首页> 外文学位 >Genes specific for Escherichia coli O157:H7 and E. coli O26 serotypes as molecular probes for rapid identification.

【24h】

Genes specific for Escherichia coli O157:H7 and E. coli O26 serotypes as molecular probes for rapid identification.

机译：特异于大肠杆菌O157：H7和大肠杆菌O26血清型的基因可作为快速鉴定的分子探针。

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

In this study, the O-antigen was cloned from E. coli O26 and E. coli O157 into the cosmid vector, pCosEMBL2. Screening of the cosmid library was done by Western analysis using polyclonal antisera specific to O26 and O157. SDS-PAGE was used to localize the LPS core and the O-antigen subunits from recombinant E. coli strains. Cosmid DNA from the O-Ag positive clones were used as DNA probes to study the distribution of the E. coli O157 and E. coli O26 rfb gene sequences. The DNA fragments that were identified from our cosmid clones appeared to be O-serotype specific for either E. coli O26 or E. coli O157. The information gained from the sequencing of these O-serotype specific DNA fragments will be used in the development of a PCR based method for detecting E. coli O26 and O157 serogroups.

机译：在这项研究中，将O抗原从大肠杆菌O26和大肠杆菌O157克隆到粘粒载体pCosEMBL2中。使用对O26和O157特异的多克隆抗血清，通过Western分析对粘粒文库进行筛选。 SDS-PAGE用于定位重组大肠杆菌菌株的LPS核心和O抗原亚基。使用O-Ag阳性克隆的粘粒DNA作为DNA探针，以研究大肠杆菌O157和大肠杆菌O26 rfb基因序列的分布。从我们的粘粒克隆中鉴定出的DNA片段似乎是针对O26大肠杆菌或O157大肠杆菌的O型血清型。从这些O型血清型特异性DNA片段的测序中获得的信息将用于开发基于PCR的检测大肠杆菌O26和O157血清型的方法。

著录项

作者
Schmidt, Denise Marie.;
展开▼
作者单位

Duquesne University.;

展开▼
授予单位 Duquesne University.;
学科 Microbiology.;Molecular biology.
学位 M.S.
年度 1996
页码 68 p.
总页数 68
原文格式 PDF
正文语种 eng
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. Intimin Gene (eae) Subtype-Based Real-Time PCR Strategy for Specific Detection of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 in Cattle Feces [J] . Delphine Bibbal, Estelle Loukiadis, Monique Kérourédan, Applied and Environmental Microbiology . 2014,第3期

机译：基于Intimin基因（eae）亚型的实时PCR策略，可特异性检测牛粪中产生志贺毒素的大肠杆菌血清型O157：H7，O26：H11，O103：H2，O111：H8和O145：H28
2. Intimin Gene (eae) Subtype-Based Real-Time PCR Strategy for Specific Detection of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 in Cattle Feces [J] . Delphine Bibbal, Estelle Loukiadis, Monique Kérourédan, Applied Microbiology . 2014,第3期

机译：基于Intimin基因（eae）亚型的实时PCR策略，可特异性检测牛粪中产生志贺毒素的大肠杆菌血清型O157：H7，O26：H11，O103：H2，O111：H8和O145：H28
3. Antimicrobial Resistance Profiles of Enterohemorrhagic and Enteropathogenic Escherichia coli of Serotypes O157:H7, O26:H11, O103:H2, O111:H8, O145:H28 Compared to Escherichia coli Isolated from the Same Adult Cattle [J] . Um Maryse Michele, Brugere Hubert, Kerouredan Monique, Microbial drug resistance: MDR : Mechanisms, epidemiology, and disease . 2018,第6期

机译：血清型O157：H7，O26：H11，O103：H2，O111：H8，O145：H28与与来自同一成年牛分离的大肠杆菌相比
4. Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry [C] . Clifton K. Fagerquist, Brandon R. Garbus, Katherine E. Williams, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：使用MALDI-TOF / TOF质谱法通过“自上而下”蛋白质组学的大肠杆菌O157：H7的快速鉴定
5. Measurements of fitness and intra-specific competition between commensal Escherichia coli and E. coli O157:H7 strains. [D] . Durso, Lisa Marie. 2003

机译：共生大肠杆菌和大肠杆菌O157：H7菌株之间适应性和种内竞争的测量。
6. Intimin Gene (eae) Subtype-Based Real-Time PCR Strategy for Specific Detection of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7 O26:H11 O103:H2 O111:H8 and O145:H28 in Cattle Feces [O] . Delphine Bibbal, Estelle Loukiadis, Monique Kérourédan, 2014

机译：基于Intimin基因（eae）亚型的实时PCR策略可特异性检测牛粪中产生志贺毒素的大肠杆菌血清型O157：H7O26：H11O103：H2O111：H8和O145：H28
7. Intimin gene (eae) subtypes-based real-time PCR strategy for the specific detection of Shiga toxin-producing Escherichia coli serotypes O157:H7, O26:H11, O103:H2, O111:H8 and O145:H28 in cattle feces [O] . Bibbal, Delphine, Loukiadis, Estelle, Kérourédan, Monique, 2014

机译：基于Intimin基因（eae）亚型的实时PCR策略，可特异性检测牛粪中产生志贺毒素的大肠杆菌血清型O157：H7，O26：H11，O103：H2，O111：H8和O145：H28

Genes specific for Escherichia coli O157:H7 and E. coli O26 serotypes as molecular probes for rapid identification.

摘要

著录项

相似文献

相关主题

期刊订阅