• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >ALLOZYME VARIABILITY IN BRASSICA OLERACEA AND APPLICATIONS IN PLANT BREEDING (ISOZYMES).
【24h】

ALLOZYME VARIABILITY IN BRASSICA OLERACEA AND APPLICATIONS IN PLANT BREEDING (ISOZYMES).

机译：甘蓝的同工酶变异性及其在植物育种中的应用（同工酶）。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Variation was detected at various regions of the zymogram of six enzymes (phosphoglucomutase, alcohol dehydrogenase, phosphoglucoisomerase, leucine aminopeptidase, glutamate-oxalacetate transaminase and acid phosphatase) in Brassica oleracea plant extracts (Chapter 1). Six isozyme genes were identified after electrophoretic analysis of selfed progeny and backcrosses of putatively heterozygous and homozygous individuals. Two more loci were implied from sporophyte-gametophyte zymogram comparisons. The electromorphs found at two additional zones were preliminarily concluded to be intergenic heterodimers. No tight linkage was found among 17 pairs of these loci.; Variability at ten isozyme genes was studied in nineteen cole open pollinated varieties representing seven horticultural groups (Chapter 2). Results indicated a high level of intrapopulation variation and interpopulation diversification. The mating system of OP varieties was estimated to be approximately random.; Two applications of electrophoresis in B. oleracea breeding were studied in detail:; (a) Hybrid purity testing. The analysis of 65 commercial F(,1) hybrids at six isozymes showed that most of them (89%) could be tested for purity using isozyme markers (Chapter 3). Lack of fixation in the inbred lines appeared to be a widespread phenomenon in B. oleracea parental lines.; Two models were developed for the determination of the sib proportion when one or both inbreds segregate at the test locus (Chapter 4). The variance of the sampling distribution and optimal sample allocation in the hybrid and inbred populations were studied. The scope of use of the unfixed models was discussed.; The proportion of contaminants of eight commercial F(,1) hybrids was determined after analysis of a sample of seeds of each hybrid and corresponding inbred lines at four isozyme loci (Chapter 5). Contamination estimates ranged from 1.5 to 40.1% and were generally higher than grow-out estimates of the same hybrids. Isozymes were proposed as a tool for selecting homozygous and highly self-incompatible inbred lines.; (b) F(,1) hybrid identification. The isozyme phenotype can be used as a sensitive and rapid test of identity for F(,1) hybrids. The analysis of a sample of seeds of 65 F(,1) hybrids for six polymorphic loci was enough to individually distinguish 48 (73%) of them (Chapter 3).
机译：在芸苔属植物提取物中的六种酶（磷酸葡萄糖变位酶，乙醇脱氢酶，磷酸葡萄糖异构酶，亮氨酸氨基肽酶，谷氨酸-草酰乙酸转氨酶和酸性磷酸酶）的酶谱图的不同区域检测到了变化（第1章）。电泳分析自体子代和假定杂合和纯合个体的回交后，鉴定出六个同工酶基因。孢子-配子体酶谱比较暗示了另外两个基因座。初步推断在两个其他区域发现的电同质子是基因间异二聚体。在这些基因座的17对之间未发现紧密的连锁关系。在代表七个园艺组的19个油菜开放授粉品种中研究了10个同工酶基因的变异性（第2章）。结果表明高水平的种群内变异和种群间多样化。 OP品种的交配系统估计是近似随机的。详细研究了电泳在B. oleracea育种中的两种应用： （a）混合纯度测试。对65种商业F（，1）杂种的6种同工酶的分析表明，可以使用同工酶标记物对大多数杂种（89％）进行纯度测试（第3章）。自交系缺乏固定似乎是油菜双亲亲本系中普遍存在的现象。当一个或两个自交系在测试位点分离时，开发了两个模型来确定同胞比例（第4章）。研究了杂种和近交种群的抽样分布和最佳样本分配的方差。讨论了未固定模型的使用范围。在分析每个杂种的种子样本和相应的近交系的四个同工酶基因座后，确定了八个商业F（，1）杂种的污染物比例（第5章）。污染估计范围为1.5％至40.1％，通常高于相同杂种的成年估计。提出了同工酶作为选择纯合和高度自我不相容的近交系的工具。 （b）F（，1）混合标识。同工酶表型可以用作F（，1）杂种的灵敏和快速的身份测试。对65个F（，1）杂种种子的六个多态位点的样品进行分析足以区分出48个（73％）（第3章）。

著录项

  • 作者

    ARUS, PERE.;

  • 作者单位

    University of California, Davis.;

  • 授予单位 University of California, Davis.;
  • 学科 Biology Genetics.; Agriculture Plant Culture.
  • 学位 Ph.D.
  • 年度 1984
  • 页码 169 p.
  • 总页数 169
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;作物生物学原理、栽培技术与方法;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号