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Preparation and application of bifunctional chelates for biological electron transfer studies and preparation of monoclonal antibodies.

机译：用于生物电子转移研究的双功能螯合物的制备和应用以及单克隆抗体的制备。

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摘要

Iodoacetyl functionality is known to specifically bind methionine 65 residue on cytochrome c. Cytochrome c interacts with cytochrome c oxidase and reductase via the region around this amino acid.;The synthesis of a bifunctional chelate derivative of EDTA with an iodoacetyl side functional group was investigated. The last step in the synthesis of this compound produces two products based on the published methods. Model reactions were conducted to find conditions which produces only the desired product.;Bifunctional chelate derivative of water soluble tetrasulfonatotetraphenylporphyrin was used to generate and isolate antibodies against the metalloporphyrin CoTPPS4. Covalent binding of this metalloporphyrin to bovine serum albumin and keyhole limpet hemocyanin was accomplished by preparing the CoTPPS3C where one of the sulfonate functional groups on the phenlyporphyrin is replaced with a carboxylic acid functionality. The preparation of this metalloporphyrin is described. Several mass cultures were isolated and screened by ELISA. Mass cultures 2B5, 2B9 and 2C3 were specific for the antigen, CoTPPS3C. 2B9 mass culture was cloned and a monoclonal antibody was isolated which proved to be highly specific against CoTPPS3C.;In the following section the electrochemistry of TPPS4 and CoTPPS4 was investigated in aqueous solution at pH range of 1-14 by using various electrodes. Following, the electrochemistry of this metalloporphyrin was investigated by using several modifiers and promoters.;The electrochemistry of CoTPPS4 and TPPS4 in DMF/TEAP was investigated. The Diffusion coefficient and heterogeneous rate constant of CoTPPS4 and TPPS4 were calculated by using a working planar gold electrode. Finally the electrochemistry of CoTPPS4 in DMF/H;The role of tetramethylpyridiniumporphyrin (TMPy) and its Co
机译：已知碘乙酰基官能团能特异性结合细胞色素c上的蛋氨酸65残基。细胞色素c通过该氨基酸周围的区域与细胞色素c氧化酶和还原酶相互作用。研究了具有碘乙酰基侧官能团的EDTA的双功能螯合衍生物的合成。该化合物合成的最后一步根据公开的方法产生了两种产物。进行模型反应以发现仅产生所需产物的条件。水溶性四磺酰氨基四苯基卟啉的双官能螯合衍生物用于产生和分离针对金属卟啉CoTPPS4的抗体。该金属卟啉与牛血清白蛋白和钥孔戚血蓝蛋白的共价结合是通过制备CoTPPS3C来实现的，在该CoTPPS3C中，苯基卟啉上的磺酸盐官能团之一被羧酸官能团取代。描述了该金属卟啉的制备。分离出几种大众培养物并通过ELISA筛选。大众培养2B5、2B9和2C3对抗原CoTPPS3C具有特异性。克隆了2B9大规模培养物，并分离出了对CoTPPS3C具有高度特异性的单克隆抗体。在以下部分中，通过使用各种电极在pH范围为1-14的水溶液中研究TPPS4和CoTPPS4的电化学。随后，通过使用几种修饰剂和促进剂研究了该金属卟啉的电化学性质。;研究了CoTPPS4和TPPS4在DMF / TEAP中的电化学性质。通过使用工作的平面金电极计算CoTPPS4和TPPS4的扩散系数和异质速率常数。最后介绍了CoTPPS4在DMF / H中的电化学；四甲基吡啶卟啉（TMPy）及其钴的作用

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  • 作者

    Emad, Mehrzad.;

  • 作者单位

    University of Florida.;

  • 授予单位 University of Florida.;
  • 学科 Organic chemistry.;Biochemistry.;Analytical chemistry.;Immunology.
  • 学位 Ph.D.
  • 年度 1991
  • 页码 160 p.
  • 总页数 160
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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