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Microbial degradation of polycyclic aromatic hydrocarbon and cyanide in soils from manufactured gas plant sites.

机译：人造煤气厂场地土壤中多环芳烃和氰化物的微生物降解。

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摘要

The microbial clean-up of cyanide and polycyclic aromatic hydrocarbon (PAH) in soils from manufactured gas plant (MGP) sites is the subject of this study. Cyanide was examined for its inhibition on microbial PAH degradation by an MGP-soil isolate identified as a strain of Pseudomonas aeruginosa by classical differential methods as well as 16S rRNA oligonucleotide probes. A strong cyanide-degrading Bacillus pumilus (ATCC #7061) strain was used for facilitating cyanide degradation thereby enhancing PAH biodegradation in this soil. This research has validated cyanide interference with the PAH degrader and shown that adding Bacillus pumilus accomplishes the removal of cyanide which subsequently allows Pseudomonas aeruginosa to metabolize PAHs.; In addition to the biodegradation of cyanide and lower ring numbered PAHs, the microbial degradation of 4-ring polycyclic aromatic hydrocarbons (PAHs) by using a mixed culture obtained from another former coal tar contaminated site was also studied. The rate of biotransformation and the abiotic loss due to volatilization were monitored. The 3-ring PAH used in this project was phenanthrene and the 4-ring PAHs used were fluoranthene and pyrene. The results showed that volatilization loss of naphthalene in the control system was substantial while volatilization of higher molecular weight PAH compounds (fluoranthene and pyrene) was negligible. The biodegradation rates of phenanthrene, fluoranthene and pyrene are 6.56, 1.59 and 0.82 mg/L/day, respectively or 65.6, 15.9, 8.2 mg/gram of cells/day assuming 100 mg cells/L in the system. This study indicates that biodegradation of 3- and 4-ring PAHs by mixed cultures obtained from PAH contaminated sites is very promising.; These studies will contribute to the understanding of PAH and cyanide removal from MGP sites. It provides the essential information for the design of a bioremediation project to reclaim the unusable land that was contaminated through the previous coal gasification process.
机译：本研究的主题是微生物净化天然气厂（MGP）站点土壤中的氰化物和多环芳烃（PAH）。通过经典差分方法以及16S rRNA寡核苷酸探针，通过鉴定为铜绿假单胞菌菌株的MGP土壤分离物检测了氰化物对微生物PAH降解的抑制作用。使用强氰化物降解短小芽孢杆菌（ATCC＃7061）菌株促进氰化物降解，从而增强土壤中PAH的生物降解。这项研究已验证了氰化物对PAH降解物的干扰，并表明添加短小芽孢杆菌可完成氰化物的去除，随后铜绿假单胞菌可代谢PAHs。除了氰化物和低级环编号的PAHs的生物降解之外，还研究了通过使用从另一个以前的煤焦油污染部位获得的混合培养物对4环多环芳烃（PAHs）的微生物降解作用。监测生物转化率和由于挥发引起的非生物损失。本项目中使用的3环PAH是菲，使用的4环PAH是荧蒽和pyr。结果表明，控制系统中萘的挥发损失很大，而较高分子量的PAH化合物（荧蒽和pyr）的挥发可以忽略不计。假设系统中有100 mg细胞/ L，菲，荧蒽和pyr的生物降解率分别为6.56、1.59和0.82 mg / L / day或65.6、15.9、8.2 mg / g细胞/天。这项研究表明，从PAH污染部位获得的混合培养物对3环和4环PAH的生物降解非常有前景。这些研究将有助于理解从MGP位点去除PAH和氰化物。它为设计一个生物修复项目提供了重要信息，该项目旨在回收通过先前的煤气化过程被污染的不可用土地。

著录项

  • 作者

    Ho, YiFong.;

  • 作者单位

    Rensselaer Polytechnic Institute.;

  • 授予单位 Rensselaer Polytechnic Institute.;
  • 学科 Environmental Sciences.; Engineering Civil.
  • 学位 Ph.D.
  • 年度 1993
  • 页码 183 p.
  • 总页数 183
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境科学基础理论;建筑科学;
  • 关键词

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