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首页> 外文学位 >STUDIES ON THE DEVELOPMENT OF INSTRUMENTAL INSEMINATION OF QUEEN HONEY BEES (APIS MELLIFERA L.): SEMEN COLLECTION, STORAGE AND THEIR EFFECTS ON THE ONSET OF OVIPOSITION.
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STUDIES ON THE DEVELOPMENT OF INSTRUMENTAL INSEMINATION OF QUEEN HONEY BEES (APIS MELLIFERA L.): SEMEN COLLECTION, STORAGE AND THEIR EFFECTS ON THE ONSET OF OVIPOSITION.

机译：蜂王用蜜蜂人工授精的研究：精液的收集，贮藏及其对产卵的影响。

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摘要

The new syringe described was designed for use in both semen storage and instrumental insemination. The Mackensen syringe tip was modified to accommodate a capillary storage in its base, and a syringe barrel with a screw-type plunger was deviced for precise control of the movement of semen. The capillary tubes containing semen can be removed from the syringe and replaced without disturbing the column of semen, by equalizing the pressure in the barrel and in the storage tubes. The capillary tubes can be loaded with enough semen to inseminate several queens.; A simple and fast method for collecting honeybee semen in large quantity is described. Semen and mucus of many drones, everted and ejaculated by hand, were scraped into Kiev diluent in a semen-washing funnel and a collecting tube. The semen-mucus-diluent mixture was centrifuged at 2500 rpm for 10 minute to separate semen from mucus and the diluent. The collecting tube containing was incorporated in to a special large-capacity syringe, and the semen was used to inseminate queen honeybees.; No significant differences were found in the amount of brood produced between 31 queens instrumentally inseminated with semen collected by this method, queens inseminated with semen collected by the conventional Mackensen technique, and open-mated queens (P > 0.05 in all cases). However, significant differences were observed in the onset of oviposition (P < 0.05 in all cases). There was a delay before oviposition by all the instrumentally inseminated queens, which was greatest for queens inseminated with semen collected by the washing technique (P < 0.05). Effects of washing and centrifugation of semen are discussed.; The effects of washing honey bee (Apis mellifera L.) semen and carbon dioxide treatments on the onset of oviposition of naturally mated and/or instrumentally inseminated queen honey bees were determined. The number of days from emergence to the onset of oviposition of 7 groups of queens was as follows: naturally mated queens, 10.33 (+OR-) 0.68; free flying queens treated with CO(,2), 11.00 (+OR-) 0.36; queens instrumentally inseminated with Mackensen technique, 13.8 (+OR-) 1.94; virgin queens treated with CO(,2), 14.00 (+OR-) 0.77; queens instrumentally inseminated with washing technique, 14.58 (+OR-) 0.53; queens injected with washing fluid, 15.82 (+OR-) 1.42; and queens injected with Kiev solution, 17.77 (+OR-) 1.24. . . . (Author's abstract exceeds stipulated maximum length. Discontinued here with permission of author.) UMI
机译：所描述的新注射器设计用于精液储存和人工授精。修改了Mackensen注射器针尖，使其底部容纳了毛细管，并装有带有螺杆式柱塞的注射器针筒，用于精确控制精液的运动。通过平衡针管和储存管中的压力，可以从注射器中取出包含精液的毛细管，并进行更换，而不会影响精液柱。毛细管可以装满足够的精液，以授精几个皇后。描述了一种用于收集大量蜜蜂精液的简单快速的方法。许多精子和粘液经人工外翻和射精后，在精液洗涤漏斗和收集管中刮入基辅稀释剂中。将精液-粘液-稀释剂混合物以2500 rpm离心10分钟，以将精液与粘液和稀释剂分离。将装有收集管的收集管装入一个特殊的大容量注射器中，并将精液用于授精女王蜂。在用这种方法收集的精液用工具人工授精的31只皇后，用常规的Mackensen技术收集的精液授精的皇后和开放型的皇后之间，产卵量没有显着差异（在所有情况下P> 0.05）。然而，在产卵开始时观察到显着差异（在所有情况下，P <0.05）。所有用工具进行授精的皇后在产卵前都有一个延迟，这对用洗涤技术收集的精液授精的皇后最为明显（P <0.05）。讨论了精液的洗涤和离心作用。确定了洗蜜蜂（Apis mellifera L.）精液和二氧化碳处理对自然交配和/或人工授精的女王蜂产卵的影响。从出现到出现女王的7组女王的天数如下：自然交配的女王，10.33（+ OR-）0.68；用CO（，2），11.00（+ OR-）0.36处理的自由飞行女王;女王用麦肯森技术进行了人工授精，为13.8（+ OR-）1.94；用CO（，2），14.00（+ OR-）0.77处理的原始皇后;皇后使用洗涤技术人工授精，14.58（+ OR-）0.53；皇后区注入清洗液15.82（+ OR-）1.42;和皇后区分别注入基辅溶液17.77（+ OR-）1.24。 。 。 。 （作者的摘要超出了规定的最大长度。经作者许可，在此处停产。）UMI

著录项

  • 作者

    KAFTANOGLU, OSMAN.;

  • 作者单位

    University of California, Davis.;

  • 授予单位 University of California, Davis.;
  • 学科 Biology Entomology.
  • 学位 Ph.D.
  • 年度 1983
  • 页码 83 p.
  • 总页数 83
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 昆虫学;
  • 关键词

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