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首页> 外文学位 >Molecular cloning and physical mapping of bertha armyworm, Mamestra configurata, nuclear polyhedrosis virus genome and preliminary study of geographic isolates.
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Molecular cloning and physical mapping of bertha armyworm, Mamestra configurata, nuclear polyhedrosis virus genome and preliminary study of geographic isolates.

机译：伯氏夜蛾，Mamestra configurata，核多角体病毒基因组的分子克隆和物理作图以及地理分离物的初步研究。

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摘要

Bertha armyworm, Mamestra configurata (Lepidoptera: Noctuidae), is an important pest of cruciferous oilseed crops in western Canada. A nuclear polyhedrosis virus, MacoNPV, isolated from M. configurata, has demonstrated as high as 95% infection in field populations of bertha armyworm. MacoNPV isolates from different geographic areas differ in terms of their virulence to bertha armyworm. Restriction endonuclease (REN) fragment analyses show that all of the geographic isolates are closely related viruses but with some distinct REN pattern differences. Most of these geographic isolates are heterogenous mixtures of genotypes. The thesis describes the cloning and physical mapping of the 156.9 kbp genome of the MacoNPV-90/2 geographic isolate, including 112 restriction sites for six common REN, BamHI, EcoRI, HindIII, PstI, SmaI and XhoI. Twenty plaque purified strains of MacoNPV were isolated in a cultured Mamestra brassicae (Mbr) insect cell line. The EcoRI restriction patterns of these pick plaque (pp) strains fell into 10 different categories. In order to investigate the difference among these pp strains, and between these strains and the parental geographic isolates in terms of REN patterns, virulence to insect hosts, and their growth rates in insect cell lines, some of these isolates were selected for bioassays in bertha armyworm larvae and in the Mbr cell line.
机译：Bertha夜蛾，Mamestra configurata（鳞翅目：夜蛾科），是加拿大西部十字花科油料作物的重要害虫。从构树分枝杆菌中分离出来的核多角体病毒MacoNPV已在伯莎夜蛾田间种群中感染高达95％。来自不同地理区域的MacoNPV分离株对伯莎夜蛾的毒力不同。限制性核酸内切酶（REN）片段分析表明，所有地理分离株都是密切相关的病毒，但具有一些明显的REN模式差异。这些地理分离物中的大多数是基因型的异质混合物。论文描述了MacoNPV-90 / 2地理分离株的156.9 kbp基因组的克隆和物理作图，包括六个常见REN，BamHI，EcoRI，HindIII，PstI，SmaI和XhoI的112个限制性酶切位点。在培养的Mamestra芸苔属（Mbr）昆虫细胞系中分离出20个噬菌斑纯化的MacoNPV菌株。这些挑斑（pp）菌株的EcoRI限制模式分为10个不同类别。为了研究这些pp菌株之间以及这些菌株与亲本地理分离株之间在REN模式，对昆虫宿主的毒力及其在昆虫细胞系中的生长率方面的差异，选择了其中一些分离株用于伯莎的生物测定夜蛾幼虫和Mbr细胞系中的。

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  • 作者

    Li, Sheping.;

  • 作者单位

    The University of Saskatchewan (Canada).;

  • 授予单位 The University of Saskatchewan (Canada).;
  • 学科 Biology Microbiology.
  • 学位 Ph.D.
  • 年度 1996
  • 页码 140 p.
  • 总页数 140
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

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