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首页> 外文学位 >Numerical chromosome abnormalities in sperm from oligoasthenoteratozoospermic patients and fertile males.
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Numerical chromosome abnormalities in sperm from oligoasthenoteratozoospermic patients and fertile males.

机译：少精子少动精子症患者和可育男性精子的染色体异常数字。

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摘要

Artificial fertilization protocols have been developed which bypass natural barriers for reproduction. One aspect of infertility which has received little attention is the potential importance of aneuploidy in sperm. To more clearly understand the cytogenetic make-up of sperm from OAT patients, multi-probe, multi-color FISH was performed to determine aneuploidy.;The introduction of intracytoplasmic sperm injection (ICSI) has revolutionized protocols used in in vitro fertilization centers. The pregnancies resulting from ICSI suggest an increased frequency of sex chromosome aneuploidy in livebirths. Preliminary data by others' suggest that sperm tail swelling patterns following hypo-osmotic swelling (HOST) can be predictive of fertilizing ability. To clarify the relationship between sperm tail swelling patterns, aneuploidy, and fertilizing ability, we developed a technique combining HOST and FISH.;Using multi-probe, multi color FISH and developed formulae, we determined total aneuploidy in sperm from proven fertile donors and OAT patients. In controls, the total aneuploidy ranged between 4.1 and 7.7%. For OAT patients, the total aneuploidy was between 43 and 69%. Diploidy was found in 0.04% of sperm in controls and ranged between 0.4 and 9.6% in OAT patients.;To determine if sperm selection by swim-up can separate haploid from aneuploid sperm, we used three-probe, three-color FISH on sperm in the swim-up and pellet fractions. Between 35 and 52% of sperm from OAT patients in the swim-up fraction and 36 to 49% from the pellet were aneuploid. No significant difference was observed. However, the percent of diploid sperm predominantly resided within the pellet for all OAT and proven fertile donors.;We performed HOST and FISH on sperm from OAT patients undergoing ICSI. Data suggests that sperm tail swelling patterns A, B, D, E, F and G may be aneuploid in OAT patients. No aneuploidy was found in sperm with a C type tail swelling pattern in either OAT or normal controls. Diploid sperm in controls and OAT patients were only found in types A and G tail swelling patterns. Preliminary data suggests that there may be a correlation between HOST and aneuploidy for OAT patients.;Of the 30 OAT patients studied, 26 series of ICSI were performed. Sixty-eight percent of oocytes fertilized (165 embryos/242 oocytes). Following fresh embryo transfer, 1 preclinical abortion, 1 first trimester loss, and 2 term deliveries (2/26, 7.7%) occurred.;Our data show significant increases in the frequencies of diploidy, autosomal disomy, autosomal nullisomy, sex chromosome number, and total cytogenetic abnormalities in sperm from OAT patients versus controls. The data suggest that meiotic errors occur at highly elevated frequencies in the germ cells of severely affected OAT patients.
机译：已经开发出了人工受精方案，该方案绕过了繁殖的自然障碍。很少有人注意的不孕症的一个方面是非整倍性在精子中的潜在重要性。为了更清楚地了解OAT患者精子的细胞遗传组成，进行了多探针，多色FISH测定非整倍性。胞浆内精子注射（ICSI）的引入彻底改变了体外受精中心使用的方案。由ICSI引起的怀孕表明，在分娩时性染色体非整倍性的频率增加。其他人的初步数据表明，低渗性肿胀（HOST）后精子尾巴的肿胀模式可以预测受精能力。为了阐明精子尾部肿胀模式，非整倍性与受精能力之间的关系，我们开发了一种将HOST和FISH结合的技术;使用多探针，多色FISH并开发了公式，我们从经过验证的可育供体和OAT确定了精子中的总非整倍性耐心。在对照中，总非整倍性在4.1％和7.7％之间。对于OAT患者，总非整倍性在43％至69％之间。在对照组中，有0.04％的精子有二倍体，在OAT患者中介于0.4％和9.6％之间；为了确定通过泳动选择精子是否可以将单倍体与非整倍性精子分开，我们在精子上使用了三探针，三色FISH在浮游和颗粒部分。在上清部分中，OAT患者的精子中有35％至52％是非整倍体，而在沉淀中则有36％至49％。没有观察到显着差异。但是，对于所有OAT和已证实可育的供体，二倍体精子的百分比主要存在于沉淀物中。我们对接受ICSI的OAT患者的精子进行了HOST和FISH检测。数据表明，OAT患者的精子尾巴肿胀模式A，B，D，E，F和G可能是非整倍体。在OAT或正常对照组中，在具有C型尾巴肿胀模式的精子中未发现非整倍性。对照组和OAT患者的二倍体精子仅以A型和G型尾巴肿胀模式发现。初步数据表明，OAT患者的HOST与非整倍性之间可能存在相关性。在研究的30例OAT患者中，进行了26个ICSI系列检查。 68％的卵母细胞受精（165个胚胎/ 242个卵母细胞）。新鲜胚胎移植后，发生1次临床前流产，1次早孕期流产和2次足月分娩（2/26，7.7％）.;我们的数据显示二倍体，常染色体二体切开，常染色体无效切开，性染色体数目， OAT患者与对照组精子的总细胞遗传异常。数据表明，在严重受影响的OAT患者的生殖细胞中，减数分裂错误的发生频率很高。

著录项

  • 作者

    Pang, Myung-Geol.;

  • 作者单位

    Old Dominion University.;

  • 授予单位 Old Dominion University.;
  • 学科 Biology Genetics.;Health Sciences Obstetrics and Gynecology.
  • 学位 Ph.D.
  • 年度 1996
  • 页码 186 p.
  • 总页数 186
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 古生物学;
  • 关键词

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