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Crystal structure of mammalian casein kinase I exhibits basis for phosphate recognition by a protein kinase family.

机译：哺乳动物酪蛋白激酶I的晶体结构显示了蛋白激酶家族识别磷酸盐的基础。

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Casein kinase I (CKI) enzymes phosphorylate multiple proteins with diverse functions in eukaryotic organisms and may play an important regulatory role in a variety of biological processes. These protein kinases utilize ATP in a reaction forming a phosphate mono-ester that typically can be hydrolyzed by a protein phosphatase. Common features of certain substrates show that CKI activity has an unusual specificity for proteins that are already phosphorylated on an amino acid N-terminal to the site phosphorylated by CKI. Recently, cDNAs encoding several CKI enzymes have been cloned. Sequence analysis reveals that these enzymes constitute a unique family of protein kinases. While their catalytic domains have many similarities, regions outside of this domain vary in length and sequence.;To understand the molecular basis for protein phosphorylation by CKI enzymes, experiments were designed to study the three-dimensional structure of a recombinant mammalian isoform of CKI expressed in Escherichia coli. Crystals of a truncation mutant of CKI

机译：酪蛋白激酶I（CKI）酶使真核生物中具有多种功能的多种蛋白质磷酸化，并可能在多种生物学过程中发挥重要的调节作用。这些蛋白激酶在形成磷酸单酯的反应中利用ATP，该磷酸单酯通常可以被蛋白磷酸酶水解。某些底物的共同特征表明，CKI活性对已经在CKI磷酸化位点的N端氨基酸上磷酸化的蛋白质具有异常的特异性。最近，已经克隆了编码几种CKI酶的cDNA。序列分析表明，这些酶构成了独特的蛋白激酶家族。尽管它们的催化结构域具有许多相似性，但该结构域之外的区域的长度和序列却有所不同。为了了解CKI酶使蛋白质磷酸化的分子基础，设计了实验来研究表达的CKI重组哺乳动物同工型的三维结构在大肠杆菌中。 CKI截短突变体的晶体

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作者
Longenecker, Kenton Lamar.;
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Indiana University.;

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授予单位 Indiana University.;
学科 Chemistry Biochemistry.;Biophysics Medical.
学位 Ph.D.
年度 1997
页码 160 p.
总页数 160
原文格式 PDF
正文语种 eng
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专利

1. THREE-DIMENSIONAL STRUCTURE OF MAMMALIAN CASEIN KINASE I - MOLECULAR BASIS FOR PHOSPHATE RECOGNITION [J] . Longenecker KL., Hurley TD., Roach PJ. Journal of Molecular Biology . 1996,第3期

机译：哺乳动物酪蛋白激酶I的三维结构-磷酸盐识别的分子基础。
2. The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding site and key features for protein function [J] . Elsa Franco-Echevarría, Julia Sanz-Aparicio, Charles A. Brearley, The Journal of biological chemistry . 2017,第25期

机译：哺乳动物肌醇1、3、4、5、6-五磷酸2-激酶的晶体结构揭示了新的锌结合位点和蛋白质功能的关键特征
3. The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding site and key features for protein function [J] . Elsa Franco-Echevarría, Julia Sanz-Aparicio, Charles A. Brearley, The Journal of biological chemistry . 2017,第25期

机译：哺乳动物肌醇1、3、4、5、6-五磷酸2-激酶的晶体结构揭示了新的锌结合位点和蛋白质功能的关键特征
4. Extracellular matrix proteins mediate osteogenic differentiation of human mesenchymal stem cells on phosphate functionalized gels through integrin mediated focal adhesion kinase signaling [C] . Navakanth Gandavarapu, Kristi S. Anseth Annual meeting of the Society for Biomaterials . 2011

机译：细胞外基质蛋白通过整合素介导的粘着斑激酶信号传导介导人间充质干细胞在磷酸盐功能化凝胶上的成骨分化
5. On the structure and function of calcium/calmodulin activated protein kinase II: Crystal structure of the auto-inhibited kinase domain of calcium/calmodulin-dependent kinase II and small angle X-ray scattering and electron microscopic analysis of holoenzyme assembly. [D] . Rosenberg, Oren S. 2006

机译：关于钙/钙调蛋白活化蛋白激酶II的结构和功能：钙/钙调蛋白依赖性激酶II的自抑制激酶结构域的晶体结构以及小角度X射线散射和全酶组装的电子显微镜分析。
6. Crystal structure of casein kinase-1 a phosphate-directed protein kinase. [O] . R M Xu, G Carmel, R M Sweet, 1995

机译：酪蛋白激酶1（一种磷酸化蛋白激酶）的晶体结构。
7. Crystal structure of casein kinase-1, a phosphate-directed protein kinase. [O] . R.M. Xu, G. Carmel, R.M. Sweet, 1995

机译：酪蛋白激酶-1的晶体结构，一种磷酸盐定向的蛋白激酶。
8. Characterization of the Sites Phosphorylated on Tyrosine Hydroxylase by Ca(2+) and Phospholipid-Dependent Protein Kinase, Calmodulin-Dependent Multiprotein Kinase and Cyclic AMP-Dependent Protein Kinase [R] . Vulliet, P. R., Woodgett, J. R., Ferrari, S., 1985

机译：通过Ca（2+）和磷脂依赖性蛋白激酶，钙调蛋白依赖性多蛋白激酶和环amp依赖性蛋白激酶在酪氨酸羟化酶上磷酸化位点的表征

Crystal structure of mammalian casein kinase I exhibits basis for phosphate recognition by a protein kinase family.

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