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首页> 外文学位 >Functional similarities between plant virus movement proteins and approaches to creating transgenic plants that restrict viral movement.
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Functional similarities between plant virus movement proteins and approaches to creating transgenic plants that restrict viral movement.

机译：植物病毒运动蛋白与产生限制病毒运动的转基因植物的方法之间的功能相似性。

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摘要

Many different plant viruses encode the ability to move from cell-to-cell through cellular plasmodesmatal boundaries. This function is attributed to the viral movement protein (MP). Because a great many viruses share similar cell-to-cell movement functions, engineered interference with viral movement is a mechanism by which multivirus resistance can be obtained in transgenic plants. Transgenic plants that express a tobacco mosaic tobamovirus (TMV) MP gene engineered to be dysfunctional were resistant to the infection of TMV, tobacco rattle tobravirus, peanut chlorotic streak caulimovirus, tobacco ringspot nepovirus, alfalfa mosaic alfamovirus and cucumber mosaic cucumovirus (CMV). Conversely, the expression of a functional TMV MP gene in transgenic plants enhanced symptoms and/or increased the accumulation of the same viruses. These studies contribute to the understanding of a multivirus resistance mechanism that blocks viral movement and reveal that synergistic viral infections can be caused by MPs.;While broad, the analysis of MPs and their interactions with components of the plant cell are incongruous. In an attempt to unite the broad generalizations previously made for two MPs, the subcellular localization of the TMV and CMV MPs from transgenic and infected tissue was examined using a single fractionation method. Transgenic TMV and CMV MPs were found in the cell wall subcellular fractions whereas, in infected tissue, the TMV MP was detected in the organelle fraction and the CMV MP was detected in fractions containing the detergent soluble components of the cell wall. This simple study shows common biological characteristics of the MPs, but it also resolves unique traits that contribute to the identities of the viral MPs themselves.;Finally, the complementary effects of the TMV and CMV MPs on the opposing viral genomes were examined. Transgenic plants that expressed a CMV MP gene could not complement the cell-to-cell movement of a movement-defective TMV. However, transgenic plants that expressed a TMV MP gene could complement the cell-to-cell movement of a movement-defective CMV. This study may help predict the extent to which MPs will be most effective in achieving the broadest and most effective form of resistance that blocks a movement mechanism that most viruses share.
机译：许多不同的植物病毒都编码了通过细胞质膜边界从一个细胞移动到另一个细胞的能力。此功能归因于病毒运动蛋白（MP）。因为许多病毒共享相似的细胞间移动功能，所以对病毒移动的工程干扰是一种可以在转基因植物中获得多病毒抗性的机制。表达工程改造为功能异常的烟草花叶烟草Tobamovirus（TMV）MP基因的转基因植物对TMV，烟草摇铃tobravirus，花生绿斑病花椰菜花叶病毒，烟草环斑nepovirus，紫花苜蓿花叶苜蓿病毒和黄瓜花叶Cucuovirus（CMV）的感染具有抗性。相反，在转基因植物中功能性TMV MP基因的表达增强了症状和/或增加了相同病毒的积累。这些研究有助于理解阻止病毒运动的多病毒抗性机制，并揭示MP可能引起协同病毒感染。；尽管如此，MP的分析及其与植物细胞成分的相互作用并不协调。为了统一先前对两个MP所做的概括，尝试使用单一分离方法检查来自转基因和感染组织的TMV和CMV MP的亚细胞定位。在细胞壁亚细胞部分中发现了转基因TMV和CMV MP，而在受感染的组织中，在细胞器部分中检测到了TMV MP，在含有细胞壁去污剂可溶成分的部分中检测到了CMV MP。这项简单的研究显示了MP的共同生物学特征，但它也解决了有助于病毒MP自身身份识别的独特特征。最后，研究了TMV和CMV MP对相反病毒基因组的互补作用。表达CMV MP基因的转基因植物不能补充运动缺陷型TMV的细胞间运动。但是，表达TMV MP基因的转基因植物可以补充运动缺陷性CMV的细胞间运动。这项研究可能有助于预测MP在达到最广泛和最有效的抗药性形式方面最有效的程度，这种抗药性会阻止大多数病毒共有的运动机制。

著录项

  • 作者

    Cooper, Bret David.;

  • 作者单位

    University of California, Riverside.;

  • 授予单位 University of California, Riverside.;
  • 学科 Biology Molecular.;Agriculture Plant Pathology.;Biology Microbiology.
  • 学位 Ph.D.
  • 年度 1995
  • 页码 112 p.
  • 总页数 112
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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