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首页> 外文学位 >Quantitative trait loci analysis of tuber traits in diploid potato (Solanum spp.).
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Quantitative trait loci analysis of tuber traits in diploid potato (Solanum spp.).

机译：二倍体马铃薯（Solanum spp。）块茎性状的数量性状基因座分析。

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One breeding method for potato (Solanum tuberosum subsp. tuberosum) is using wild species. This method could be more efficient if the introgression of genes from these species were monitored with molecular markers. Furthermore, the use of molecular markers allows the dissection of quantitative traits into discrete genetic factors. The objective of this research was to perform quantitative trait loci (QTL) analysis on two tuber traits in potato: specific gravity and dormancy. Two diploid populations were constructed from heterozygous self-incompatible parents. These two populations, TRP 132 (127 individuals) and TRP133 (110 individuals) have a common maternal parent and combine genomes of Solanum tuberosum (haploid), S. chacoense, and S. phureja. A preliminary analysis using isozymes was performed. QTLs were determined by one-way analyses of variances for each locus by trait combination (P {dollar}<{dollar} 0.05). Epistatic interactions were detected through two-way analyses of variance. Further studies focused on TRP133, which was characterized for 10 isozyme loci, 44 RFLPs and 63 RAPDs. Eighty-seven loci segregating from the female parent were utilized to construct a linkage map comprising 10 of the 12 chromosomes in the genome. For dormancy, 6 QTLs were identified that explained 57.5% of the phenotypic variation for the trait. Specific gravity was evaluated in 3 environments. QTLs were mapped separately for each location and in combination. A total of 10 QTLs on six chromosomes were identified. The numbers and effects of QTLs detected varied across environments, and they explained from 39% to 45% of the phenotypic variation for the trait. Using the average data a multilocus model was developed. This gives consistent results when tested across environments, and may be valuable for marker-assisted selection. This research developed the basic methodology for QTL analysis in potato which is now available for future studies with other traits and germplasms.
机译：马铃薯（马铃薯（Solanum tuberosum subsp。tuberosum））的一种育种方法是使用野生物种。如果使用分子标记监测这些物种的基因渗入，则该方法可能更有效。此外，使用分子标记可以将数量性状分解为离散的遗传因素。这项研究的目的是对马铃薯的两个块茎性状：比重和休眠进行定量性状位点（QTL）分析。从杂合的自我不相容亲本构建了两个二倍体种群。这两个种群，TRP 132（127个个体）和TRP133（110个个体）有一个共同的母本，并结合了马铃薯（单倍体），链球菌和金黄色葡萄球菌的基因组。使用同工酶进行了初步分析。通过对每个基因座的性状组合进行方差单向分析来确定QTL（P {dollar} <{dollar} 0.05）。通过方差的双向分析检测上位相互作用。进一步的研究集中在TRP133，其特征是10个同工酶基因座，44个RFLP和63个RAPD。从雌性亲本中分离出的八十七个基因座被用于构建一个连锁图谱，该图谱包括基因组中12条染色体中的10条。对于休眠，确定了6个QTL，这些QTL解释了该性状的表型变异的57.5％。在3种环境中评估比重。针对每个位置分别组合绘制了QTL。共鉴定出六个染色体上的10个QTL。检测到的QTL的数量和影响在不同环境中各不相同，它们解释了该性状表型变异的39％至45％。使用平均数据，开发了多基因座模型。在跨环境进行测试时，这将提供一致的结果，并且可能对标记辅助选择很有用。这项研究开发了马铃薯中QTL分析的基本方法，现在可用于其他性状和种质的未来研究。

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