when stored at 4 C than 22 C. Biocontrol activity was not affected by storage.;Multiple mechanisms including antibiosis, preemptive exclusion, and induced systemic resistance were involved in the Pa-Pss interaction. Antibiotics were polar, thermostable, protease insensitive, but base labile (pH
机译：丁香假单胞菌PV的发病机制和系统发育。丁香（Pss），大麦基核枯萎病的病因，及其拮抗泛菌（Patoea agglomerans Pa）（Syn.herwinia herbicola，Eh）的生物体控制作用进行了研究。扫描电子显微照片将细菌定位在靠近气孔和仁毛状体基部的细菌处。光学显微照片显示糊粉和淀粉样细胞的细胞间隙中存在Pss。电子显微照片显示木质部血管中的Pss和与被胶囊保护的细菌相关的细胞壁降解。;对从12个大麦品种的感染核中分离的55个Pss菌株进行了烟草超敏反应，致病性，毒素产生和碳源利用的测试。毒素的产生与致病性相关性较低（r = 0.31； p = 0.046），但是96％的毒素加菌株具有致病性，支持毒素对毒力的重要性。营养分析表明，Pss菌株的表型变异性相似系数在76-100％之间。使用XbaI，SpeI和脉冲场凝胶电泳通过消化的DNA的RFLP分析Pss种群的病原体内变异。宏观限制性指纹图谱支持了Pss种群的异质性（相似性为43-77％）。实地实验（1994年，1995年）显示，当在Pss感染窗口之前将Pa应用于大麦头时，疾病控制率为45-70％。 Pa在温室研究中可减少80-100％的病害。 Pa的功效受施用时间和施用率的影响，其中一个施用提供控制。配制的Pa在4 C下储存的存活期比22 C更好。生物控制活性不受储存影响。; Pa-Pss相互作用涉及多种机制，包括抗菌，先发性排斥和诱导的系统抗性。抗生素是极性的，热稳定的，对蛋白酶不敏感的，但对碱不稳定（pH

著录项

  • 作者

    Braun, Andrea.;

  • 作者单位

    Montana State University.;

  • 授予单位 Montana State University.;
  • 学科 Agriculture Plant Pathology.;Environmental Sciences.;Agriculture Agronomy.
  • 学位 Ph.D.
  • 年度 1998
  • 页码 217 p.
  • 总页数 217
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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