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Genomic and small subunit sequence evaluations of members of the bacterial domain: Implication for microbial evolution and taxonomic assignment.

机译：细菌域成员的基因组和小亚基序列评估：对微生物进化和分类学意义。

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摘要

Four aspects of molecular sequence analysis of the Bacterial domain are presented. In the first project, 16S rDNA from 10 marine Bacillus isolates were sequenced and analyzed. Three of the isolates appear to be terrestrial contaminants, three may be terrestrial contaminants with adaptations and the remaining four appear to be likely candidates of true marine Bacillus. The second study involved the 16S rDNA sequencing of five round spore forming Bacillus species. The sequences and subsequent analysis were used to suggest a workable bacterial taxonomy based on natural phylogeny that extends beyond genus level of the heterogenous Family Bacillaceae. This is an important step in bacterial nomenclature as the exploration of the diversity of (eu)Bacteria continues and expands. The third work introduces the concept of phylogenetic mapping. In this study, a classical morphological description was mapped onto a small subunit rRNA sequence analysis of phylogeny to determine the nature of the last common ancestor to the (eu)Bacteria and to provide insight into the phylogenetic meaning of bacterial morphological evolution. A startling pattern emerged. The coccus form of bacteria appeared to represent persistent end states of the evolution of (eu)Bacteria and have evolved independently and repeatedly. This is counter to current thinking and represents a major change in our understanding of the morphology of prokaryotes. This is only one application of the power of phylogenetic mapping, and its possible relevance to the field of biological evolution is obvious. The fourth study involves genome comparisons of six publicly available prokaryotic species and the conservation of gene order shared by them. The results indicate 19 clusters whose order remains the same among the four (eu)Bacterial genomes and reflect conserved elements of coordinated gene expression that require gene proximity. Seven clusters are preserved in the Archaea as well. Many of the clusters are regulated at the RNA level suggesting that this type of regulation may have arisen very early when there may have been entities with an RNA genome.
机译：提出了细菌域分子序列分析的四个方面。在第一个项目中，对来自10个海洋芽孢杆菌分离株的16S rDNA进行了测序和分析。分离物中的三个似乎是陆地污染物，三个可能是具有适应性的陆地污染物，其余四个似乎是真正的海洋芽孢杆菌的候选者。第二项研究涉及五个形成芽孢的芽孢杆菌物种的16S rDNA测序。该序列和随后的分析被用来基于天然系统发育，提出一种可行的细菌分类学，该分类学超越了异源芽孢杆菌科的属水平。随着对（eu）细菌多样性的探索不断扩展，这是细菌命名法中的重要一步。第三项工作介绍了系统发育映射的概念。在这项研究中，将经典的形态学描述映射到系统发育的小亚基rRNA序列分析中，以确定（eu）细菌的最后共同祖先的性质，并提供细菌形态进化的系统发育意义的见解。出现了惊人的格局。细菌的球菌形式似乎代表了（eu）细菌进化的持久性终态，并且已经独立且反复地进化。这与当前的想法背道而驰，并且代表了我们对原核生物形态的理解的重大变化。这只是系统发育作图功能的一种应用，其与生物进化领域的可能关联是显而易见的。第四项研究涉及六个公开可用的原核物种的基因组比较，以及它们共享的基因顺序的保守性。结果表明19个簇，其顺序在四个（eu）细菌基因组中保持不变，并反映了需要基因邻近性的协调基因表达的保守元素。古细菌中也保存了七个簇。许多簇在RNA水平受到调控，这表明当存在具有RNA基因组的实体时，这种调控类型可能出现得很早。

著录项

  • 作者

    Siefert, Janet Louise.;

  • 作者单位

    University of Houston.;

  • 授予单位 University of Houston.;
  • 学科 Biology Molecular.; Biology Microbiology.
  • 学位 Ph.D.
  • 年度 1997
  • 页码 155 p.
  • 总页数 155
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;微生物学;
  • 关键词

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