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MATURATION OF THE MURINE RESPIRATORY EPITHELIUM: PATTERNS OF PRECURSOR UPTAKE AND TEMPORAL BEHAVIOUR OF INCORPORATED MATERIALS.

机译：呼吸道呼吸道上皮的成熟：前体摄取的模式和掺入材料的时间行为。

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摘要

The pattern of incorporation of various extracellular matrix material precursors into extracellular matrix materials of developing lung tissues was investigated at stages corresponding to glandular, fetal saccular and postnatal saccular periods. Of the precursors investigated, labeling patterns with ;Most of these materials at each stage were N-linked, as judged by sensitivity to tunicamycin homologue B2. Treatment of fetal tissues with tunicamycin did not appear to inhibit branching morphogenesis. However, the expansion of terminal bud lumina seen with cultured glandular stage tissue was inhibited, indicating that these N-linked materials may play a directive role in the thinning of the terminal epithelium in the transition from glandular to saccular stages. The location of these materials during stages when lung tissues are functional may also indicate a role at these stages, perhaps as structural stabilizers for the respiratory epithelium. (Abstract shortened with permission of author.).;At the glandular stage, label was preferentially incorporated into cells of the terminal buds, but not over future airways. At the fetal saccular stage, label was preferentially incorporated into future respiratory regions, where it was localized primarily to the apical region of the epithelia. Labeling patterns at the postnatal saccular stage were comparable to those seen at the fetal saccular stage. Label incorporated at glandular stages was largely retained and terminal buds formed expanded saccular-like structures. At fetal saccular stages, label was also largely retained, but the lumina of saccules collapsed with culture. Label incorporated at postnatal saccular stages was rapidly turned over, and saccular lumina also collapsed. Clearly, the response of the tissues with regards to fate of the labeled materials under conditions of chase and of the tissue itself to culture conditions was different at each stage. These labeled materials were large, with apparent molecular weights in the range of 800-1,000 kDa.
机译：在与腺体，胎儿囊和出生后囊期相对应的阶段研究了将各种细胞外基质材料前体掺入到发育中的肺组织的细胞外基质材料中的模式。根据对衣霉素同系物B2的敏感性判断，在所研究的前体中，用标记的模式大多数是在每个阶段都是N-连接的。用衣霉素处理胎儿组织似乎没有抑制分支形态发生。然而，在培养的腺期组织中看到的末端芽管腔的扩张被抑制，表明这些N-连接的物质可能在从腺体到囊期的转变中在末端上皮的变薄中起指导作用。这些材料在肺组织起作用的阶段中的位置也可能表明在这些阶段中的作用，可能作为呼吸道上皮的结构稳定剂。 （摘要在作者许可下缩短。）；在腺体阶段，标签优先掺入末端芽的细胞中，但不掺入未来的气道中。在胎囊期，标记物优先掺入未来的呼吸区域，在该区域主要定位于上皮的顶端区域。产后囊期的标记模式与胎儿囊期的标记模式相当。在腺体阶段合并的标签大部分保留下来，末端芽形成膨胀的囊状结构。在胎囊期，标记物也大部分保留，但囊泡腔随着培养而塌陷。在产后囊期合并的标签迅速翻转，囊腔也塌陷。显然，组织在追逐条件下对标记材料的命运的反应以及组织本身对培养条件的反应在每个阶段都是不同的。这些标记的材料很大，表观分子量在800-1,000 kDa范围内。

著录项

  • 作者

    WEBSTER, EARL HAROLD, JR.;

  • 作者单位

    Temple University.;

  • 授予单位 Temple University.;
  • 学科 Biology.
  • 学位 Ph.D.
  • 年度 1987
  • 页码 159 p.
  • 总页数 159
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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