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Phylogenetic investigations in the genus Coprinus based on morphological and molecular characters.

机译：基于形态和分子特征的鬼伞属的系统发育研究。

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Phylogenetic relationships among species of Coprinus and closely related taxa were studied using molecular and morphological data. Four studies were carried out. In the first study 7 taxa in Coprinus, 5 potential sister group taxa from the Bolbitiaceae and Strophariaceae, and 2 secotioid taxa were investigated. Restriction analysis was carried out on the nuclear gene encoding the large ribosomal subunit RNA and the internally transcribed spacer region II. A total of 153 sites were mapped of which 54 were phylogenetically informative. Six equally-most parsimonious networks were resolved using cladistic methods. In the second study relationships among 24 isolates from section Lanatuli were investigated. Isolates were separated into 6 intersterility groups. Sequence data was generated for the 24 isolates and for 2 outgroup taxa, Agrocybe praecox and Psathyrella gracilis, from the nuclear gene encoding the large ribosomal subunit RNA. Using a strict consensus tree of 20 equally-most parsimonious trees, isolates could be grouped into 6 terminal clades each supported by bootstrap proportions greater than 90%. These clades could be ranked as species on the basis of their delimitation through intersterility studies. In the third study phylogenetic relationships were investigated in 47 species of Coprinus, 17 additional species from the families Coprinaceae, Strophariaceae, Bolbitiaceae, and Agaricaceae, and two secotioid taxa believed to be closely related to Coprinus. Sequence analysis was carried out on portions of the nuclear gene coding for the large ribosomal subunit RNA. A total of 1,360 sites were sequenced across 7 divergent domains. A total of 302 phylogenetically informative characters were found. Ninety-eight percent of the average divergence between taxa was located within the divergent domains. Domains D2 and D8 were the most divergent while domains D7 and D10 were the least divergent. A strict consensus integration of the 2 equally-most parsimonious trees demonstrates that the genus Coprinus is made up of 3 clades. In the fourth study phylogenetic relationships in the genus Coprinus were investigated using morphological and molecular characters. Parsimony-based phylogenetic analysis of 47 morphological characters, using a subset of the taxa from the third study, produced 11895 equally-most parsimonious trees 284 steps long. A consensus tree had little resolution but did show a relationship between Leucocoprinus, section Comati, and the 2 secotioid taxa as in the 1st and 3rd studies. The 47 morphological and the 1360 sequence characters from the 3rd study were combined and analyzed. Three equally-most parsimonious trees each 1800 steps long were produced. Relationships were very similar to those from a molecular analysis alone. Marked changes were seen, however, in bootstrap proportions. (Abstract shortened by UMI.)
机译：利用分子和形态学数据研究了鸡腿菇和紧密相关的类群之间的亲缘关系。进行了四项研究。在第一项研究中，研究了鸡腿菇中的7个分类单元，5个潜在的姊妹类单元，分别来自Bolbitiaceae和Strophariaceae，以及2个secotioid类单元。对编码大核糖体亚基RNA和内部转录的间隔区II的核基因进行了限制性分析。总共绘制了153个位点，其中54个在系统发育上提供了信息。使用cladistic方法解析了六个同样最简单的网络。在第二项研究中，研究了来自Lanatuli切片的24个分离株之间的关系。分离物分为6个不育组。从编码大核糖体亚基RNA的核基因产生了24个分离株和2个外群分类群，农杆菌和沙门氏菌的序列数据。使用严格的共识树，其中包含20个最等同的简约树，可以将分离株分为6个末端进化枝，每个进化枝受大于90％的引导程序支持。这些枝条可以通过不育性研究根据它们的划界被列为物种。在第三项研究中，研究了鸡腿菇属的47种，科pr科，菊科，茄科和琼脂科的另外17种，以及两个与鸡腿藻密切相关的类拟类群。对编码大核糖体亚基RNA的核基因部分进行了序列分析。共有1,360个位点在7个不同的域上测序。共发现302个系统发育信息特征。分类单元之间平均差异的百分之九十八位于差异域内。域D2和D8差异最大，而域D7和D10差异最小。对2个最均等的简约树进行严格的共识整合表明，鸡腿菇属由3个进化枝组成。在第四项研究中，使用形态学和分子特征研究了鸡腿菇属的系统发生关系。基于简约性的系统发育分析，对47个形态特征进行了分析，使用了第三项研究中的一个分类单元，产生了11895个等长的简约树，长284步。共识树几乎没有分辨率，但确实显示出Leucocoprinus，Comati切片和第2和第3项研究中的2个类古生物类群之间的关系。对第三项研究的47个形态学特征和1360个序列特征进行了组合和分析。产生了三棵最同等的简约树，每棵树长1800步。关系与单独进行分子分析时的关系非常相似。但是，自举比例出现了明显的变化。 （摘要由UMI缩短。）

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