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The determination of lithium and other trace elements in blood serum by ICP-MS

机译：ICP-MS测定血清中锂和其他微量元素

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摘要

Inductively coupled plasma mass spectrometry (ICP-MS) is a powerful tool for elemental analysis and has been used to find the composition of many materials in recent years. This thesis deals with the analytical utility of ICP-MS for the determination of endogenous levels of Li in serum Factors such as the scanned mass range and the dwell time were found to significantly affect the instrument response. Under optimum conditions for Li the serum matrix was found to cause limited non-spectroscopic interferences. Elemental recombinations of the major electrolytes in the matrix did not affect Li determination. However, several biologically important elements e.g. Cr, V, and As were significantly affected by these interferences which limited the usability of the multi-element capabilities of the technique. The feasibility of the analysis of dilute serum was investigated. The results showed that the high levels of proteins in serum caused a rapid deterioration of the magnitude and the stability of the signal. Therefore, a wet digestion technique using HNO3 was devised. The method proved to be accurate and precise not only for Li but also for B, Mg, Co, Cu, Zn, Se, Br, Rb and Cs. International standard reference materials (SRM) were used in order to test the accuracy of the overall procedures. Comparable results to the certified values were obtained for all the elements studied. In addition, this work provided the first data for Li and B in two SRMs, namely, NIST bovine serum and Versieck's second generation serum. The levels of Li in the serum of healthy subjects were evaluated based on the analysis of 196 samples. The data showed that the reference value for Li in serum approximates to 0.9 ng.ml-1. To demonstrate the applicability of this work for biological studies, the levels of Li, B, Mg, Co, Cu, Zn, Se, Br, Rb and Cs were determined in the serum of patients with skin disorders. Several changes in trace element levels e.g. the elevation of Li in polymorphic light eruption were observed. Overall, this study demonstrates the suitability of ICP-MS for routine multi-element analysis of several trace elements in serum. Although matrix dependent problems can be a limiting factor, ICP-MS is likely to become the instrument of choice for routine analysis of biological materials.
机译：电感耦合等离子体质谱法（ICP-MS）是元素分析的强大工具，近年来已用于发现许多材料的成分。本论文涉及ICP-MS在测定血清中Li的内源性水平方面的分析实用性。诸如扫描质量范围和保压时间等因素均显着影响仪器响应。在锂的最佳条件下，发现血清基质引起有限的非光谱干扰。基质中主要电解质的元素重组不会影响Li的测定。但是，一些生物学上重要的元素例如Cr，V和As受这些干扰影响很大，这些干扰限制了该技术的多元素功能的可用性。研究了稀释血清分析的可行性。结果表明，血清中高水平的蛋白质引起信号强度和信号稳定性的快速下降。因此，设计了使用HNO 3的湿消化技术。该方法不仅对锂而且对硼，镁，钴，铜，锌，硒，Br，Rb和Cs都是准确而精确的。为了测试整个程序的准确性，使用了国际标准参考材料（SRM）。对于所有研究的元素，均获得了与认证值可比的结果。此外，这项工作为两个SRM（NIST牛血清和Versieck的第二代血清）中的Li和B提供了第一个数据。通过对196个样品的分析，评估了健康受试者血清中的Li水平。数据表明血清中Li的参考值约为0.9 ng.ml-1。为了证明这项工作在生物学研究中的适用性，测定了皮肤病患者血清中的Li，B，Mg，Co，Cu，Zn，Se，Br，Rb和Cs的水平。痕量元素水平的一些变化观察到锂在多态喷发中的升高。总的来说，这项研究证明了ICP-MS适用于血清中多种微量元素的常规多元素分析。尽管依赖于基质的问题可能是一个限制因素，但ICP-MS可能会成为生物材料常规分析的首选工具。

著录项

  • 作者

    Abou-Shakra, Fadi R.;

  • 作者单位

    University of Surrey (United Kingdom).;

  • 授予单位 University of Surrey (United Kingdom).;
  • 学科 Analytical chemistry.
  • 学位 Ph.D.
  • 年度 1991
  • 页码 218 p.
  • 总页数 218
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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