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A structure/function study of Escherichia coli and Anabaena 7120 glutamine synthetase.

机译：大肠杆菌和鱼腥藻7120谷氨酰胺合成酶的结构/功能研究。

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The chemical and kinetic mechanism of low (LA) and high adenylylated (HA) E. coli and Anabaena 7120 glutamine synthetase (GS) was studied. Anabaena 7120 GS exhibited lower biosynthetic activity and higher transferase activity compared to LA E. coli GS. Their Michaelis constants were similar, except for ammonia.;In the presence of L-methionine-(S)-sulfoximine (MSOX), L-methionine sulfodiimine (MSOXD), and L-phosphinothricin the continuous wave (cw) EPR spectra of Mn (II) bound at the n;In the presence of chemically synthesized MSOXP the n;Pulsed EPR was used to study the n;Inhibition by AMP and CTP of Anabaena 7120 GS was studied kinetically. AMP inhibited by binding at the ATP active site and an AMP allosteric site. CTP did not inhibit.

机译：研究了低（LA）和高腺苷酸（HA）大肠杆菌和鱼腥藻7120谷氨酰胺合成酶（GS）的化学和动力学机理。与LA大肠杆菌GS相比，鱼腥藻7120 GS表现出较低的生物合成活性和较高的转移酶活性。除了氨以外，它们的Michaelis常数相似。;在存在L-蛋氨酸-（S）-亚磺酰亚胺（MSOX），L-蛋氨酸磺二亚胺（MSOXD）和L-膦丝菌素的情况下，Mn的连续波EPR谱（II）结合在n上；在化学合成的MSOXP存在下，使用n脉冲EPR研究n；动力学研究Anabaena 7120 GS的AMP和CTP抑制作用。 AMP被ATP活性位点和AMP变构位点结合而抑制。 CTP没有抑制。

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作者
Rocca, Lorenzo Andrew.;
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作者单位

The Pennsylvania State University.;

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授予单位 The Pennsylvania State University.;
学科 Chemistry Biochemistry.;Chemistry Organic.;Biophysics General.
学位 Ph.D.
年度 1990
页码 264 p.
总页数 264
原文格式 PDF
正文语种 eng
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1. In vivo functional characterization of the Escherichia coli ammonium channel AmtB: evidence for metabolic coupling of AmtB to glutamine synthetase. [J] . Javelle A, Thomas G, Marini AM, The Biochemical Journal . 2005,第Pta1期

机译：大肠杆菌铵通道AmtB的体内功能表征：AmtB与谷氨酰胺合成酶代谢偶联的证据。
2. Molecular characterization of two novel proteins All1122 and Alr0750 of Anabaena PCC 7120 conferring tolerance to multiple abiotic stresses in Escherichia coli [J] . Sen Sonia, Rai Ruchi, Chatterjee Antra, Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function . 2019,第期

机译：两种新蛋白All1122和Anabaena PCC 7120的分子表征Anabaena PCC 7120赋予大肠杆菌在大肠杆菌中多种非生物胁迫的耐受性
3. Decoding the role of hypothetical protein All3255 of Anabaena PCC7120 in heavy metal stress management in Escherichia coli [J] . Singh Prashant Kumar, Tang Mengmeng, Kumar Sudhir, Archives of microbiology . 2018,第3期

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4. A SPECIAL ISOFORM OF THE RIESKE-FES PROTEIN IS INVOLVED IN HETEROCYST FUNCTION OF THE CYANOBACTERIUM ANABAENA SP. PCC 7120 [C] . Matthias Arnold, Gunter Hauska, Iris Maldener International Congress of Photosynthesis . 2005

机译：RIESKE-FES蛋白的特殊同种型参与了anabaena SP的杂物杂物功能。 PCC 7120.
5. An investigation to explore the role of the C-terminal helical thong in the stability of the Escherichia coli glutamine synthetase dodecamer and to explore the minimum quaternary structure of glutamine synthetase needed for the regulatory adenylylation reaction. [D] . Stoffel, Robert H., III. 1994

机译：探究C末端螺旋丁字裤在大肠杆菌谷氨酰胺合成酶十二肽稳定性中的作用，并探讨调节腺苷酸化反应所需的谷氨酰胺合成酶的最小四级结构。
6. The patA gene product, which contains a region similar to CheY of Escherichia coli, controls heterocyst pattern formation in the cyanobacterium Anabaena 7120. [O] . J Liang, L Scappino, R Haselkorn 2018

机译：patA基因产物包含一个与大肠杆菌CheY相似的区域，它控制蓝藻鱼腥藻7120中的异囊模式形成。
7. Isolation of the Gene for the B12-Dependent Ribonucleotide Reductase from Anabaena sp. Strain PCC 7120 and Expression in Escherichia coli [O] . Gleason, Florence K., Olszewski, Neil E. 2002

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A structure/function study of Escherichia coli and Anabaena 7120 glutamine synthetase.

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