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首页> 外文学位 >Effects of sodium chloride on Edwardsiella ictaluri: Studies in channel catfish, fish cell lines, and bacterial cultures.
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Effects of sodium chloride on Edwardsiella ictaluri: Studies in channel catfish, fish cell lines, and bacterial cultures.

机译：氯化钠对爱德华氏菌的影响：对channel鱼，鱼类细胞系和细菌培养的研究。

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摘要

Channel catfish Ictalurus punctatus, either unacclimated or acclimated to different saltwater concentrations (up to 2000 mg/L Cl -), were exposed by immersion or intraperitoneaI injection with Edwardsiella ictaluri, the causative agent of enteric septicemia of catfish. Freshwater-acclimated catfish, exposed to E. ictaluri and then transferred to water with different salinities did not show improved resistance to the bacteria. Edwardsiella ictaluri was transmitted from injected to uninjected freshwater-acclimated catfish in cohabitation, regardless the water salinity. When all catfish in the aquarium were injected with E. ictaluri, recovery of the bacteria after 21 d was successful for 90% of the freshwater-acclimated and for only 50% of the saltwater-acclimated catfish. No conclusion could be drawn on which salinity concentration provided the best protection.;The survival of Edwardsiella ictaluri was studied in distilled water containing different concentrations of sodium chloride and calcium. Survival of the bacteria in waters with 0, 300, 3,000 and 30,000 mg/L NaCl and 0 mg/L calcium showed inconsistent results. The effect of calcium addition (approximately 50, 100 and 200 mg/L as CaCO3) on survival, did not follow a pattern. Lowest survivals where in the 30,000 mg/L NaCl, for all the calcium concentrations.;Survival of E. ictaluri in defined minimal media was negatively affected by increased sodium chloride concentration. Bacteria in media with 10 and 15 a NaCl/L failed to survive for 48 hours, while at 5 g NaCl/L E. ictaluri survived for 84 hours.;Fish cell lines were used to evaluate the cellular invasive ability of E. ictaluri in cell cultures. Adhesion, invasion and replication were tested with 5 types of established cell lines from fish. Photomicrographs demonstrated bacteria inside the cells and confocal microscopy showed bacteria in intracellular vacuoles. The presence of large numbers of bacteria in intracellular vacuoles suggests the replication of E. ictaluri inside the cells. Quantification of the number of bacteria that adhered to and invaded cells, indicated that the invasion mechanism used by E. ictaluri was probably the same regardless of host cell type. In view of this, fish cell lines could be a valuable tool to determine virulence mechanisms of E. ictaluri.
机译：通过浸没或腹膜内注射Edward鱼爱德华氏菌引起的cat鱼Ictalurus punctatus（未适应或适应不同的海水浓度（最高2000 mg / L Cl-））暴露，该病是of鱼肠道败血症的病原体。淡水适应性。鱼，暴露于埃卡塔尔大肠杆菌，然后转移到不同盐度的水中，对细菌的抵抗力没有提高。无论水的盐度如何，爱德华氏菌都可以在同居状态下从注入到未注入的淡水驯化cat鱼传播。当将水族馆中的所有cat鱼都注射了埃卡塔尔大肠杆菌后，细菌在21天后的恢复就成功地实现了90％的淡水适应性和50％的盐水适应性fish养。对于哪种盐度提供了最好的保护，尚无定论。；在含不同浓度氯化钠和钙的蒸馏水中研究了爱德华氏菌的存活。细菌在含有0、300、3,000和30,000 mg / L NaCl和0 mg / L钙的水中生存的结果不一致。钙添加（以CaCO3计约为50、100和200 mg / L）对存活率的影响没有遵循规律。在所有钙浓度下，在30,000 mg / L NaCl中的最低存活率。氯化钠浓度升高对限定的最小培养基中的肠埃希氏菌的存活产生负面影响。 NaCl / L为10和15的培养基中的细菌不能存活48小时，而NaCl / L为5 g的大肠杆菌可以存活84小时;用鱼细胞系评价大肠杆菌的细胞侵袭能力。细胞培养。粘附，入侵和复制进行了测试从鱼的5种类型的已建立的细胞系。显微照片显示细胞内的细菌，共聚焦显微镜显示细胞内液泡中的细菌。细胞内液泡中大量细菌的存在表明伊塔利酵母在细胞内复制。粘附和侵袭细胞的细菌数量的定量表明，无论宿主细胞类型如何，大肠杆菌都可能利用相同的入侵机制。鉴于此，鱼细胞系可能是确定埃卡塔尔大肠杆菌的毒力机制的有价值的工具。

著录项

  • 作者

    Silva, Rui Manuel Cabral e.;

  • 作者单位

    Auburn University.;

  • 授予单位 Auburn University.;
  • 学科 Aquatic sciences.;Animal diseases.;Cellular biology.
  • 学位 Ph.D.
  • 年度 1998
  • 页码 120 p.
  • 总页数 120
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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