• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Molecular-marker-mediated dissection of quantitative inheritance in maize (Zea mays L.): Characterization of favorable exotic factors and comparisons of statistical methodologies and marker technologies.
【24h】

Molecular-marker-mediated dissection of quantitative inheritance in maize (Zea mays L.): Characterization of favorable exotic factors and comparisons of statistical methodologies and marker technologies.

机译：玉米（Zea mays L.）的分子标记介导的数量遗传剖析：有利的外来因子的表征以及统计方法和标记技术的比较。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Exotic maize germplasm, shown to be useful for developing improved temperate cultivars, has remained little used partly because of many inherent shortcomings. Molecular markers allow the identification of genetic factors underlying the expression of agronomic traits and can also facilitate their introgression from donor (exotic) into recipient (US) germplasm. However, cost and time involved in molecular marker analyses are still limiting factors for most breeding applications.;Five F;Three molecular marker technologies, RFLPs based on chemiluminescence, RFLPs based on radioactivity, and RAPDs, were compared in terms of cost and time efficiencies using simulations of maize genotyping projects. RAPDs were the most cost-efficient strategy when small sample sizes were to be analyzed. RFLPs were the least expensive methods for large numbers of individuals. Relative time efficiencies were comparable to cost efficiencies, except that RFLPs based on radioactivity always required more time than RFLPs based on chemiluminescence. The choice of a molecular marker strategy should be determined by the type and amount of information sought, the availability of markers or previous information for the species, and the ability to integrate the generated information into a general database.
机译：已显示外来玉米种质可用于开发改良的温带品种，但由于许多固有缺点，仍很少使用。分子标记可以鉴定农艺性状表达基础的遗传因素，也可以促进它们从供体（外来）向受体（US）种质渗入。然而，分子标记物分析涉及的成本和时间仍然是大多数育种应用的限制因素。五，三种分子标记物技术，基于化学发光的RFLP，基于放射性的RFLP和RAPD在成本和时间效率方面进行了比较使用玉米基因分型计划的模拟。当要分析小样本量时，RAPD是最具成本效益的策略。对于许多人而言，RFLP是最便宜的方法。相对时间效率可与成本效率相媲美，只是基于放射性的RFLP始终比基于化学发光的RFLP需要更多的时间。分子标记策略的选择应取决于所需信息的类型和数量，标记的可用性或物种的先前信息以及将生成的信息整合到通用数据库中的能力。

著录项

  • 作者

    Ragot, Michel.;

  • 作者单位

    North Carolina State University.;

  • 授予单位 North Carolina State University.;
  • 学科 Biology Genetics.
  • 学位 Ph.D.
  • 年度 1992
  • 页码 151 p.
  • 总页数 151
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号