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首页> 外文学位 >Construction of genetic linkage maps and locations of disease resistant and plant architectural loci in common beans using random amplified polymorphic DNA (RAPD) markers.
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Construction of genetic linkage maps and locations of disease resistant and plant architectural loci in common beans using random amplified polymorphic DNA (RAPD) markers.

机译：使用随机扩增的多态性DNA（RAPD）标记构建普通豆中的遗传连锁图以及抗病和植物结构位点的位置。

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摘要

Common bacterial blight (CBB), web blight (WB), and rust, incited by the bacterial pathogen Xanthomonas campestris pv. phaseoli (Smith) Dye (Xcp), fungal pathogens Thanatephorus cucumeris (Frank) Donk (Tc) and Uromyces appendiculatus (Pers.:Pers) Unger (Ua), respectively, are important diseases in Phaseolus vulgaris L. The objectives of the research were: to construct linkage maps using RAPD markers in P. vulgaris recombinant inbred (RI) lines derived from the cross 'PC-50' x XAN-159 to locate CBB resistance loci (QTLs) of different plant parts to different Xcp strains and an abaxial leaf pubescence locus and from the cross BAC-6 x HT-7719 to locate CBB and WB resistance loci (QTLs), a specific rust resistance locus and plant architectural traits (QTLs).;Ten linkage groups using 180 RAPDs and the abaxial leaf pubescence locus were identified in 70 RI lines from the cross 'PC-50' x XAN-159. Eight linkage groups using 89 RAPDs and the rust resistance locus were identified in 128 RI lines from the cross BAC-6 x HT-7719.;Regression analysis and the interval mapping method using MAPMAKER/QTL were used to identify genomic regions involved in the genetic control of the traits. A chromosome region on linkage group six carried factors influencing reactions to two Xcp strains in different plant parts in the cross 'PC-50' x XAN-159. In the cross BAC-6 x HT-7719 a chromosome region on linkage group 9 carried factors influencing WB and CBB resistance in pods, first trifoliolate, and later developed leaves to Xcp EK-11.;This study illustrates the usefulness of RI lines populations and RAPD markers for mapping loci for complex traits such as CBB and WB resistance and plant architectural traits. It suggests that even though a complex of genes is associated with resistance to Xcp of different plant parts in common beans, one major gene was found to control a significant proportion of the phenotypic variation for resistance to different strains in different plant organs.
机译：细菌病原体Xanthomonas campestris pv引起的常见细菌性疫病（CBB），网纹病（WB）和铁锈。菜豆（Smith）染料（Xcp），真菌病原菌黄瓜枯草杆菌（Frank）唐克（Tc）和阑尾乌拉菌（Peromys appendiculatus）（Pers.:Pers）Unger（Ua）分别是菜豆中的重要疾病。研究的目的是：使用RAPD标记在源自“ PC-50” x XAN-159杂交的寻常重组毕赤酵母近交系（RI）中构建连锁图，以定位不同植物部位对不同Xcp菌株和背面的CBB抗性基因座（QTL）叶片的青春期基因座，并从交叉BAC-6 x HT-7719处定位CBB和WB抗性基因座（QTL），特定的抗锈基因座和植物结构特征（QTL）。；使用180个RAPD和十个背面叶片青春期的十个连锁基团在来自十字“ PC-50” x XAN-159的70条RI品系中鉴定了基因座。在来自BAC-6 x HT-7719交叉的128条RI品系中鉴定出八个连锁群，分别使用89个RAPD和抗锈基因座。;使用回归分析和使用MAPMAKER / QTL的区间作图法来鉴定参与遗传的基因组区域控制特质。连锁群6上的一个染色体区域携带影响在交叉“ PC-50” x XAN-159中不同植物部分中的两个Xcp菌株的反应的因子。在杂交BAC-6 x HT-7719中，连锁组9上的一个染色体区域携带有影响豆荚中WB和CBB抗性的因子，首先是三叶小叶，后来发育到Xcp EK-11。和RAPD标记，用于定位基因座的复杂性状，例如CBB和WB抗性以及植物结构性状。这表明，即使复杂的基因与普通豆类不同部位对Xcp的抗性相关，也发现一个主要基因控制了很大比例的表型变异，以抵抗不同植物器官中不同菌株的抗性。

著录项

  • 作者

    Jung, Geunhwa.;

  • 作者单位

    The University of Nebraska - Lincoln.;

  • 授予单位 The University of Nebraska - Lincoln.;
  • 学科 Agriculture Plant Pathology.;Biology Genetics.;Biology Molecular.
  • 学位 Ph.D.
  • 年度 1995
  • 页码 106 p.
  • 总页数 106
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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