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首页> 外文学位 >Effects of osmotic stress on anthocyanins and other metabolites in Eucalyptus tereticornis cell cultures.
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Effects of osmotic stress on anthocyanins and other metabolites in Eucalyptus tereticornis cell cultures.

机译：渗透胁迫对桉树桉树细胞培养物中花色苷和其他代谢产物的影响。

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摘要

The purpose of this study was to use anthocyanin-producing cell cultures of Eucalyptus tereticornis to study the relationship between osmotic stress and anthocyanin synthesis. The initial hypothesis was that the synthesis of anthocyanins in response to osmotic stress is due to a decline in chlorophyll levels which in turn causes an increase in anthocyanin levels to provide protection of cell components from harmful radiation.;This study not only showed the utility of cell cultures for studying stress-related metabolism but it also showed various metabolic changes that occur in plant cell cultures of E. tereticornis in response to osmotic stress. Initial studies using callus cultures were conducted to evaluate the effect of light intensity, the type and concentration of auxin, and mannitol-induced osmotic stress on growth and anthocyanin synthesis. Additional osmotic stress experiments were conducted using suspension cultures to study the effect of mannitol-induced osmotic stress not only on anthocyanin synthesis but also on chlorophyll, soluble sugar and free amino acid levels.;Based on the results of this work several important conclusions are made about cellular responses to osmotic stress in E. tereticornis cultures. First, evidence is presented that cell cultures of E. tereticornis respond to increasing osmotic stress with an increase in anthocyanins levels. However, indirect evidence is also presented which indicates that this response may indeed be dependent on chlorophyll levels in the cells. Second, levels of sucrose were shown to decrease in response to increasing osmotic stress, possibly indicating that sucrose, which accumulates in the cells, may be used as a carbon source for stress-related metabolism. Third, results showed that E. tereticornis cultures respond similarly to other plants by increasing levels of free amino acids in response to osmotic stress. Results also showed that the most dramatic increase occurred in the level of proline while the level of phenylalanine, a precursor to anthocyanins, did not show any significant response to osmotic stress.
机译：这项研究的目的是使用产于桉树的花色素苷的细胞培养物研究渗透压与花色素苷合成之间的关系。最初的假设是花青素对渗透压的响应是由于叶绿素水平的下降而导致的，这反过来又导致花青素水平的增加，从而保护细胞组分免受有害辐射的侵害。细胞培养物用于研究与压力有关的代谢，但它也显示出在变应性大肠杆菌的植物细胞培养物中响应渗透胁迫而发生的各种代谢变化。进行了使用愈伤组织培养的初步研究，以评估光强度，生长素的类型和浓度以及甘露醇诱导的渗透胁迫对生长和花色苷合成的影响。使用悬浮培养进行了另外的渗透胁迫实验，以研究甘露醇诱导的渗透胁迫不仅对花色苷合成，而且对叶绿素，可溶性糖和游离氨基酸水平的影响。基于这项工作的结果，得出了几个重要结论。关于E. tereticornis培养物中细胞对渗透压的反应。首先，有证据表明，戊酸大肠杆菌的细胞培养物随着花色苷水平的增加而对增加的渗透压作出反应。但是，还提供了间接证据，表明该反应确实可能取决于细胞中的叶绿素水平。其次，显示出蔗糖水平响应于增加的渗透压而降低，这可能表明积累在细胞中的蔗糖可以用作应激相关代谢的碳源。第三，结果表明，戊酸大肠埃希菌培养物通过增加对渗透胁迫的响应的游离氨基酸水平，对其他植物的反应相似。结果还表明，脯氨酸的含量增加最为明显，而花青素的前体苯丙氨酸的含量则对渗透压没有任何明显的反应。

著录项

  • 作者

    Castillon, Javier.;

  • 作者单位

    Texas A&M University.;

  • 授予单位 Texas A&M University.;
  • 学科 Agriculture Plant Culture.;Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 1994
  • 页码 82 p.
  • 总页数 82
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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