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Analysis of DNA polymorphisms in the swine major histocompatibility complex (MHC) class I genes.

机译：猪主要组织相容性复合体（MHC）I类基因的DNA多态性分析。

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摘要

Performance data and blood samples from 70 Duroc and 38 Hampshire boars were collected from the 1986-87 national boar performance test of each breed. These boars represented a total of 97 litters from 73 farms. Few boars were inbred. The objectives of the study were: (1) to characterize the swine MHC class I genes by using restriction fragment length polymorphisms (RFLPs), (2) to determine the genetic variability within and between two breeds based on RFLPs of swine MHC class I genes and (3) to investigate the association between RFLP patterns of swine MHC class I genes and production traits in Duroc and Hampshire boars.;Genomic DNA was isolated from the white blood cells. Southern blotting and hybridization procedures were performed by using three different endonucleases and a swine MHC class I probe (PD1-A). The patterns of RFLPs of the two breeds showed a high degree of polymorphism and very few patterns were identical.;Jaccard coefficients, a similarity index, were used to measure the similarity between two RFLP patterns. Average Jaccard coefficients for each breed indicated that the similarity within breeds was higher than that between the two breeds and that the similarity within the Hampshire breed was higher than within the Duroc breed.;Nucleotide diversity, the average number of nucleotide differences per site between two DNA sequences, was calculated from RFLP patterns to measure the variability between two DNA sequences through RFLP patterns. The nucleotide diversity calculated within breed was lower than that between the two breeds and the diversity of Hampshire boars was lower than that of Duroc boars.;Least squares procedures and stepwise regression methods revealed that the associations exist between certain DNA restriction fragments and the selection index (INDEX), average daily gain (ADG), average backfat thickness (BF), loin muscle area (LEA) and age at 230 pounds (DAY230). These results suggest an association between swine MHC class I genes and performance traits in swine. Their use as genetic markers has potential for improving pig performance.
机译：从每个品种的1986-87年国家公猪性能测试中收集了70头杜洛克和38头汉普郡公猪的性能数据和血液样本。这些公猪代表了来自73个农场的97窝。自交的野猪很少。该研究的目的是：（1）通过使用限制性片段长度多态性（RFLP）表征猪MHC I类基因，（2）基于猪MHC I类基因的RFLP确定两个品种之间及其之间的遗传变异性。 （3）研究猪MHC I类基因的RFLP模式与杜洛克和汉普郡公猪的生产性状之间的关系。从白细胞中分离出基因组DNA。通过使用三种不同的核酸内切酶和猪MHC I类探针（PD1-A）进行Southern杂交和杂交程序。两个品种的RFLP图谱显示出高度的多态性，并且几乎没有相同的图谱。用Jaccard系数（相似度指数）衡量两个RFLP图谱之间的相似度。每个品种的平均Jaccard系数表明，两个品种之间的相似性高于两个品种，汉普郡品种之间的相似性高于Duroc品种。;核苷酸多样性，两个位点之间每个位点的平均核苷酸差异数根据RFLP模式计算DNA序列，以通过RFLP模式测量两个DNA序列之间的变异性。品种内计算的核苷酸多样性低于两个品种之间的核苷酸多样性，汉普郡公猪的多样性低于杜洛克公猪的多样性。最小二乘程序和逐步回归方法表明某些DNA限制片段与选择指数之间存在关联（INDEX），平均日增重（ADG），平均背脂厚度（BF），腰部肌肉面积（LEA）和230磅时的年龄（DAY230）。这些结果表明，猪MHC I类基因与猪的性状之间存在关联。将其用作遗传标记具有改善猪性能的潜力。

著录项

  • 作者

    Jung, Young Chul.;

  • 作者单位

    Iowa State University.;

  • 授予单位 Iowa State University.;
  • 学科 Genetics.
  • 学位 Ph.D.
  • 年度 1987
  • 页码 134 p.
  • 总页数 134
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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