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首页> 外文学位 >CHARACTERIZATION OF THE SPORE COAT INVOLVED IN THE GERMINATION OF BACILLUS CEREUS T SPORES.
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CHARACTERIZATION OF THE SPORE COAT INVOLVED IN THE GERMINATION OF BACILLUS CEREUS T SPORES.

机译：芽孢杆菌T孢子萌发中孢子皮的鉴定。

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摘要

Bacillus cereus T spores, sporulated on Fortified Nutrient Agar, were extracted of the spore coat and outer membrane by 0.5% sodium dodecyl sulfate-100 mM dithiothreitol (SDS-DTT) in 0.1 M sodium chloride at pH 10.5 (coat-defective spores). The spore coat and/or outer membrane removal was confirmed by examination of normal and coat-defective spores for their ability to partition in n -hexadecane and transmission electron microscopy. Coat-defective spores germinated slowly and to a lesser extent than normal spores in 10 mM plus 5 mM adenosine as determined by decrease in absorbance and release of dipicolinic acid and ;The extract showed a general proteolytic, trypsin-, and lysozyme-like activities. Germination was inhibited by tosyl-arginine methyl ester and L-1-tosylamide-phenylethyl chloromethyl ketone indicating that trypsin-like activity may be partially involved in the germination of B. cereus T spores.;When coat-defective spores were heat activated with the dialyzed (12,000-
机译：用pH值为10.5的0.1 M氯化钠中的0.5％十二烷基硫酸钠-100 mM二硫苏糖醇（SDS-DTT）提取孢子在强化营养琼脂上形成的蜡状芽孢杆菌T孢子。通过检查正常的和有缺陷的孢子在正十六烷中的分配能力和透射电子显微镜检查，证实了孢子的被膜和/或外膜的去除。皮毛缺陷芽孢在10 mM加5 mM腺苷中发芽的速度比正常孢子慢，但发芽程度较小，这是通过吸收和降低二吡啶甲酸的释放确定的；和提取物表现出一般的蛋白水解，胰蛋白酶和溶菌酶活性。甲苯磺酰基精氨酸甲酯和L-1-甲苯磺酰胺-苯乙基氯甲基酮抑制了发芽，表明类胰蛋白酶活性可能与蜡状芽孢杆菌的T孢子萌发有关。透析（12,000-

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  • 作者

    KUTIMA, PHILIP MUSEVE.;

  • 作者单位

    North Carolina State University.;

  • 授予单位 North Carolina State University.;
  • 学科 Agriculture Food Science and Technology.
  • 学位 Ph.D.
  • 年度 1987
  • 页码 162 p.
  • 总页数 162
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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