• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Effects of aging and exercise mode on serum growth hormone activity during and after exercise: Disparity between immunoassay and bioassay
【24h】

Effects of aging and exercise mode on serum growth hormone activity during and after exercise: Disparity between immunoassay and bioassay

机译：衰老和运动方式对运动前后血清生长激素活性的影响：免疫测定与生物测定之间的差异

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To examine the effect of aging and exercise mode on serum immunoreactive growth hormone (IGH) concentration vs. bioactive growth hormone (BGH) concentration during and after exercise, nine younger and eight older active women (ages, mean $pm$ 1 SEM: 23.7 $pm$ 1.0 and 61.6 $pm$ 1.3 yrs, respectively) performed acute bouts of both aerobic exercise (AE) and heavy resistance exercise (HRE). Exercise lasted 45 min and consisted of cycling at 70% of peak aerobic capacity or total-body weight training at 10-repetition maximum resistances. Venous serum samples obtained every 5 min during and for 30 min after exercise were pooled for analysis. Determinations of IGH (immunoradiometric assay) and BGH (rat tibial assay) concentrations were performed on the pooled serum before (neat) and after Sephadex fractionation into ranges of low (3-30kD), mid (30-55kD), and high ($>$55kD) molecular weight (MW). Neat serum IGH but not BGH concentration was significantly (p $leq$ 0.05) attenuated with age in both AE and HRE. Regardless of exercise mode, serum fractionation revealed an age-related shift of IGH activity from the low into the mid MW range and a shift of BGH activity from the low into both the mid and high MW ranges. In the younger women only, neat serum BGH concentration was higher during and after HRE vs. AE despite an equal IGH response between exercise modes. Although there were no differences in the fraction distribution of IGH between exercise modes in either age group, the proportion of BGH activity occurring in the mid MW range was significantly higher in HRE than in AE in the younger women. These data indicate that, during and after both AE and HRE in women, there may be an age-related shift from lower to higher MW serum GH variants resulting in a reduced serum GH immunoreactivity without a concomitant reduction in serum GH bioactivity. Furthermore, younger women may exhibit a higher serum BGH concentration during and after HRE vs. AE due to either (1) the presence in serum of a 30-55kD potentiating factor in response to HRE or (2) the presence in serum of a 30-55kD inhibitory factor in response to AE.
机译：为了研究衰老和运动方式对运动中和运动后血清免疫反应性生长激素（IGH）浓度与生物活性生长激素（BGH）浓度的影响，研究对象为9名年轻和8名活跃女性（年龄，平均$ pm $ 1 SEM：23.7） $ pm $ 1.0岁和61.6 $ pm $ 1.3岁）分别进行了有氧运动（AE）和重阻力运动（HRE）的急性发作。运动持续了45分钟，包括以有氧运动峰值能力的70％骑自行车或以最大阻力10次重复进行总体重训练。收集运动期间和运动后每5分钟获取的静脉血清样品进行分析。在Sephadex分级分离之前（纯净）和之后（低至（3-30kD），中（30-55kD）和高（$$）范围内对合并血清进行IGH（免疫放射测定）和BGH（大鼠胫骨测定）浓度的测定> $ 55kD）的分子量（MW）。在AE和HRE中，随着年龄的增长，纯净的血清IGH而非BGH浓度显着降低（p $ leq $ 0.05）。不论运动模式如何，血清分馏均显示出与年龄相关的IGH活性从低到中MW范围的变化和BGH活性从低到中MW和高MW的范围的变化。仅在年轻女性中，尽管运动模式之间的IGH响应相同，但HRE期间和之后的纯血BGH浓度要高于AE。尽管在两个年龄组中，运动模式之间的IGH分数分布均无差异，但年轻女性中，HRE发生在中分子量范围内的BGH活性比例显着高于AE。这些数据表明，在妇女的AE和HRE期间和之后，从较低MW血清GH变到较高MW可能有与年龄相关的变化，导致血清GH免疫反应性降低，而血清GH生物活性却未随之降低。此外，年轻女性在HRE期间和之后可能比AE表现出更高的血清BGH浓度，这是由于（1）血清中存在30-55kD的增强因子对HRE的反应，或者（2）血清中存在30-55kD的增强因子。 -55kD抑制因子对AE的反应。

著录项

  • 作者

    Gordon, Scott Edward.;

  • 作者单位

    The Pennsylvania State University.;

  • 授予单位 The Pennsylvania State University.;
  • 学科 Kinesiology.;Animal Physiology.
  • 学位 Ph.D.
  • 年度 1997
  • 页码 118 p.
  • 总页数 118
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号