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Purification of immunoglobulins, their bonding onto latex particles and the development of latex agglutination test for mouse immunoglobulin-G for monoclonal antibody assessment.

机译：免疫球蛋白的纯化，它们与乳胶颗粒的键合以及用于小鼠免疫球蛋白-G的乳胶凝集试验的发展，用于单克隆抗体评估。

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Sheep anti-mouse antibodies (SaMIg) and mouse antibodies have been purified by saturated ammonium sulfate salt precipitation and DEAE-Cellulose column chromatography. They were further characterised by Disc gel electrophoresis and Ouchterlony gel methods.;The sensitivity of the latex agglutination test with purified mouse IgG was about 0.005 to 1 ;The sheep anti-mouse IgG's were adsorbed onto the latex particles with various particle sizes and surface chemical groups. Passive and covalent adsorption methods have been achieved using various buffer systems and condensing reagents (spacers) respectively. The effect of pH on the adsorption of IgG's onto latex particles was also studied and the ideal pH, temperature, latex particle size and nature, purity of the antibody etc., for successful coating of the antibody have been defined.

机译：羊抗小鼠抗体（SaMIg）和小鼠抗体已通过饱和硫酸铵盐沉淀和DEAE-纤维素柱色谱法纯化。通过圆盘凝胶电泳和Ouchterlony凝胶方法对其进行了进一步表征。;纯化的小鼠IgG的乳胶凝集试验的灵敏度约为0.005比1;绵羊抗小鼠IgG被吸附在具有不同粒径和表面化学性质的乳胶颗粒上组。分别使用各种缓冲系统和缩合试剂（垫片）已实现了被动和共价吸附方法。还研究了pH对IgG在乳胶颗粒上的吸附的影响，并且定义了用于成功包被抗体的理想pH，温度，乳胶颗粒大小和性质，抗体纯度等。

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作者
Sompuram, Seshi Reddy.;
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作者单位

Boston College.;

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授予单位 Boston College.;
学科 Health Sciences Immunology.
学位 M.S.
年度 1987
页码 152 p.
总页数 152
原文格式 PDF
正文语种 eng
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1. Evaluation of a monoclonal antibody-based latex agglutination test for diagnosis of cryptococcosis: comparison with two tests using polyclonal antibodies. [J] . A Temstet, P Roux, J L Poirot, Journal of Clinical Microbiology . 1992,第10期

机译：评估基于单克隆抗体的乳胶凝集试验以诊断隐球菌病：与使用多克隆抗体的两种试验进行比较。
2. Evaluation of a monoclonal antibody-based latex agglutination test for diagnosis of cryptococcosis: comparison with two tests using polyclonal antibodies. [J] . A Temstet, P Roux, J L Poirot, Journal of Clinical Microbiology . 1992,第10期

机译：评估基于单克隆抗体的乳胶凝集试验以诊断隐球菌病：与使用多克隆抗体的两种试验进行比较。
3. Development and Evaluation of a Rapid Latex Agglutination Test Using a Monoclonal Antibody To Identify Candida dubliniensis Colonies [J] . Agnes Marot-Leblond, Bertrand Beucher, Sandrine David, Journal of Clinical Microbiology . 2006,第1期

机译：单克隆抗体鉴定dubliniensis菌落的快速乳胶凝集试验的开发和评估
4. COMPARISON OF A DEVELOPMENTAL ELISA FOR ANTI-Erysipelothrix rhusiopathiae ANTIBODIES WITH A PASSIVE AGGLUTINATION ASSAY USING LATEX BEADS COATED WITH A PURIFIED 64 kDa E. rhusiopathiae SPECIFIC PROTEI [C] . John C. Jones, Bobby L. Middlebrooks, Rhonda A. Patterson Annual Conference of the International Association for Aquatic Animal Medicine . 2001

机译：使用胶乳珠子涂覆纯化的64kDae.Rousiopathiae特异性Protei的胶乳珠抗肠杆菌抗体的发育ELISA对抗炎症抗体的抗体抗体的比较
5. Immunoglobulin variable region sequences of two human monoclonal antibodies directed to an onco-developmental carbohydrate antigen, lactotetraosylceramide(LcOse[4]Cer) [D] . Yago, Kazuhiro 1994

机译：两种针对人肿瘤发育碳水化合物抗原乳四糖神经酰胺（LcOse [4] Cer）的免疫球蛋白可变区序列
6. Development and Evaluation of a Rapid Latex Agglutination Test Using a Monoclonal Antibody To Identify Candida dubliniensis Colonies [O] . Agnes Marot-Leblond, Bertrand Beucher, Sandrine David, 2006

机译：单克隆抗体鉴定dubliniensis菌落的快速乳胶凝集试验的开发和评估
7. Development and Evaluation of a Rapid Latex Agglutination Test Using a Monoclonal Antibody To Identify Candida dubliniensis Colonies [O] . Marot-Leblond, Agnes, Beucher, Bertrand, David, Sandrine, 2006

机译：单克隆抗体鉴定dubliniensis菌落的快速乳胶凝集试验的开发和评估
8. Detection of Rotavirus with a New Polyclonal Antibody Enzyme Immunoassay (Rotazyme 2) and a Commercial Latex Agglutination Text (Rotalex): Comparison with a Monoclonal Antibody Enzyme Immunoassay [R] . Doern, G. V. , Herrmann, J. E. , Henderson, P. , 1986

机译：用新的多克隆抗体酶免疫分析（Rotazyme 2）和商业乳胶凝集文本（Rotalex）检测轮状病毒：与单克隆抗体酶免疫分析比较

Purification of immunoglobulins, their bonding onto latex particles and the development of latex agglutination test for mouse immunoglobulin-G for monoclonal antibody assessment.

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