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首页> 外文学位 >A role for thrombospondin in peripheral and central nervous system regeneration.
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A role for thrombospondin in peripheral and central nervous system regeneration.

机译：血小板反应蛋白在外周和中枢神经系统再生中的作用。

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摘要

Factors present in the extracellular environment are crucial to the success of nerve regeneration. One recently identified extracellular matrix molecule, thrombospondin (TSP), has the properties of a factor potentially involved in nerve regeneration. For instance, TSP is developmentally regulated in the mouse nervous system during periods of neuronal migration and axonal outgrowth, and it supports neurite outgrowth in vitro.;This work shows that TSP is present in nervous systems capable of nerve regeneration, has an expression pattern associated with active nerve regeneration, and promotes nerve regeneration in vivo. The pattern of expression of TSP was correlated with the regenerative potential of regions of mouse, goldfish, xenopus, and newt nervous systems, indicating that TSP is localized in a position relevant to nerve regeneration. Furthermore, changes in expression of TSP correlate with successful regeneration. First, following crush or transection injury to the mouse sciatic nerve, TSP expression increased distal to the trauma site, and was dramatically enriched in close association with regenerating neurites. After successful reinnervation, TSP returned to control levels. Second, following optic nerve crush in the goldfish, the level of TSP increased in the connective tissue septae along which regenerating axons grew. However, following crush injury of the mouse optic nerve, TSP was present in discreet clumps associated with macrophages but not in a pattern conducive to nerve regeneration. TSP also enhanced regeneration in an experimental paradigm. When polyethylene tubes filled with TSP, anti-TSP, laminin, or PBS were grafted into the mouse sciatic nerve, nerve cables regenerating through TSP filled grafts were consistently thicker and contained more axons than other groups. Addition of anti-TSP antibodies to the tubes significantly decreased Schwann cell migration.;Based on these results, TSP joins a small group of extracellular molecules, which are capable of supporting neurite outgrowth in vitro and whose expression corresponds with active nerve regeneration in vivo. The presence of TSP promotes nerve regeneration into polyethylene grafts, and addition of anti-TSP antibodies greatly inhibits nerve regeneration. Consistent with its expression pattern during neuronal development and its ability to support neurite outgrowth in vitro, TSP appears to play an important role in nerve regeneration in vivo.
机译：存在于细胞外环境中的因素对于神经再生的成功至关重要。最近发现的一种细胞外基质分子，血小板反应蛋白（TSP）具有潜在参与神经再生的因子的特性。例如，TSP在神经元迁移和轴突生长期间在小鼠神经系统中受到发育调节，并且在体外支持神经突生长。;这项工作表明TSP存在于能够神经再生的神经系统中，具有相关的表达模式具有活跃的神经再生，并在体内促进神经再生。 TSP的表达方式与小鼠，金鱼，非洲爪蟾和new神经系统区域的再生潜力相关，表明TSP定位在与神经再生有关的位置。此外，TSP表达的变化与成功再生有关。首先，在小鼠坐骨神经受到挤压或横切损伤后，TSP表达在创伤部位的远端增加，并显着富集，与再生神经突紧密相关。成功进行神经支配后，TSP恢复到控制水平。其次，在金鱼中视神经受到挤压之后，结缔组织隔中的TSP含量增加，再生轴突沿其生长。然而，在小鼠视神经被挤压伤后，TSP存在于与巨噬细胞相关的离散团块中，但没有以有助于神经再生的方式存在。 TSP在实验范式中也增强了再生。当将填充有TSP，抗TSP，层粘连蛋白或PBS的聚乙烯管移植到小鼠坐骨神经中时，通过填充有TSP的移植物再生的神经电缆始终较厚，并且轴突的数量超过其他组。在管中加入抗TSP抗体可显着降低雪旺细胞的迁移。基于这些结果，TSP加入了一小组细胞外分子，它们能够在体外支持神经突生长，其表达与体内活跃的神经再生相对应。 TSP的存在会促进神经再生为聚乙烯移植物，而抗TSP抗体的加入会大大抑制神经再生。 TSP在神经元发育过程中的表达模式及其在体外支持神经突向外生长的能力与之相一致，在体内神经再生中似乎发挥着重要作用。

著录项

  • 作者

    Hoffman, John Randall.;

  • 作者单位

    University of Michigan.;

  • 授予单位 University of Michigan.;
  • 学科 Neurosciences.;Cellular biology.
  • 学位 Ph.D.
  • 年度 1992
  • 页码 135 p.
  • 总页数 135
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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