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首页> 外文学位 >ANGIOTENSIN CONVERTING ENZYME IN THE BRAIN, TESTIS, EPIDIDYMIS, PITUITARY GLAND AND ADRENAL GLAND (CAPTOPRIL, EUKEPHALIN CONVERTASE, PEPTIDASE, CORPUS STRIATUM, GUANIDINOETHYL MERCAPTOSUCCINIC ACID).
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ANGIOTENSIN CONVERTING ENZYME IN THE BRAIN, TESTIS, EPIDIDYMIS, PITUITARY GLAND AND ADRENAL GLAND (CAPTOPRIL, EUKEPHALIN CONVERTASE, PEPTIDASE, CORPUS STRIATUM, GUANIDINOETHYL MERCAPTOSUCCINIC ACID).

机译：大脑，睾丸，癫痫，垂体和肾上腺（CAPTOPRIL，EUKEPHALIN转化酶，PEPTIDASE，CORPUS STRIATUM，GUANIDINOETHYL MERCAPTOSUCCINICID酸）中的血管紧张素转化酶。

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摘要

('3)H Captopril binds to angiotensin converting enzyme (ACE) in rat tissue homogenates. The pharmacology, regional distribution and copurification of ('3)H captopril binding with enzymatic activity demonstrate the selectivity of ('3)H captopril labeling of ACE. ('3)H Captopril binding to purified ACE reveals differences in cationic dependence and anionic regulation between substrate catalysis and inhibitor recognition. ('3)H Captopril association with ACE is entropically driven.; The selectivity of ('3)H captopril binding permits autoradiographic localization of the ACE in the brain, male reproductive system, pituitary gland and adrenal gland. In the brain, ACE is visualized in a striatonigral neuronal pathway which develops between 1 and 7 d after birth. There is no evidence for endogenous angiotensin II or angiotensin II receptors in such a pathway. This localization was confirmed by immuno-histochemical studies with a monoclonal anti-rat lung ACE antibody. ACE purified from the corpus stiatum has a M(,r) of 165,000 whereas lung ACE has a M(,r) of 175,000. The only difference between the native conformations of lung and striatal ACE we have found is their cleavage of amidated peptides. Substance P is an amidated neuropeptide found in a striatonigral pathway which is cleaved in a unique manner by striatal ACE.; In the male reproductive system, ('3)H captopril associated silver grains are found over spermatid heads and in the lumen of seminiferous tubules in stages I-VIII and XII-XIV. The initial segment of the epididymis contains very low levels of ('3)H captopril binding, while the head to of the epididymis has intense epithelial labeling. In a progression to the tail of the epididymis epithelial labeling declines and luminal grains increase. Three types of epididymal ACE can be distinguished by immunological and solubility properties. Developmental and hypophesectomy studies confirm that particulate epididymal ACE is not derived from testicular ACE.; In the pituitary gland, ACE is localized to the posterior lobe and patches of the anterior lobe. The adrenal medulla contains moderate ACE levels while low levels are found in the adrenal cortex. Adrenal medullary ACE is increased after hypophysectomy and after reserpine treatment.; The general use of ligand binding techniques for the study of enzymes is demonstrated by the specific labeling of another enzyme, enkephaline convertase, in crude tissue homogenates by the inhibitor ('3)H GEMSA.
机译：（'3）H卡托普利与大鼠组织匀浆中的血管紧张素转化酶（ACE）结合。具有酶活性的（3）H卡托普利结合的药理学，区域分布和共纯化证明了（3）H卡托普利标记ACE的选择性。 （'3）H卡托普利与纯化的ACE的结合揭示了底物催化和抑制剂识别之间阳离子依赖性和阴离子调节的差异。 （'3）H卡托普利与ACE的结合是由熵驱动的。 （'3）H卡托普利结合的选择性允许ACE在大脑，雄性生殖系统，垂体和肾上腺中放射自显影。在大脑中，ACE在出生后1至7 d发育的纹状体神经元通路中可见。没有证据表明在这种途径中存在内源性血管紧张素II或血管紧张素II受体。通过使用单克隆抗大鼠肺ACE抗体的免疫组织化学研究证实了这种定位。从sti体纯化的ACE的M（，r）为165,000，而肺ACE的M（，r）为175,000。我们发现，肺和纹状体ACE的天然构型之间的唯一区别是它们对酰胺化肽的裂解。 P物质是在纹状体神经通路中发现的一种酰胺化的神经肽，其被纹状体ACE以独特的方式裂解。在雄性生殖系统中，I-VIII和XII-XIV期精子头和生精小管腔内发现（'3）H卡托普利相关的银粒。附睾的初始区段包含非常低水平的（'3）H卡托普利结合，而附睾的头部具有强烈的上皮标记。在向附睾尾部的发展过程中，上皮标记减少，腔颗粒增加。三种类型的附睾ACE可以通过免疫学和溶解度特性来区分。发育和下丘脑切除术研究证实附睾微粒性ACE并非源于睾丸ACE。在垂体中，ACE位于后叶和前叶斑块。肾上腺髓质含有中等水平的ACE，而肾上腺皮质水平较低。垂体切除术后和利血平治疗后，肾上腺髓质ACE增加。配体结合技术用于研究酶的一般用途已通过抑制剂（'3）H GEMSA在粗组织匀浆中特异性标记另一种酶，即脑啡啉转化酶来证明。

著录项

  • 作者

    STRITTMATTER, STEPHEN MARK.;

  • 作者单位

    The Johns Hopkins University.;

  • 授予单位 The Johns Hopkins University.;
  • 学科 Biology Neuroscience.
  • 学位 Ph.D.
  • 年度 1986
  • 页码 271 p.
  • 总页数 271
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 神经科学;
  • 关键词

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