首页> 外文学位 >Genetics and gene flow of organophosphate resistance in three predatory mites, Amblyseius andersoni Chant, Typhlodromus pyri Scheuten and Metaseiulus occidentalis Nesbitt (Acarina: Phytoseiidae), in Oregon.
【24h】

Genetics and gene flow of organophosphate resistance in three predatory mites, Amblyseius andersoni Chant, Typhlodromus pyri Scheuten and Metaseiulus occidentalis Nesbitt (Acarina: Phytoseiidae), in Oregon.

机译:俄勒冈州的三只捕食性螨,安德森A虫(Amblyseius andersoni Chant),鼠伤寒伤寒沙门氏菌(Typhlodromus pyri Scheuten)和西方球墨螨(Metaseiulus occidentalis Nesbitt)(螨类:Phytoseiidae)的遗传学和基因流。

获取原文
获取原文并翻译 | 示例

摘要

Genetics, gene flow, and distribution of pesticide resistance traits were examined for organophosphate (OP) resistance in three beneficial phytoseiid mites. Levels and genetics of OP resistance in Amblyseius andersoni were examined first. Laboratory strains from Italy and Oregon, USA, were compared in susceptibility to insecticides used in western Oregon fruit crops. The Italian strain was 80-100 times more resistant to the OPs azinphosmethyl, diazinon, malathion, and phosalone, as well as carbaryl, a carbamate. Significant differences were not found between strains for endosulfan or fenvalerate. Using backcross analysis, response of F{dollar}sb1{dollar} hybrids to azinphosmethyl indicated OP resistance was semidominant. Through novel statistical analysis, backcross of F{dollar}sb1{dollar} to parent strains revealed resistance was polygenic, with at least two loci. Reciprocal crosses demonstrated the presence of maternal effects, with increased variation associated with progeny of Oregon females.; In the next set of experiments, electrophoresis of allozymes was used to estimate gene flow for Typhlodromus pyri. Ten populations from two apple growing valleys of Oregon were compared. Subpopulations were collected from in and around commercial apple orchards. Four loci unaffected by pesticide use were examined. F{dollar}sb{lcub}rm ST{rcub}{dollar} was calculated at 0.115, and Nm as 2.08. No allelic patterns could be discerned for populations among or within valleys; however, more variation was present for mite populations within valleys than between them. Some inbreeding was found within populations. While from dispersal studies one would conclude T. pyri is non-dispersive, allozymic analysis indicates there is moderate gene flow.; Factors affecting OP resistance distribution in T. pyri and Metaseiulus occidentalis were examined. A diagnostic concentration of azinphosmethyl was used to determine OP resistance frequencies for populations of each species, collected in and near commercial apple orchards in two valleys. OP resistance in T. pyri populations was localized: mites from 10 m or more outside orchards were OP susceptible, while those within orchards were resistant. This indicated limited gene flow. All M. occidentalis populations were resistant, indicating a regional resistance pattern and high gene flow. Factors which were not significant in the distribution of OP resistance were: valley, degree of orchard isolation, host plant, and seasonality.
机译:遗传学,基因流和农药抗性性状的分布检查了三个有益的植物螨的有机磷(OP)抗性。首先检查了安德森A虫中OP抗性的水平和遗传。比较了意大利和美国俄勒冈州的实验室菌株对俄勒冈西部水果作物中使用的杀虫剂的敏感性。意大利菌株对OPs的谷硫磷,甲基二嗪农,马拉硫磷和phosalone以及氨基甲酸酯西维因的抗性要高80-100倍。硫丹或芬戊酸酯的菌株之间未发现显着差异。使用回交分析,F {dollar} sb1 {dollar}杂种对谷硫磷的反应表明OP抗性居于主导地位。通过新颖的统计分析,F {dollar} sb1 {dollar}与亲本菌株的回交显示抗性是多基因的,具有至少两个基因座。相互的杂交证明存在母体效应,与俄勒冈州女性后代相关的变异增加。在下一组实验中,同工酶的电泳被用于估计鼠伤寒沙门氏菌的基因流。比较了俄勒冈州两个苹果种植谷地的十个种群。从商业苹果园内和周围收集亚群。检查了四个不受农药使用影响的位点。 F {dollar} sb {lcub} rm ST {rcub} {dollar}的计算值为0.115,Nm为2.08。对于山谷之间或山谷内的种群,无法识别等位基因模式;但是,山谷内螨虫种群的变化多于它们之间的变异。在种群中发现了一些近交。从分散研究中可以得出,T。pyri是非分散的,但同功酶分析表明存在适度的基因流动。检验了影响T. pyri和Metaseiulus occidentalis的OP阻力分布的因素。诊断浓度的谷硫磷用于确定每个物种种群的OP抗性频率,该频率收集在两个山谷的商业苹果园内及附近。 T. pyri种群中的OP抗性是局部的:来自10 m或以上果园的螨对OP敏感,而果园内的螨具有抗性。这表明基因流受限。所有西方欧美分枝杆菌种群均具有抗药性,表明区域抗药性模式和高基因流量。在OP抗性分布中不重要的因素是:谷,果园隔离度,寄主植物和季节性。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号