• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >A biochemical analysis of the nematocyst structural proteins from Aiptasia pallida (Cnidaria: Anthozoa).
【24h】

A biochemical analysis of the nematocyst structural proteins from Aiptasia pallida (Cnidaria: Anthozoa).

机译：生化分析来自拟南芥（Aiptasia pallida）的线虫胞囊结构蛋白（Cndaria：Anthozoa）。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

One feature common to all Cnidarians is the production of highly complex secretion products known as cnidae. It has been shown that all cnidae develop within a Golgi-derived vesicle of a cnidocyte. This vesicle, called the nematocyst primordium, at some time must contain all the molecules and ions that make up the structural material of the cnidae, the components of the venom, and other intracapsular components such as the cofactors or the enzymes that might be involved with vesicular biochemical modification relating to activity and assembly. Past studies have concentrated on ultrastructural changes during development, but little or no work has been done on the biochemical events underlying these processes. Only recently has work been done on the biochemistry of mature nematocyst capsules and threads.; This project addresses questions pertaining to the biochemistry of the nematocyst capsule wall and thread proteins. Early work on the chemistry of nematocyst capsules found the wall and tubule of the microbasic mastigophore nematocyst from Aiptasia pallida to be composed of a single collagen-like, 31.8 kd protein linked by disulfide bonds (Blanquet, 1966; Fishman and Levy, 1967; Phelan and Blanquet, 1985). Using Aiptasia, this thesis demonstrated, however, several disulfide bound, imino acid- and glycine-rich proteins, ranging in M{dollar}sb{lcub}rm r{rcub}{dollar} from 20 to 217 kd, with the major protein found at 40 kd. Similar analyses of structural proteins from the microbasic mastigophores of Metridium senile and the isorhizas of Physalia physalis reveal that the major protein obtained from each is also disulfide-linked, and has a similar molecular weight. Proteins obtained from the solubilization of microbasic mastigophores from Aiptasia pallida and Metridium senile appear more similar to each other than to those of Physalia physalis isorhizas, based on molecular weight, carbohydrate and sulfhydryl content. Analysis of nematocyst capsular and thread proteins, using monoclonal antibodies prepared for this purpose, confirmed suspected differences between nematocyst capsular and thread proteins, and also highlighted intraspecific differences among cnidae. Analyses of the reoxidation of reduced preparations of nematocyst structural proteins revealed an aggregation process that suggests possible mechanisms for nematocyst assembly in vivo. A model for wall development is proposed.
机译：所有Cnidarians的共同特征之一就是生产高度复杂的分泌产品，即cnidae。已经显示所有的all科都在高尔基派生的小胞囊内发育。该囊泡有时被称为线虫囊原基，必须包含构成the科结构材料，毒液成分以及其他囊内成分（例如辅因子或可能与之相关的酶）的所有分子和离子。与活性和组装有关的水泡生化修饰。过去的研究集中在开发过程中的超微结构变化上，但是对这些过程背后的生化事件的研究很少甚至没有做。直到最近才对成熟的线虫囊胶囊和线的生物化学进行了研究。该项目解决与线虫囊壁和线蛋白的生物化学有关的问题。线虫囊胶囊化学的早期研究发现，来自淡水拟南芥的微小乳突线虫线虫囊的壁和小管由单一的类胶原蛋白，31.8 kd的蛋白质通过二硫键连接而成（Blanquet，1966； Fishman和Levy，1967； Phelan和Blanquet，1985年）。然而，本论文利用Aiptasia证实了几种二硫键结合的，富含亚氨基酸和甘氨酸的蛋白质，其主要成分为M {dollar} sb {lcub} rm r {rcub} {dollar}，范围从20 kd到217 kd。发现于40 kd。对老年Me和微酸的微乳突虫的结构蛋白进行的相似分析显示，从每种中获得的主要蛋白也是二硫键连接的，分子量相似。基于分子量，糖类和巯基含量，从拟南芥（Aiptasia pallida）和衰老的Metridium的老年麦芽肿中增溶了微生物的乳突igo获得的蛋白质彼此之间的相似性要比is浆异or的相似。使用为此目的准备的单克隆抗体对线虫囊膜和线蛋白进行分析，证实了线虫囊膜和线蛋白之间的可疑差异，并且突出了科的种内差异。减少的线虫囊结构蛋白制备物的再氧化分析显示，聚集过程提示体内线虫囊组装的可能机制。提出了一种用于墙发展的模型。

著录项

  • 作者

    Brand, David Douglass.;

  • 作者单位

    Georgetown University.;

  • 授予单位 Georgetown University.;
  • 学科 Biology Cell.; Biology Zoology.; Chemistry Biochemistry.
  • 学位 Ph.D.
  • 年度 1992
  • 页码 172 p.
  • 总页数 172
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;动物学;生物化学;
  • 关键词

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号