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Genetic and biological differences among Japanese encephalitis virus strains from various regions of Asia.

机译：亚洲不同地区的日本脑炎病毒株之间的遗传和生物学差异。

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摘要

Fifty-eight strains of Japanese encephalitis (JE) virus from a variety of geographic areas in Asia were examined by primer-extension sequencing of the RNA templates. Two hundred-forty nucleotides from the C and pre-M gene regions were selected for study, since this region provided sufficient information for determining genetic relationships among the virus isolates. Using 12% divergence as a cutoff point for virus relationships, the 58 isolates fell into four distinct genotypic groups. One genotypic group consisted of JE virus isolates from northern Thailand and Cambodia. A second group was comprised of isolates from southern Thailand, Malaysia, Sarawak and Indonesia. Isolates from Japan, China, Taiwan, the Philippines, Sri Lanka, India and Nepal made up a third group. Several isolates from Indonesia formed a distinct fourth genotype. Strains belonging to the second and fourth genotypic groups were from areas where JE virus infection is common but encephalitis is rare. In contrast, strains from areas in which JE epidemics have occurred fell into the first and third genotypes. In an attempt to find biological virulence markers which might correlate with these genotypic variations, in vivo and in vitro systems were tested. Although individual differences among JE virus strains in plaque morphology and mouse virulence were found, no consistent biological patterns could be demonstrated among the various genotypes.
机译：通过对RNA模板进行引物延伸测序，检测了来自亚洲各个地区的58株日本脑炎（JE）病毒。从C和pre-M基因区域选择了240个核苷酸进行研究，因为该区域提供了足够的信息来确定病毒分离株之间的遗传关系。使用12％的差异作为病毒关系的临界点，将58个分离株分为四个不同的基因型组。一个基因型组包括来自泰国北部和柬埔寨的JE病毒分离株。第二类包括来自泰国南部，马来西亚，砂拉越和印度尼西亚的分离株。来自日本，中国，台湾，菲律宾，斯里兰卡，印度和尼泊尔的隔离群构成第三组。来自印度尼西亚的几种分离物形成了独特的第四基因型。属于第二和第四基因型组的菌株来自流行性乙型脑炎病毒感染但脑炎罕见的地区。相反，来自发生乙脑流行病地区的菌株属于第一和第三基因型。为了寻找可能与这些基因型变异相关的生物毒力标记，测试了体内和体外系统。尽管发现JE病毒株之间在噬菌斑形态和小鼠毒性方面存在个体差异，但在各种基因型之间均未显示出一致的生物学模式。

著录项

  • 作者

    Chen, Woan-Ru Justin.;

  • 作者单位

    Yale University.;

  • 授予单位 Yale University.;
  • 学科 Biology Microbiology.;Health Sciences Public Health.
  • 学位 Ph.D.
  • 年度 1992
  • 页码 134 p.
  • 总页数 134
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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