DNA sequence analysis of a Helicobacter pylori plasmid fragment.

机译：幽门螺杆菌质粒片段的DNA序列分析。

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The purpose of this study is to determine the nucleotide sequence of a 700 bp plasmid DNA fragment isolated from the gastric pathogen, Helicobacter pylori. The DNA sequence has been analyzed for homology to other plasmid nucleic acid and amino acid sequence.; The 700 bp fragment was subjected to dideoxy chain termination DNA sequence and computer database analysis using DNAsis and PC-Gene program. One open reading frame was identified and the deduced polypeptide was 228 amino acids with predicted molecular weight of 27623. Three prokaryotic genes with short regions of DNA homology were found. However, no ribosome binding site and transcription termination sequences were revealed in the sequence. The 700 bp fragment must be part of an unknown protein. Sequencing the neighboring fragments may provide better identification of this plasmid-encoded protein.

机译：这项研究的目的是确定分离自胃病原体幽门螺杆菌的700 bp质粒DNA片段的核苷酸序列。已经分析了DNA序列与其他质粒核酸和氨基酸序列的同源性。用DNAsis和PC-Gene程序对700bp片段进行双脱氧链终止DNA序列和计算机数据库分析。鉴定出一个开放阅读框，推导的多肽为228个氨基酸，预测分子量为27623。发现了三个具有短DNA同源性的原核基因。但是，序列中没有揭示核糖体结合位点和转录终止序列。 700 bp的片段必须是未知蛋白质的一部分。对相邻片段进行测序可以更好地鉴定该质粒编码的蛋白。

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作者
Chen, Jie.;
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作者单位

Texas Woman's University.;

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授予单位 Texas Woman's University.;
学科 Biology Microbiology.; Biology General.; Biology Molecular.
学位 M.S.
年度 1993
页码 58 p.
总页数 58
原文格式 PDF
正文语种 eng
中图分类微生物学;普通生物学;分子遗传学;
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1. Sequence and gene expression analyses of plasmid pHPM8 from Helicobacter pylori reveal the presence of two operons with putative roles in plasmid replication and antibiotic activity. [J] . Quinones M, Knesek JE, McIntire SA Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 2001,第3期

机译：幽门螺杆菌质粒pHPM8的序列和基因表达分析表明存在两个操纵子，在质粒复制和抗生素活性中具有推定作用。
2. Genetic organization and conjugal plasmid DNA transfer of pHP69, a plasmid from a Korean isolate of Helicobacter pylori [J] . Joo J.-S., Song J.-Y., Baik S.-C., The journal of microbiology . 2012,第6期

机译：幽门螺杆菌的韩国分离株pHP69的遗传组织和接合质粒DNA转移
3. Multilocus sequence typing of DNA from faecal specimens for the analysis of intra-familial transmission of Helicobacter pylori [J] . Takako Osaki, Masumi Okuda, Junko Ueda, Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland . 2013,第5期

机译：粪便标本DNA的多基因座序列分型，用于分析幽门螺杆菌的家族内传播
4. Monitoring bacterial processes by Fourier transform infrared spectroscopy: Helicobacter pylori drug inactivation and plasmid bioproduction in recombinant Escherichia coli cultures [C] . Scholz Teresa, Lopes Vitor V., Calado Cecilia R. C. 1st Portuguese Meeting in Bioengineering . 2011

机译：通过傅里叶变换红外光谱监测细菌过程：幽门螺杆菌药物失活和重组大肠杆菌培养物中的质粒生物生产
5. Analysis of mucin oligosaccharides in the rhesus monkey stomach after Helicobacter pylori infection and transcriptional modulation of outer membrane protein expression in Helicobacter pylori [D] . Cooke, Cara Lea 2008

机译：幽门螺杆菌感染后恒河猴胃粘蛋白寡糖分析及幽门螺杆菌外膜蛋白表达的转录调控
6. Note: Analysis of vacA cagA and IS605 Genotypes and Those Determined by PCR Amplification of DNA between Repetitive Sequences of Helicobacter pylori Strains Isolated from Patients with Nonulcer Dyspepsia or Mucosa-Associated Lymphoid Tissue Lymphoma [O] . Nathalie E. M. van Doorn, Ferry Namavar, Leen-Jan van Doorn, 1999

机译：注意：从非溃疡性消化不良或与黏膜相关的淋巴样组织淋巴瘤患者中分离出的幽门螺杆菌重复序列之间的vacAcagA和IS605基因型分析以及通过DNA的PCR扩增确定的基因分析
7. Tracing clonality of Helicobacter pylori infecting family members from analysis of DNA sequences of three housekeeping genes (ureI, atpA and ahpC), deduced amino acid sequences, and pathogenicity-associated markers (cagA and vacA) [O] . Robert J. Owen, Jacqueline Xerry 2003

机译：幽灵杆菌的克隆性来自分析三个内政基因（Urei，ATPA和AHPC）的DNA序列分析，推导氨基酸序列和致病性相关标记（CAGA和VACA）

DNA sequence analysis of a Helicobacter pylori plasmid fragment.

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