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首页> 外文学位 >Two-photon laser scanning microscopy, microlithography, and multi-layered optical data storage.
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Two-photon laser scanning microscopy, microlithography, and multi-layered optical data storage.

机译：双光子激光扫描显微镜，微光刻和多层光学数据存储。

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摘要

When a visible wavelength beam from an ultrashort pulse mode-locked laser is focused by high numerical aperture optics within a UV absorbing medium, quadratic dependence of two-photon absorption on the intensity of the exciting radiation confines fluorescence excitation and induced photochemistry predominantly within a focal volume of less than one cubic micrometer. This tiny focal volume provides an exceptionally well defined light tool for laser scanning fluorescence microscopy, direct writing photolithography, and multi-layered optical data storage.; Optical transfer functions are calculated for selected single- and two-photon microscopes. Optical sectioning or background rejection of the two-photon microscopes, even when using a wide field detector, is seen to be comparable to that of the single-photon confocal microscope. Furthermore, the two-photon instrument restricts photobleaching to the focal plane, facilitating the collection of image depth series for three dimensional image reconstructions. Finally, chromatic aberration with UV excited fluorophores, a problem for single-photon confocal microscopes, is relieved by two-photon excitation using visible wavelengths.; Two-photon excited scanning photopolymerization and photolithography using a sharply focused single beam allows microfabrication of complex structures not possible with single-photon excitation. Results showing half micron wide trenches of very high aspect ratio, and resist structures with undercutting edges, all produced with only a single development step, demonstrate the potential utility of two-photon excitation in microfabrication.; Finally, unprecedented high density ({dollar}>{dollar}10{dollar}sp{lcub}12{rcub}{dollar} bits/cm{dollar}sp3{dollar}) optical recording of digital information in a multi-layered, three dimensional format is demonstrated. Information written as submicron volume elements of increased refractive index within a photopolymer medium is read out with sufficient axial resolution by differential interference contrast microscopy.; Photobleaching, saturation, and intersystem crossing--all problems for conventional single photon laser scanning microscopy--are also measured for two important fluorophores.
机译：当来自超短脉冲锁模激光器的可见波长光束被紫外吸收介质中的高数值孔径光学器件聚焦时，双光子吸收对激发辐射强度的二次依赖性将荧光激发和诱导的光化学作用主要局限在焦点内体积小于一立方微米。这种微小的焦点体积为激光扫描荧光显微镜，直接写入光刻和多层光学数据存储提供了一个非常明确定义的照明工具。对于选定的单光子和双光子显微镜，计算光学传递函数。即使使用宽视野检测器，双光子显微镜的光学切片或背景抑制也可与单光子共聚焦显微镜相媲美。此外，双光子仪器将光漂白限制在焦平面上，从而有助于为三维图像重建收集图像深度序列。最后，使用可见光波长的双光子激发可以缓解单光子共聚焦显微镜的紫外线激发荧光团的色差。使用锐利聚焦的单光束的双光子激发扫描光聚合和光刻技术可以实现单光子激发不可能实现的复杂结构的微细加工。结果表明，只有极短的显影步骤就可以生产出具有极高纵横比的半微米宽的沟槽，以及带有底切边缘的抗蚀剂结构，所有这些都仅需一个显影步骤即可完成，证明了双光子激发在微细加工中的潜在实用性。最后，以前所未有的高密度（{dollar}> {dollar} 10 {dollar} sp {lcub} 12 {rcub} {dollar} bits / cm {dollar} sp3 {dollar}）光学记录多层数字信息，演示了三维格式。通过差动干涉对比显微镜以足够的轴向分辨率读出写为光聚合物介质内折射率增加的亚微米体积元素的信息。光漂白，饱和度和系统间交叉-传统的单光子激光扫描显微镜的所有问题-也可以通过两种重要的荧光团进行测量。

著录项

  • 作者

    Strickler, James H.;

  • 作者单位

    Cornell University.;

  • 授予单位 Cornell University.;
  • 学科 Physics Optics.
  • 学位 Ph.D.
  • 年度 1994
  • 页码 155 p.
  • 总页数 155
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 光学;
  • 关键词

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