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首页> 外文学位 >The inositol 1,4,5-triphosphate signal transduction pathway and magnesium ion mediate lipopolysaccharide-induced exocytosis in the hemocyte of the horseshoe crab, Limulus polyphemus (L.).
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The inositol 1,4,5-triphosphate signal transduction pathway and magnesium ion mediate lipopolysaccharide-induced exocytosis in the hemocyte of the horseshoe crab, Limulus polyphemus (L.).

机译：肌醇1,4,5-三磷酸信号传导途径和镁离子介导脂多糖诱导的o马血phe（L.）的血细胞中的胞吐作用。

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摘要

The Limulus polyphemus (L.) granulocyte (GR) responds to bacterial contact by degranulating to release coagulation, agglutination and antibacterial factors that mediate an immune response. Degranulation in different invertebrate and vertebrate cells occurs through the process of exocytosis, which can be mediated by the inositol 1,4,5-triphosphate (IP{dollar}sb3{dollar}) signal transduction pathway. In this pathway, extracellular ligands, such as neurohormone analogs and bacterial lipopolysaccharide, bind to surface receptors that are linked to guanine nucleotide-binding protein (G-protein), which activate phospholipase C (PLC) to produce inositol 1,4,5-triphosphate (IP{dollar}sb3{dollar}) and diacyglycerol (DAG). IP{dollar}sb3{dollar} diffuses through the cytoplasm and binds to receptors (IP{dollar}sb3{dollar}R), which are located in the membranes of endoplasmic reticulum and specialized intracellular organelles, and form Ca{dollar}sp{lcub}2+{rcub}{dollar} channels to release stored Ca{dollar}sp{lcub}2+{rcub}{dollar} into the cytoplasm. This rise in cytoplasmic Ca{dollar}sp{lcub}2+{rcub}{dollar} ( (Ca{dollar}sp{lcub}2+{rcub}{dollar}) i) is involved in granule and plasma membrane fusion that occur during exocytosis. In this study I demonstrate, for the first time, that the neurohormone analogs octopamine, serotonin and carbachol induce degranulation and that LPS-induced exocytosis is mediated through IP{dollar}sb3{dollar} pathway, but that a large intracellular Mg{dollar}sp{lcub}2+{rcub}{dollar} ( (Mg{dollar}sp{lcub}2+{rcub}{dollar}) {dollar}sb{lcub}rm i{dollar}) flux occurs in addition to the characteristic (Ca{dollar}sp{lcub}2+{rcub}{dollar}) {dollar}sb{lcub}rm i{rcub}{dollar} flux. I also provide evidence to support the hypothesis that a (Mg{dollar}sp{lcub}2+{rcub}{dollar}) {dollar}sb{lcub}rm i{rcub}{dollar} flux is the key mediator in exocytosis in the GR.
机译：poly多肉（L.）粒细胞（GR）通过脱粒作用对细菌接触作出反应，以释放凝结，凝集和介导免疫反应的抗菌因子。在不同的无脊椎动物和脊椎动物细胞中，脱粒是通过胞吐作用发生的，它可以通过肌醇1,4,5-三磷酸（IP {dollar} sb3 {dollar}）信号转导途径来介导。在此途径中，细胞外配体（例如神经激素类似物和细菌脂多糖）与与鸟嘌呤核苷酸结合蛋白（G蛋白）相连的表面受体结合，后者激活磷脂酶C（PLC）以产生肌醇1,4,5-三磷酸（IP {dollar} sb3 {dollar}）和双甘油（DAG）。 IP {dollar} sb3 {dollar}通过细胞质扩散并与受体（IP {dollar} sb3 {dollar} R）结合，后者位于内质网和特定细胞内细胞器的膜中，并形成Ca {dollar} sp { lcub} 2+ {rcub} {dollar}通道将储存的Ca {dollar} sp {lcub} 2+ {rcub} {dollar}释放到细胞质中。细胞质Ca {dollar} sp {lcub} 2+ {rcub} {dollar}（（Ca {dollar} sp {lcub} 2+ {rcub} {dollar}）i）的升高涉及颗粒和质膜融合，发生在胞吐过程中。在这项研究中，我首次证明了神经激素类似物章鱼胺，5-羟色胺和卡巴胆碱可诱导脱粒，LPS诱导的胞吐作用是通过IP {dol} sb3 {dol}途径介导的，但细胞内的大量Mg {dollar} sp {lcub} 2+ {rcub} {dollar}（（Mg {dollar} sp {lcub} 2+ {rcub} {dollar}）{dolb} sb {lcub} rm i {dollar}）通量除了特征（Ca {dollar} sp {lcub} 2+ {rcub} {dollar}）{dolb} sb {lcub} rm i {rcub} {dollar}通量。我还提供了证据支持（Mg {dollar} sp {lcub} 2+ {rcub} {dollar}）{dollar} sb {lcub} rm i {rcub} {dollar通量是胞吐作用的关键介质的假说在GR中。

著录项

  • 作者

    Solon, Eric George.;

  • 作者单位

    Rutgers The State University of New Jersey - New Brunswick.;

  • 授予单位 Rutgers The State University of New Jersey - New Brunswick.;
  • 学科 Biology Molecular.; Biology Animal Physiology.
  • 学位 Ph.D.
  • 年度 1996
  • 页码 107 p.
  • 总页数 107
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;生理学;
  • 关键词

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