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Molecular characterization and intracellular localization of phospholipase D in castor bean (Ricinus communis L.).

机译：蓖麻子中磷脂酶D的分子表征和细胞内定位。（Ricinus communis L.）

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The first eukaryotic phospholipase D cDNA and plant PLD gene were cloned and characterized from castor bean. The cloned PLD cDNA encodes a 92 kDa protein of 808 amino acids. Comparison of the deduced protein sequence of this cloned PLD with the N-terminal amino acid sequence of the purified PLD indicated that a 30 amino acid leader peptide precedes the mature PLD. Sequence comparison with PLD cDNA revealed that the cloned PLD gene consists of four exons and three introns, one of which interrupts the 5;Subcellular distribution of PLD in castor bean leaves and seedlings was examined with immunocytochemistry, immunoblotting and activity assays of PLD in the fractions separated with a two-phase partitioning system. These results showed that PLD was mainly located in vacuoles, and some was associated with plasma membrane and cytoplasmic matrices.;The study of the PLD gene promoter in transgenic tobacco plants also indicated that the 5

机译：从蓖麻子中克隆并鉴定了第一个真核磷脂酶D cDNA和植物PLD基因。克隆的PLD cDNA编码一个具有808个氨基酸的92 kDa蛋白。该克隆的PLD的推导的蛋白质序列与纯化的PLD的N-末端氨基酸序列的比较表明，在成熟的PLD之前有30个氨基酸的前导肽。与PLD cDNA的序列比较显示，克隆的PLD基因由四个外显子和三个内含子组成，其中一个内含子中断了5个内含子；通过免疫细胞化学，免疫印迹和级分中PLD的活性测定，检查了蓖麻子叶片和幼苗中PLD的亚细胞分布用两相分区系统分隔。这些结果表明PLD主要位于液泡中，一些与质膜和细胞质基质有关。转基因烟草植物中PLD基因启动子的研究也表明5

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作者
Xu, Liwen.;
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作者单位

Kansas State University.;

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授予单位 Kansas State University.;
学科 Molecular biology.;Plant biology.
学位 Ph.D.
年度 1996
页码 169 p.
总页数 169
原文格式 PDF
正文语种 eng
中图分类
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Molecular characterization and intracellular localization of phospholipase D in castor bean (Ricinus communis L.).

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